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Development of a simultaneous analytical method for selected anorectics, methamphetamine, MDMA, and their metabolites in hair using LC-MS/MS to prove anorectics abuse.
Anal Bioanal Chem. 2012 May; 403(5):1385-94.AB

Abstract

Owing to the tight control of methamphetamine, it is presumed that phentermine, an amphetamine-type anorectic, has recently been considered a supplement for methamphetamine abusers in Korea. In addition, the abuse of other anorectics obtained by inappropriate means has become a social issue. Hair is a useful specimen to prove chronic drug use. Therefore, an analytical method for the simultaneous detection of phentermine, phendimetrazine, amfepramone, fenfluramine, mazindol, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA), as well as their metabolites, which covers the major amphetamines and anorectic agents in Korea, in hair was established and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The drugs and their metabolites in hair were extracted using 1 % HCl in methanol and then filtered and analyzed by LC-MS/MS with electrospray ionization in positive mode. The validation results for selectivity, linearity, matrix effect, recovery, process efficiency, intra- and interassay precision and accuracy, and processed sample stability were satisfactory. The limits of detection ranged from 0.025 to 1 ng/10 mg hair and the limits of quantification were 0.25 ng/10 mg hair for every analyte except mazindol and phentermine, for which they were 10 ng/10 mg hair. The method was successfully applied for the segmental determination of selected anorectics, methamphetamine, MDMA, and their metabolites in hair from 39 drug suspects. Among the anorectics, phentermine and/or phendimetrazine were identified with or without methamphetamine in the hair samples. Closer supervision of the inappropriate use of anorectics is necessary. Also, hair analysis is useful for monitoring the abuse potential of unnoticed drugs.

Authors+Show Affiliations

College of Pharmacy, Keimyung University, Dalseo-gu, Daegu, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22460079

Citation

Lee, Sooyeun, et al. "Development of a Simultaneous Analytical Method for Selected Anorectics, Methamphetamine, MDMA, and Their Metabolites in Hair Using LC-MS/MS to Prove Anorectics Abuse." Analytical and Bioanalytical Chemistry, vol. 403, no. 5, 2012, pp. 1385-94.
Lee S, Kim J, In S, et al. Development of a simultaneous analytical method for selected anorectics, methamphetamine, MDMA, and their metabolites in hair using LC-MS/MS to prove anorectics abuse. Anal Bioanal Chem. 2012;403(5):1385-94.
Lee, S., Kim, J., In, S., Choi, H., Oh, S. M., Jang, C. G., & Chung, K. H. (2012). Development of a simultaneous analytical method for selected anorectics, methamphetamine, MDMA, and their metabolites in hair using LC-MS/MS to prove anorectics abuse. Analytical and Bioanalytical Chemistry, 403(5), 1385-94. https://doi.org/10.1007/s00216-012-5950-6
Lee S, et al. Development of a Simultaneous Analytical Method for Selected Anorectics, Methamphetamine, MDMA, and Their Metabolites in Hair Using LC-MS/MS to Prove Anorectics Abuse. Anal Bioanal Chem. 2012;403(5):1385-94. PubMed PMID: 22460079.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of a simultaneous analytical method for selected anorectics, methamphetamine, MDMA, and their metabolites in hair using LC-MS/MS to prove anorectics abuse. AU - Lee,Sooyeun, AU - Kim,Jihyun, AU - In,Sanghwan, AU - Choi,Hwakyung, AU - Oh,Seung Min, AU - Jang,Choon-Gon, AU - Chung,Kyu Hyuck, Y1 - 2012/03/30/ PY - 2011/11/25/received PY - 2012/03/13/accepted PY - 2012/01/31/revised PY - 2012/3/31/entrez PY - 2012/3/31/pubmed PY - 2012/8/14/medline SP - 1385 EP - 94 JF - Analytical and bioanalytical chemistry JO - Anal Bioanal Chem VL - 403 IS - 5 N2 - Owing to the tight control of methamphetamine, it is presumed that phentermine, an amphetamine-type anorectic, has recently been considered a supplement for methamphetamine abusers in Korea. In addition, the abuse of other anorectics obtained by inappropriate means has become a social issue. Hair is a useful specimen to prove chronic drug use. Therefore, an analytical method for the simultaneous detection of phentermine, phendimetrazine, amfepramone, fenfluramine, mazindol, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA), as well as their metabolites, which covers the major amphetamines and anorectic agents in Korea, in hair was established and validated using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The drugs and their metabolites in hair were extracted using 1 % HCl in methanol and then filtered and analyzed by LC-MS/MS with electrospray ionization in positive mode. The validation results for selectivity, linearity, matrix effect, recovery, process efficiency, intra- and interassay precision and accuracy, and processed sample stability were satisfactory. The limits of detection ranged from 0.025 to 1 ng/10 mg hair and the limits of quantification were 0.25 ng/10 mg hair for every analyte except mazindol and phentermine, for which they were 10 ng/10 mg hair. The method was successfully applied for the segmental determination of selected anorectics, methamphetamine, MDMA, and their metabolites in hair from 39 drug suspects. Among the anorectics, phentermine and/or phendimetrazine were identified with or without methamphetamine in the hair samples. Closer supervision of the inappropriate use of anorectics is necessary. Also, hair analysis is useful for monitoring the abuse potential of unnoticed drugs. SN - 1618-2650 UR - https://www.unboundmedicine.com/medline/citation/22460079/Development_of_a_simultaneous_analytical_method_for_selected_anorectics_methamphetamine_MDMA_and_their_metabolites_in_hair_using_LC_MS/MS_to_prove_anorectics_abuse_ L2 - https://dx.doi.org/10.1007/s00216-012-5950-6 DB - PRIME DP - Unbound Medicine ER -