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In vitro evaluation of Malassezia pachydermatis susceptibility to azole compounds using E-test and CLSI microdilution methods.
Med Mycol. 2012 Nov; 50(8):795-801.MM

Abstract

Dermatitis caused by Malassezia spp., one of most common skin disease in dogs, requires prolonged therapy and/or high doses of antifungal agents. In the present study, the antifungal susceptibility of M. pachydermatis to ketoconazole (KTZ), fluconazole (FLZ), itraconazole (ITZ), posaconazole (POS) and voriconazole (VOR) was evaluated in vitro using both CLSI reference broth microdilution (CLSI BMD) and E-test. A total of 62 M. pachydermatis strains from dogs with and without skin lesions were tested. M. pachydermatis strains were susceptible to ITZ, KTZ and POS using both test methods, with the highest MIC found in tests of FLZ. Essential agreement between the two methods ranged from 87.1% (VOR) to 91.9% (ITZ), and categorical agreement from 74.2% (FLZ) to 96.8% (ITZ). Minor error discrepancies were observed between the two methods, with major discrepancies observed for KTZ. A higher MIC(50) value for FLZ was noted with M. pachydermatis genotype B. The MICs(50) of M. pachydermatis genotype B for KTZ, VOR and POS were higher in isolates from dogs with skin lesions than those in isolates from animals without skin lesions. The results suggest a link between genotypes of M. pachydermatis and in vitro drug susceptibility. The categorical agreement for both E-test and CLSI BMD methods found in this investigation confirms the E-test as a reliable diagnostic method for routine use in clinical mycology laboratories.

Authors+Show Affiliations

Dipartimento di Sanità Pubblica e Zootecnia, Universit à degli Studi di Bari, Bari, Italy.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22471886

Citation

Cafarchia, Claudia, et al. "In Vitro Evaluation of Malassezia Pachydermatis Susceptibility to Azole Compounds Using E-test and CLSI Microdilution Methods." Medical Mycology, vol. 50, no. 8, 2012, pp. 795-801.
Cafarchia C, Figueredo LA, Iatta R, et al. In vitro evaluation of Malassezia pachydermatis susceptibility to azole compounds using E-test and CLSI microdilution methods. Med Mycol. 2012;50(8):795-801.
Cafarchia, C., Figueredo, L. A., Iatta, R., Colao, V., Montagna, M. T., & Otranto, D. (2012). In vitro evaluation of Malassezia pachydermatis susceptibility to azole compounds using E-test and CLSI microdilution methods. Medical Mycology, 50(8), 795-801. https://doi.org/10.3109/13693786.2012.674219
Cafarchia C, et al. In Vitro Evaluation of Malassezia Pachydermatis Susceptibility to Azole Compounds Using E-test and CLSI Microdilution Methods. Med Mycol. 2012;50(8):795-801. PubMed PMID: 22471886.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro evaluation of Malassezia pachydermatis susceptibility to azole compounds using E-test and CLSI microdilution methods. AU - Cafarchia,Claudia, AU - Figueredo,Luciana A, AU - Iatta,Roberta, AU - Colao,Valeriana, AU - Montagna,Maria T, AU - Otranto,Domenico, Y1 - 2012/04/03/ PY - 2012/4/5/entrez PY - 2012/4/5/pubmed PY - 2013/3/2/medline SP - 795 EP - 801 JF - Medical mycology JO - Med. Mycol. VL - 50 IS - 8 N2 - Dermatitis caused by Malassezia spp., one of most common skin disease in dogs, requires prolonged therapy and/or high doses of antifungal agents. In the present study, the antifungal susceptibility of M. pachydermatis to ketoconazole (KTZ), fluconazole (FLZ), itraconazole (ITZ), posaconazole (POS) and voriconazole (VOR) was evaluated in vitro using both CLSI reference broth microdilution (CLSI BMD) and E-test. A total of 62 M. pachydermatis strains from dogs with and without skin lesions were tested. M. pachydermatis strains were susceptible to ITZ, KTZ and POS using both test methods, with the highest MIC found in tests of FLZ. Essential agreement between the two methods ranged from 87.1% (VOR) to 91.9% (ITZ), and categorical agreement from 74.2% (FLZ) to 96.8% (ITZ). Minor error discrepancies were observed between the two methods, with major discrepancies observed for KTZ. A higher MIC(50) value for FLZ was noted with M. pachydermatis genotype B. The MICs(50) of M. pachydermatis genotype B for KTZ, VOR and POS were higher in isolates from dogs with skin lesions than those in isolates from animals without skin lesions. The results suggest a link between genotypes of M. pachydermatis and in vitro drug susceptibility. The categorical agreement for both E-test and CLSI BMD methods found in this investigation confirms the E-test as a reliable diagnostic method for routine use in clinical mycology laboratories. SN - 1460-2709 UR - https://www.unboundmedicine.com/medline/citation/22471886/In_vitro_evaluation_of_Malassezia_pachydermatis_susceptibility_to_azole_compounds_using_E_test_and_CLSI_microdilution_methods_ L2 - https://academic.oup.com/mmy/article-lookup/doi/10.3109/13693786.2012.674219 DB - PRIME DP - Unbound Medicine ER -