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Cochinchina momordica seed extract induces G2/M arrest and apoptosis in human breast cancer MDA-MB-231 cells by modulating the PI3K/Akt pathway.
Asian Pac J Cancer Prev. 2011; 12(12):3483-8.AP

Abstract

Cochinchina momordica seeds are a kind of traditional Chinese herb. In this study, anticancer activity and underlying mechanisms were investigated with an extract using human breast cancer MDA-MB-231 cells. The survival rate was reduced in a concentration- and time-dependent manner as assessed by MTT assay. After incubation for 48 h, typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. Flow cytometry revealed that the treatment obviously induced G2/M arrest and apoptosis in MDA-MB-231 cells. Furthermore, western blotting demonstrated downregulation of protein expression of PI3K, Akt, NF-kB, Bcl-2, Cdk1 and cyclin B1, whereas Bax and caspase-3 were upregulated. Our results suggest that the extract induced cell cycle G2/M arrest and apoptosis in MDA-MB-231 cells by decreasing PI3K/Akt pathway. Therefore, we propose that ECMS has potential as a breast cancer chemotherapeutic agent.

Authors+Show Affiliations

Department of Pharmacy, Second Military Medical University, Shanghai, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22471502

Citation

Meng, Lin-Yi, et al. "Cochinchina Momordica Seed Extract Induces G2/M Arrest and Apoptosis in Human Breast Cancer MDA-MB-231 Cells By Modulating the PI3K/Akt Pathway." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 12, no. 12, 2011, pp. 3483-8.
Meng LY, Liu HR, Shen Y, et al. Cochinchina momordica seed extract induces G2/M arrest and apoptosis in human breast cancer MDA-MB-231 cells by modulating the PI3K/Akt pathway. Asian Pac J Cancer Prev. 2011;12(12):3483-8.
Meng, L. Y., Liu, H. R., Shen, Y., Yu, Y. Q., & Tao, X. (2011). Cochinchina momordica seed extract induces G2/M arrest and apoptosis in human breast cancer MDA-MB-231 cells by modulating the PI3K/Akt pathway. Asian Pacific Journal of Cancer Prevention : APJCP, 12(12), 3483-8.
Meng LY, et al. Cochinchina Momordica Seed Extract Induces G2/M Arrest and Apoptosis in Human Breast Cancer MDA-MB-231 Cells By Modulating the PI3K/Akt Pathway. Asian Pac J Cancer Prev. 2011;12(12):3483-8. PubMed PMID: 22471502.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cochinchina momordica seed extract induces G2/M arrest and apoptosis in human breast cancer MDA-MB-231 cells by modulating the PI3K/Akt pathway. AU - Meng,Lin-Yi, AU - Liu,Hong-Rui, AU - Shen,Yang, AU - Yu,Yun-Qiu, AU - Tao,Xia, PY - 2012/4/5/entrez PY - 2011/1/1/pubmed PY - 2012/8/2/medline SP - 3483 EP - 8 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac J Cancer Prev VL - 12 IS - 12 N2 - Cochinchina momordica seeds are a kind of traditional Chinese herb. In this study, anticancer activity and underlying mechanisms were investigated with an extract using human breast cancer MDA-MB-231 cells. The survival rate was reduced in a concentration- and time-dependent manner as assessed by MTT assay. After incubation for 48 h, typical apoptotic morphological changes were observed by Hoechst 33258 dye assay. Flow cytometry revealed that the treatment obviously induced G2/M arrest and apoptosis in MDA-MB-231 cells. Furthermore, western blotting demonstrated downregulation of protein expression of PI3K, Akt, NF-kB, Bcl-2, Cdk1 and cyclin B1, whereas Bax and caspase-3 were upregulated. Our results suggest that the extract induced cell cycle G2/M arrest and apoptosis in MDA-MB-231 cells by decreasing PI3K/Akt pathway. Therefore, we propose that ECMS has potential as a breast cancer chemotherapeutic agent. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/22471502/Cochinchina_momordica_seed_extract_induces_G2/M_arrest_and_apoptosis_in_human_breast_cancer_MDA_MB_231_cells_by_modulating_the_PI3K/Akt_pathway_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:22471502&key=2011.12.12.3483 DB - PRIME DP - Unbound Medicine ER -