Tags

Type your tag names separated by a space and hit enter

Onion peel extract increases hepatic low-density lipoprotein receptor and ATP-binding cassette transporter A1 messenger RNA expressions in Sprague-Dawley rats fed a high-fat diet.
Nutr Res. 2012 Mar; 32(3):210-7.NR

Abstract

In the present study, we hypothesized that onion peel extract (OPE) alters hepatic gene expression to improve blood cholesterol profiles. To investigate the effect of OPE to test our hypothesis, Sprague-Dawley rats were fed ad libitum for 8 weeks with the control, high-fat diet (HFD) or the high-fat diet with 0.2% OPE supplementations (HFD + OPE). Messenger RNA (mRNA) levels of genes in cholesterol metabolism and fatty acid metabolism were examined by semiquantitative reverse transcriptase polymerase chain reaction. The OPE in HFD reverted high fat-induced reduction in mRNA levels of sterol regulatory element-binding protein-2, low-density lipoprotein receptor, and hydroxyl-3-methylglutaryl coenzyme reductase genes in the liver comparable with the levels of the control group. Onion peel extract slightly increased stearoyl-coA desaturase 1 (SCD-1) expression compared with high-fat feeding. However, sterol regulatory element-binding protein-1c and fatty acid synthase were not affected by high-fat or OPE feeding. Onion peel extract also enhanced expression of ATP-binding cassette transporter A1, peroxisome proliferator-activated receptor γ2 and scavenger receptor class B type I genes when compared with high-fat feeding. However, OPE did not influence high fat-triggered changes in apolipoprotein A1 mRNA levels and liver X receptor α were not affected by either high-fat or OPE feeding. Our results suggest that OPE changes the expression of genes associated with cholesterol metabolism in favor of lowering blood low-density lipoprotein cholesterol and enhancing high-density lipoprotein cholesterol through increasing mRNA abundance of low-density lipoprotein receptor and ATP-binding cassette transporter A1 genes.

Authors+Show Affiliations

Department of Food and Nutrition, Institute of Health Sciences, Korea University, Seoul, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22464808

Citation

Lee, Seung-Min, et al. "Onion Peel Extract Increases Hepatic Low-density Lipoprotein Receptor and ATP-binding Cassette Transporter A1 Messenger RNA Expressions in Sprague-Dawley Rats Fed a High-fat Diet." Nutrition Research (New York, N.Y.), vol. 32, no. 3, 2012, pp. 210-7.
Lee SM, Moon J, Do HJ, et al. Onion peel extract increases hepatic low-density lipoprotein receptor and ATP-binding cassette transporter A1 messenger RNA expressions in Sprague-Dawley rats fed a high-fat diet. Nutr Res. 2012;32(3):210-7.
Lee, S. M., Moon, J., Do, H. J., Chung, J. H., Lee, K. H., Cha, Y. J., & Shin, M. J. (2012). Onion peel extract increases hepatic low-density lipoprotein receptor and ATP-binding cassette transporter A1 messenger RNA expressions in Sprague-Dawley rats fed a high-fat diet. Nutrition Research (New York, N.Y.), 32(3), 210-7. https://doi.org/10.1016/j.nutres.2012.01.004
Lee SM, et al. Onion Peel Extract Increases Hepatic Low-density Lipoprotein Receptor and ATP-binding Cassette Transporter A1 Messenger RNA Expressions in Sprague-Dawley Rats Fed a High-fat Diet. Nutr Res. 2012;32(3):210-7. PubMed PMID: 22464808.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Onion peel extract increases hepatic low-density lipoprotein receptor and ATP-binding cassette transporter A1 messenger RNA expressions in Sprague-Dawley rats fed a high-fat diet. AU - Lee,Seung-Min, AU - Moon,Jiyoung, AU - Do,Hyun Ju, AU - Chung,Ji Hyung, AU - Lee,Kyung-Hea, AU - Cha,Yong-Jun, AU - Shin,Min-Jeong, PY - 2011/08/25/received PY - 2012/01/09/revised PY - 2012/01/18/accepted PY - 2012/4/3/entrez PY - 2012/4/3/pubmed PY - 2012/7/28/medline SP - 210 EP - 7 JF - Nutrition research (New York, N.Y.) JO - Nutr Res VL - 32 IS - 3 N2 - In the present study, we hypothesized that onion peel extract (OPE) alters hepatic gene expression to improve blood cholesterol profiles. To investigate the effect of OPE to test our hypothesis, Sprague-Dawley rats were fed ad libitum for 8 weeks with the control, high-fat diet (HFD) or the high-fat diet with 0.2% OPE supplementations (HFD + OPE). Messenger RNA (mRNA) levels of genes in cholesterol metabolism and fatty acid metabolism were examined by semiquantitative reverse transcriptase polymerase chain reaction. The OPE in HFD reverted high fat-induced reduction in mRNA levels of sterol regulatory element-binding protein-2, low-density lipoprotein receptor, and hydroxyl-3-methylglutaryl coenzyme reductase genes in the liver comparable with the levels of the control group. Onion peel extract slightly increased stearoyl-coA desaturase 1 (SCD-1) expression compared with high-fat feeding. However, sterol regulatory element-binding protein-1c and fatty acid synthase were not affected by high-fat or OPE feeding. Onion peel extract also enhanced expression of ATP-binding cassette transporter A1, peroxisome proliferator-activated receptor γ2 and scavenger receptor class B type I genes when compared with high-fat feeding. However, OPE did not influence high fat-triggered changes in apolipoprotein A1 mRNA levels and liver X receptor α were not affected by either high-fat or OPE feeding. Our results suggest that OPE changes the expression of genes associated with cholesterol metabolism in favor of lowering blood low-density lipoprotein cholesterol and enhancing high-density lipoprotein cholesterol through increasing mRNA abundance of low-density lipoprotein receptor and ATP-binding cassette transporter A1 genes. SN - 1879-0739 UR - https://www.unboundmedicine.com/medline/citation/22464808/Onion_peel_extract_increases_hepatic_low_density_lipoprotein_receptor_and_ATP_binding_cassette_transporter_A1_messenger_RNA_expressions_in_Sprague_Dawley_rats_fed_a_high_fat_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0271-5317(12)00026-7 DB - PRIME DP - Unbound Medicine ER -