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Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats.
J Ethnopharmacol. 2012 May 07; 141(1):517-25.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

The leaves of Persea americana Mill. (Lauraceae) have been popularly used in the treatment of diabetes in countries in Latin America and Africa.

AIM OF THE STUDY

To investigate the hypoglycaemic properties and to determine the molecular mechanism by which the hydroalcoholic extract of the leaves of Persea americana reduce blood glucose levels in streptozotocin (STZ)-induced diabetes in rats via the enzymatic pathway of protein kinase B (PKB/Akt).

METHODS

The hydroalcoholic extract of the leaves of Persea americana (0.15 and 0.3g/kg/day), vehicle and metformin (0.5g/kg/day) were administered orally to STZ-diabetic rats (n=7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation and the expression of PKB in the liver and soleus muscle were determined by Western blot.

RESULTS

The hydroalcoholic extract of the leaves of Persea americana reduced blood glucose levels and improved the metabolic state of the animals. Additionally, PKB activation was observed in the liver and skeletal muscle of treated rats when compared with untreated rats.

CONCLUSION

The results indicate that the hydroalcoholic extract of the leaves of Persea americana has anti-diabetic properties and possibly acts to regulate glucose uptake in liver and muscles by way of PKB/Akt activation, restoring the intracellular energy balance.

Authors+Show Affiliations

Department of Pharmaceutical Sciences, Federal University of Pernambuco, Recife 50740-521, Brazil.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22472105

Citation

Lima, C R., et al. "Anti-diabetic Activity of Extract From Persea Americana Mill. Leaf Via the Activation of Protein Kinase B (PKB/Akt) in Streptozotocin-induced Diabetic Rats." Journal of Ethnopharmacology, vol. 141, no. 1, 2012, pp. 517-25.
Lima CR, Vasconcelos CF, Costa-Silva JH, et al. Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats. J Ethnopharmacol. 2012;141(1):517-25.
Lima, C. R., Vasconcelos, C. F., Costa-Silva, J. H., Maranhão, C. A., Costa, J., Batista, T. M., Carneiro, E. M., Soares, L. A., Ferreira, F., & Wanderley, A. G. (2012). Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats. Journal of Ethnopharmacology, 141(1), 517-25. https://doi.org/10.1016/j.jep.2012.03.026
Lima CR, et al. Anti-diabetic Activity of Extract From Persea Americana Mill. Leaf Via the Activation of Protein Kinase B (PKB/Akt) in Streptozotocin-induced Diabetic Rats. J Ethnopharmacol. 2012 May 7;141(1):517-25. PubMed PMID: 22472105.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anti-diabetic activity of extract from Persea americana Mill. leaf via the activation of protein kinase B (PKB/Akt) in streptozotocin-induced diabetic rats. AU - Lima,C R, AU - Vasconcelos,C F B, AU - Costa-Silva,J H, AU - Maranhão,C A, AU - Costa,J, AU - Batista,T M, AU - Carneiro,E M, AU - Soares,L A L, AU - Ferreira,F, AU - Wanderley,A G, Y1 - 2012/03/26/ PY - 2011/12/27/received PY - 2012/03/08/revised PY - 2012/03/17/accepted PY - 2012/4/5/entrez PY - 2012/4/5/pubmed PY - 2012/8/11/medline SP - 517 EP - 25 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: The leaves of Persea americana Mill. (Lauraceae) have been popularly used in the treatment of diabetes in countries in Latin America and Africa. AIM OF THE STUDY: To investigate the hypoglycaemic properties and to determine the molecular mechanism by which the hydroalcoholic extract of the leaves of Persea americana reduce blood glucose levels in streptozotocin (STZ)-induced diabetes in rats via the enzymatic pathway of protein kinase B (PKB/Akt). METHODS: The hydroalcoholic extract of the leaves of Persea americana (0.15 and 0.3g/kg/day), vehicle and metformin (0.5g/kg/day) were administered orally to STZ-diabetic rats (n=7/group) for 4 weeks. Changes in body weight, food and water intake, fasting glucose levels and oral glucose tolerance were evaluated. Phosphorylation and the expression of PKB in the liver and soleus muscle were determined by Western blot. RESULTS: The hydroalcoholic extract of the leaves of Persea americana reduced blood glucose levels and improved the metabolic state of the animals. Additionally, PKB activation was observed in the liver and skeletal muscle of treated rats when compared with untreated rats. CONCLUSION: The results indicate that the hydroalcoholic extract of the leaves of Persea americana has anti-diabetic properties and possibly acts to regulate glucose uptake in liver and muscles by way of PKB/Akt activation, restoring the intracellular energy balance. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22472105/Anti_diabetic_activity_of_extract_from_Persea_americana_Mill__leaf_via_the_activation_of_protein_kinase_B__PKB/Akt__in_streptozotocin_induced_diabetic_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00186-9 DB - PRIME DP - Unbound Medicine ER -