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PDGF-α stimulates intestinal epithelial cell turnover after massive small bowel resection in a rat.
Am J Physiol Gastrointest Liver Physiol. 2012 Jun 01; 302(11):G1274-81.AJ

Abstract

Numerous cytokines have been shown to affect epithelial cell differentiation and proliferation through epithelial-mesenchymal interaction. Growing evidence suggests that platelet-derived growth factor (PDGF) signaling is an important mediator of these interactions. The purpose of this study was to evaluate the effect of PDGF-α on enterocyte turnover in a rat model of short bowel syndrome (SBS). Male rats were divided into four groups: Sham rats underwent bowel transection, Sham-PDGF-α rats underwent bowel transection and were treated with PDGF-α, SBS rats underwent a 75% bowel resection, and SBS-PDGF-α rats underwent bowel resection and were treated with PDGF-α. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined at euthanasia. Illumina's Digital Gene Expression analysis was used to determine PDGF-related gene expression profiling. PDGF-α and PDGF-α receptor (PDGFR-α) expression was determined by real-time PCR. Western blotting was used to determine p-ERK, Akt1/2/3, bax, and bcl-2 protein levels. SBS rats demonstrated a significant increase in PDGF-α and PDGFR-α expression in jejunum and ileum compared with sham animals. SBS-PDGF-α rats demonstrated a significant increase in bowel and mucosal weight, villus height, and crypt depth in jejunum and ileum compared with SBS animals. PDGF-α receptor expression in crypts increased in SBS rats (vs. sham) and was accompanied by an increased cell proliferation following PDGF-α administration. A significant decrease in cell apoptosis in this group was correlated with lower bax protein levels. In conclusion, in a rat model of SBS, PDGF-α stimulates enterocyte turnover, which is correlated with upregulated PDGF-α receptor expression in the remaining small intestine.

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Authors+Show Affiliations

Sukhotnik I
Technion-Israel Institute of Technology, the Ruth and Bruce Rappaport Faculty of Medicine, Laboratory of Intestinal Adaptation and Recovery, Bnai Zion Medical Center, Haifa, Israel. igor-dr@internet-zahav.net
Mogilner JG
No affiliation info available
Pollak Y
No affiliation info available
Blumenfeld S
No affiliation info available
Bejar J
No affiliation info available
Coran AG
No affiliation info available

MeSH

AnimalsApoptosisCell ProliferationDisease Models, AnimalEnterocytesGene ExpressionIntestinal MucosaIntestine, SmallMalePlatelet-Derived Growth FactorRatsRats, Sprague-DawleyReceptor, Platelet-Derived Growth Factor alphaShort Bowel Syndromebcl-2-Associated X Protein

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22461028

Citation

Sukhotnik, Igor, et al. "PDGF-α Stimulates Intestinal Epithelial Cell Turnover After Massive Small Bowel Resection in a Rat." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 302, no. 11, 2012, pp. G1274-81.
Sukhotnik I, Mogilner JG, Pollak Y, et al. PDGF-α stimulates intestinal epithelial cell turnover after massive small bowel resection in a rat. Am J Physiol Gastrointest Liver Physiol. 2012;302(11):G1274-81.
Sukhotnik, I., Mogilner, J. G., Pollak, Y., Blumenfeld, S., Bejar, J., & Coran, A. G. (2012). PDGF-α stimulates intestinal epithelial cell turnover after massive small bowel resection in a rat. American Journal of Physiology. Gastrointestinal and Liver Physiology, 302(11), G1274-81. https://doi.org/10.1152/ajpgi.00532.2011
Sukhotnik I, et al. PDGF-α Stimulates Intestinal Epithelial Cell Turnover After Massive Small Bowel Resection in a Rat. Am J Physiol Gastrointest Liver Physiol. 2012 Jun 1;302(11):G1274-81. PubMed PMID: 22461028.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - PDGF-α stimulates intestinal epithelial cell turnover after massive small bowel resection in a rat. AU - Sukhotnik,Igor, AU - Mogilner,Jorge G, AU - Pollak,Yulia, AU - Blumenfeld,Shiri, AU - Bejar,Jacob, AU - Coran,Arnold G, Y1 - 2012/03/29/ PY - 2012/3/31/entrez PY - 2012/3/31/pubmed PY - 2012/8/11/medline SP - G1274 EP - 81 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am J Physiol Gastrointest Liver Physiol VL - 302 IS - 11 N2 - Numerous cytokines have been shown to affect epithelial cell differentiation and proliferation through epithelial-mesenchymal interaction. Growing evidence suggests that platelet-derived growth factor (PDGF) signaling is an important mediator of these interactions. The purpose of this study was to evaluate the effect of PDGF-α on enterocyte turnover in a rat model of short bowel syndrome (SBS). Male rats were divided into four groups: Sham rats underwent bowel transection, Sham-PDGF-α rats underwent bowel transection and were treated with PDGF-α, SBS rats underwent a 75% bowel resection, and SBS-PDGF-α rats underwent bowel resection and were treated with PDGF-α. Parameters of intestinal adaptation, enterocyte proliferation and apoptosis were determined at euthanasia. Illumina's Digital Gene Expression analysis was used to determine PDGF-related gene expression profiling. PDGF-α and PDGF-α receptor (PDGFR-α) expression was determined by real-time PCR. Western blotting was used to determine p-ERK, Akt1/2/3, bax, and bcl-2 protein levels. SBS rats demonstrated a significant increase in PDGF-α and PDGFR-α expression in jejunum and ileum compared with sham animals. SBS-PDGF-α rats demonstrated a significant increase in bowel and mucosal weight, villus height, and crypt depth in jejunum and ileum compared with SBS animals. PDGF-α receptor expression in crypts increased in SBS rats (vs. sham) and was accompanied by an increased cell proliferation following PDGF-α administration. A significant decrease in cell apoptosis in this group was correlated with lower bax protein levels. In conclusion, in a rat model of SBS, PDGF-α stimulates enterocyte turnover, which is correlated with upregulated PDGF-α receptor expression in the remaining small intestine. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/22461028/PDGF_α_stimulates_intestinal_epithelial_cell_turnover_after_massive_small_bowel_resection_in_a_rat_ L2 - https://journals.physiology.org/doi/10.1152/ajpgi.00532.2011?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -