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In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens.
Pak J Pharm Sci. 2012 Apr; 25(2):357-64.PJ

Abstract

Methanolic extract of Boerhavia procumbens Bank ex Roxb. was partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially after dissolving in distilled water. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol soluble fraction. The antioxidant activity of all these fractions and the remaining aqueous fraction was evaluated by four methods such as: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity and ferric thiocyanate assay. Total phenolics were also determined. Some fractions showed noteworthy antioxidant activity. The results of the antioxidant activity revealed that the ethyl acetate soluble fraction showed the highest value of percent inhibition of DPPH (82.54 ± 0.62) at the concentration of 125 μ g/ml. The IC(50) of this fraction was 37.11± 0.23 μg/ml, compared with butylated hydroxytoluene (BHT), which have IC(50) of 12.1 ± 0.92 μ/mL. It also showed the highest FRAP value (251.08 ± 1.46 μg of trolox equivalents) as well as the highest value of lipid peroxidation inhibition (57.21 ± 52%), the highest total antioxidant activity (0.549 ± 0.08) and also the highest total phenolic contents (77.1 ± 0.6) as compared to the studied fractions. Phytochemical screening showed high percentage of phenolics, flavonoides and cardiac glycosides in this fraction.

Authors+Show Affiliations

Department of Chemistry, Government College University, Lahore, Pakistan. atrabbasi@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22459462

Citation

Abbasi, Muhammad Athar, et al. "In Vitro Assessment of Relief to Oxidative Stress By Different Fractions of Boerhavia Procumbens." Pakistan Journal of Pharmaceutical Sciences, vol. 25, no. 2, 2012, pp. 357-64.
Abbasi MA, Rubab K, Rehman A, et al. In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens. Pak J Pharm Sci. 2012;25(2):357-64.
Abbasi, M. A., Rubab, K., Rehman, A., Riaz, T., Shahzadi, T., Khalid, M., & Ajaib, M. (2012). In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens. Pakistan Journal of Pharmaceutical Sciences, 25(2), 357-64.
Abbasi MA, et al. In Vitro Assessment of Relief to Oxidative Stress By Different Fractions of Boerhavia Procumbens. Pak J Pharm Sci. 2012;25(2):357-64. PubMed PMID: 22459462.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro assessment of relief to oxidative stress by different fractions of Boerhavia procumbens. AU - Abbasi,Muhammad Athar, AU - Rubab,Kaniz, AU - Rehman,Azizur, AU - Riaz,Tauheeda, AU - Shahzadi,Tayyaba, AU - Khalid,Muniba, AU - Ajaib,Muhammad, PY - 2012/3/31/entrez PY - 2012/3/31/pubmed PY - 2012/6/12/medline SP - 357 EP - 64 JF - Pakistan journal of pharmaceutical sciences JO - Pak J Pharm Sci VL - 25 IS - 2 N2 - Methanolic extract of Boerhavia procumbens Bank ex Roxb. was partitioned with n-hexane, chloroform, ethyl acetate and n-butanol sequentially after dissolving in distilled water. Phytochemical screening showed presence of phenolics, flavonoides and cardiac glycosides in large amount in chloroform, ethyl acetate and n-butanol soluble fraction. The antioxidant activity of all these fractions and the remaining aqueous fraction was evaluated by four methods such as: 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging activity, ferric reducing antioxidant power (FRAP) assay, total antioxidant activity and ferric thiocyanate assay. Total phenolics were also determined. Some fractions showed noteworthy antioxidant activity. The results of the antioxidant activity revealed that the ethyl acetate soluble fraction showed the highest value of percent inhibition of DPPH (82.54 ± 0.62) at the concentration of 125 μ g/ml. The IC(50) of this fraction was 37.11± 0.23 μg/ml, compared with butylated hydroxytoluene (BHT), which have IC(50) of 12.1 ± 0.92 μ/mL. It also showed the highest FRAP value (251.08 ± 1.46 μg of trolox equivalents) as well as the highest value of lipid peroxidation inhibition (57.21 ± 52%), the highest total antioxidant activity (0.549 ± 0.08) and also the highest total phenolic contents (77.1 ± 0.6) as compared to the studied fractions. Phytochemical screening showed high percentage of phenolics, flavonoides and cardiac glycosides in this fraction. SN - 1011-601X UR - https://www.unboundmedicine.com/medline/citation/22459462/In_vitro_assessment_of_relief_to_oxidative_stress_by_different_fractions_of_Boerhavia_procumbens_ L2 - https://antibodies.cancer.gov/detail/CPTC-MTOR-8 DB - PRIME DP - Unbound Medicine ER -