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Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells.
J Ethnopharmacol 2012; 141(1):510-6JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Bryonia dioica Jacq. is a climbing perennial herb with tuberous roots which is widely used in traditional medicine in Algeria for the treatment of cancers; it belongs to the genus Bryonia (Cucurbitaceae).

AIM OF THE STUDY

To investigate the cytotoxic and apoptogenic activities, the phytochemical composition and acute toxicity of the aqueous extract of Bryonia dioica roots growing in Algeria.

MATERIALS AND METHODS

Dried roots of Bryonia dioica were extracted with water (decoction). The cytotoxic effects of the aqueous extract in the Burkitt's lymphoma BL41 cell lines were evaluated by flow cytometry. Apoptosis induction was assessed by two corroborative assays; propidium iodide (PI) staining of cell DNA and flow cytometric light scatter analysis. The mitochondria membrane potential was investigated using a fluorescent dye DIOC6. The expression of caspases-3, -8, -9 and PARP was assessed by Western blot. The phytochemical screening of the roots of Bryonia dioica was performed using qualitative phytochemical standard procedures.

RESULTS

The Bryonia dioica aqueous extract induced cell death in a dose-dependent manner. The IC50 of Bryonia dioica aqueous extract was estimated to be approximately 15, 63μg/ml. This was accompanied by induction of apoptosis, activation of caspase-3 and -9, cleavage of PARP and loss of mitochondria membrane potential. Furthermore, the phytochemical screening of roots of Bryonia dioica showed the presence of various bioactive such as polyphenols, sterols and triterpenes, alkaloids, c-heterosides, carbohydrates and saponins.

CONCLUSION

The aqueous extract of Bryonia dioica induces apoptosis in the Burkitt's lymphoma BL41 cell lines via the mitochondrial pathway. The flavonoids, sterols and triterpens detected could be responsible for the cytotoxic and apoptogenic activities of the aqueous extract of Bryonia dioica. These findings suggest that Bryonia dioica could be considered as a promising source for developing novel therapeutics against Burkitt's lymphoma.

Authors+Show Affiliations

Laboratory Research on Biological Systems and Geomatics, Faculty of Nature and Life, University of Mascara, Algeria. bachirsb@yahoo.frNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22465729

Citation

Benarba, Bachir, et al. "Bryonia Dioica Aqueous Extract Induces Apoptosis Through Mitochondrial Intrinsic Pathway in BL41 Burkitt's Lymphoma Cells." Journal of Ethnopharmacology, vol. 141, no. 1, 2012, pp. 510-6.
Benarba B, Meddah B, Aoues A. Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells. J Ethnopharmacol. 2012;141(1):510-6.
Benarba, B., Meddah, B., & Aoues, A. (2012). Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells. Journal of Ethnopharmacology, 141(1), pp. 510-6. doi:10.1016/j.jep.2012.02.052.
Benarba B, Meddah B, Aoues A. Bryonia Dioica Aqueous Extract Induces Apoptosis Through Mitochondrial Intrinsic Pathway in BL41 Burkitt's Lymphoma Cells. J Ethnopharmacol. 2012 May 7;141(1):510-6. PubMed PMID: 22465729.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells. AU - Benarba,Bachir, AU - Meddah,Boumedienne, AU - Aoues,Abdelkader, Y1 - 2012/03/21/ PY - 2011/08/20/received PY - 2012/01/16/revised PY - 2012/02/07/accepted PY - 2012/4/3/entrez PY - 2012/4/3/pubmed PY - 2012/8/11/medline SP - 510 EP - 6 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Bryonia dioica Jacq. is a climbing perennial herb with tuberous roots which is widely used in traditional medicine in Algeria for the treatment of cancers; it belongs to the genus Bryonia (Cucurbitaceae). AIM OF THE STUDY: To investigate the cytotoxic and apoptogenic activities, the phytochemical composition and acute toxicity of the aqueous extract of Bryonia dioica roots growing in Algeria. MATERIALS AND METHODS: Dried roots of Bryonia dioica were extracted with water (decoction). The cytotoxic effects of the aqueous extract in the Burkitt's lymphoma BL41 cell lines were evaluated by flow cytometry. Apoptosis induction was assessed by two corroborative assays; propidium iodide (PI) staining of cell DNA and flow cytometric light scatter analysis. The mitochondria membrane potential was investigated using a fluorescent dye DIOC6. The expression of caspases-3, -8, -9 and PARP was assessed by Western blot. The phytochemical screening of the roots of Bryonia dioica was performed using qualitative phytochemical standard procedures. RESULTS: The Bryonia dioica aqueous extract induced cell death in a dose-dependent manner. The IC50 of Bryonia dioica aqueous extract was estimated to be approximately 15, 63μg/ml. This was accompanied by induction of apoptosis, activation of caspase-3 and -9, cleavage of PARP and loss of mitochondria membrane potential. Furthermore, the phytochemical screening of roots of Bryonia dioica showed the presence of various bioactive such as polyphenols, sterols and triterpenes, alkaloids, c-heterosides, carbohydrates and saponins. CONCLUSION: The aqueous extract of Bryonia dioica induces apoptosis in the Burkitt's lymphoma BL41 cell lines via the mitochondrial pathway. The flavonoids, sterols and triterpens detected could be responsible for the cytotoxic and apoptogenic activities of the aqueous extract of Bryonia dioica. These findings suggest that Bryonia dioica could be considered as a promising source for developing novel therapeutics against Burkitt's lymphoma. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22465729/Bryonia_dioica_aqueous_extract_induces_apoptosis_through_mitochondrial_intrinsic_pathway_in_BL41_Burkitt's_lymphoma_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00179-1 DB - PRIME DP - Unbound Medicine ER -