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Evaluation of the use of nonesterified fatty acids and β-hydroxybutyrate concentrations in pooled serum samples for herd-based detection of subclinical ketosis in dairy cows during the first week after parturition.
J Am Vet Med Assoc. 2012 Apr 15; 240(8):1003-11.JA

Abstract

OBJECTIVE

To evaluate the use of nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in pooled serum samples for herd-based detection of subclinical ketosis (SCK) in dairy cows after calving.

DESIGN

Cross-sectional study.

ANIMALS

1,100 dairy cows from 110 herds.

PROCEDURES

Blood samples were collected from 10 healthy cows/herd in the first week after parturition. Aliquots of serum were mixed to create a pooled sample. Concentrations of NEFA and BHBA were measured to estimate prevalence of SCK. Pooled sample test results were compared with those obtained for individual samples. Linear regression and receiver-operating characteristic curve analysis were performed; Bland-Altman plots were used to evaluate agreement between methods.

RESULTS

Overall prevalence of SCK was 30.7%, 19.3%, and 13.6%, as determined by use of BHBA threshold concentrations of 1,000, 1,200, and 1,400 μmol/L, respectively. Pooled sample concentrations of NEFA and BHBA were significantly correlated (r = 0.98 and 0.97, respectively) with individual sample means and with the number of cows that had NEFA (R(2) range, 0.81 to 0.84) or BHBA (R(2) range, 0.65 to 0.76) concentrations above predefined thresholds. Pooled sample concentrations of NEFA and BHBA were very accurate to highly accurate for herd-based detection of SCK.

CONCLUSIONS AND CLINICAL RELEVANCE

Analysis of NEFA and BHBA concentrations in pooled serum samples was useful for herd-based detection of SCK. A sample size of 10 cows/herd was deemed adequate for monitoring dairy herds for SCK. Reference criteria specific to pooled samples should be used for this type of herd-based testing.

Authors+Show Affiliations

Clinic for Ruminant Health, Faculty of Veterinary Medicine, Freie Universität Berlin, 14163 Berlin, Germany. borchardt_vetmed@hotmail.deNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22471831

Citation

Borchardt, Stefan, and Rudolf Staufenbiel. "Evaluation of the Use of Nonesterified Fatty Acids and Β-hydroxybutyrate Concentrations in Pooled Serum Samples for Herd-based Detection of Subclinical Ketosis in Dairy Cows During the First Week After Parturition." Journal of the American Veterinary Medical Association, vol. 240, no. 8, 2012, pp. 1003-11.
Borchardt S, Staufenbiel R. Evaluation of the use of nonesterified fatty acids and β-hydroxybutyrate concentrations in pooled serum samples for herd-based detection of subclinical ketosis in dairy cows during the first week after parturition. J Am Vet Med Assoc. 2012;240(8):1003-11.
Borchardt, S., & Staufenbiel, R. (2012). Evaluation of the use of nonesterified fatty acids and β-hydroxybutyrate concentrations in pooled serum samples for herd-based detection of subclinical ketosis in dairy cows during the first week after parturition. Journal of the American Veterinary Medical Association, 240(8), 1003-11. https://doi.org/10.2460/javma.240.8.1003
Borchardt S, Staufenbiel R. Evaluation of the Use of Nonesterified Fatty Acids and Β-hydroxybutyrate Concentrations in Pooled Serum Samples for Herd-based Detection of Subclinical Ketosis in Dairy Cows During the First Week After Parturition. J Am Vet Med Assoc. 2012 Apr 15;240(8):1003-11. PubMed PMID: 22471831.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of the use of nonesterified fatty acids and β-hydroxybutyrate concentrations in pooled serum samples for herd-based detection of subclinical ketosis in dairy cows during the first week after parturition. AU - Borchardt,Stefan, AU - Staufenbiel,Rudolf, PY - 2012/4/5/entrez PY - 2012/4/5/pubmed PY - 2012/8/3/medline SP - 1003 EP - 11 JF - Journal of the American Veterinary Medical Association JO - J. Am. Vet. Med. Assoc. VL - 240 IS - 8 N2 - OBJECTIVE: To evaluate the use of nonesterified fatty acids (NEFA) and β-hydroxybutyrate (BHBA) concentrations in pooled serum samples for herd-based detection of subclinical ketosis (SCK) in dairy cows after calving. DESIGN: Cross-sectional study. ANIMALS: 1,100 dairy cows from 110 herds. PROCEDURES: Blood samples were collected from 10 healthy cows/herd in the first week after parturition. Aliquots of serum were mixed to create a pooled sample. Concentrations of NEFA and BHBA were measured to estimate prevalence of SCK. Pooled sample test results were compared with those obtained for individual samples. Linear regression and receiver-operating characteristic curve analysis were performed; Bland-Altman plots were used to evaluate agreement between methods. RESULTS: Overall prevalence of SCK was 30.7%, 19.3%, and 13.6%, as determined by use of BHBA threshold concentrations of 1,000, 1,200, and 1,400 μmol/L, respectively. Pooled sample concentrations of NEFA and BHBA were significantly correlated (r = 0.98 and 0.97, respectively) with individual sample means and with the number of cows that had NEFA (R(2) range, 0.81 to 0.84) or BHBA (R(2) range, 0.65 to 0.76) concentrations above predefined thresholds. Pooled sample concentrations of NEFA and BHBA were very accurate to highly accurate for herd-based detection of SCK. CONCLUSIONS AND CLINICAL RELEVANCE: Analysis of NEFA and BHBA concentrations in pooled serum samples was useful for herd-based detection of SCK. A sample size of 10 cows/herd was deemed adequate for monitoring dairy herds for SCK. Reference criteria specific to pooled samples should be used for this type of herd-based testing. SN - 1943-569X UR - https://www.unboundmedicine.com/medline/citation/22471831/Evaluation_of_the_use_of_nonesterified_fatty_acids_and_β_hydroxybutyrate_concentrations_in_pooled_serum_samples_for_herd_based_detection_of_subclinical_ketosis_in_dairy_cows_during_the_first_week_after_parturition_ L2 - http://avmajournals.avma.org/doi/full/10.2460/javma.240.8.1003?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -