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Antithrombin effect of polyphenol-rich extracts from black chokeberry and grape seeds.
Phytother Res. 2013 Jan; 27(1):71-6.PR

Abstract

Thrombin is a serine protease that cleaves the peptide bonds in proteins located on the carboxyl side of arginine. Thrombin plays a central role in thromboembolic diseases, which are the major cause of mortality. The aim of the study was to estimate the effects of plant extracts on proteolytic properties of thrombin. Thrombin was incubated with polyphenol-rich extracts from berries of Aronia melanocarpa or seeds of Vitis vinifera (0.5, 5, 50 µg/mL) and with polyphenols ((+)-catechin, (-)-epicatechin, gallic acid, chlorogenic acid, procyanidin B1, cyanidin, cyanidin 3-glucoside, quercetin). The in vitro experiments showed that both extracts in all used concentrations inhibited proteolytic activity of thrombin observed as inhibition of thrombin-induced fibrinogen polymerization, stabilized fibrin formation, and platelet aggregation. Moreover, thrombin amidolytic activity was inhibited by polyphenols belonging to the flavonoid class. Results presented in this study indicate that polyphenol-rich extracts from berries of A. melanocarpa and seeds of V. vinifera may become promising dietary supplements in the prevention of thrombotic states.

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Authors+Show Affiliations

Bijak M
Department of General Biochemistry, Institute of Biochemistry, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. mbijak@biol.uni.lodz.pl
Saluk J
No affiliation info available
Ponczek MB
No affiliation info available
Nowak P
No affiliation info available

MeSH

AntithrombinsFibrinogenHumansPhotiniaPlant ExtractsPlatelet AggregationPolyphenolsSeedsVitis

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22473647

Citation

Bijak, Michał, et al. "Antithrombin Effect of Polyphenol-rich Extracts From Black Chokeberry and Grape Seeds." Phytotherapy Research : PTR, vol. 27, no. 1, 2013, pp. 71-6.
Bijak M, Saluk J, Ponczek MB, et al. Antithrombin effect of polyphenol-rich extracts from black chokeberry and grape seeds. Phytother Res. 2013;27(1):71-6.
Bijak, M., Saluk, J., Ponczek, M. B., & Nowak, P. (2013). Antithrombin effect of polyphenol-rich extracts from black chokeberry and grape seeds. Phytotherapy Research : PTR, 27(1), 71-6. https://doi.org/10.1002/ptr.4682
Bijak M, et al. Antithrombin Effect of Polyphenol-rich Extracts From Black Chokeberry and Grape Seeds. Phytother Res. 2013;27(1):71-6. PubMed PMID: 22473647.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antithrombin effect of polyphenol-rich extracts from black chokeberry and grape seeds. AU - Bijak,Michał, AU - Saluk,Joanna, AU - Ponczek,Michał Błażej, AU - Nowak,Paweł, Y1 - 2012/04/04/ PY - 2012/01/02/received PY - 2012/02/22/revised PY - 2012/03/02/accepted PY - 2012/4/5/entrez PY - 2012/4/5/pubmed PY - 2013/4/27/medline SP - 71 EP - 6 JF - Phytotherapy research : PTR JO - Phytother Res VL - 27 IS - 1 N2 - Thrombin is a serine protease that cleaves the peptide bonds in proteins located on the carboxyl side of arginine. Thrombin plays a central role in thromboembolic diseases, which are the major cause of mortality. The aim of the study was to estimate the effects of plant extracts on proteolytic properties of thrombin. Thrombin was incubated with polyphenol-rich extracts from berries of Aronia melanocarpa or seeds of Vitis vinifera (0.5, 5, 50 µg/mL) and with polyphenols ((+)-catechin, (-)-epicatechin, gallic acid, chlorogenic acid, procyanidin B1, cyanidin, cyanidin 3-glucoside, quercetin). The in vitro experiments showed that both extracts in all used concentrations inhibited proteolytic activity of thrombin observed as inhibition of thrombin-induced fibrinogen polymerization, stabilized fibrin formation, and platelet aggregation. Moreover, thrombin amidolytic activity was inhibited by polyphenols belonging to the flavonoid class. Results presented in this study indicate that polyphenol-rich extracts from berries of A. melanocarpa and seeds of V. vinifera may become promising dietary supplements in the prevention of thrombotic states. SN - 1099-1573 UR - https://www.unboundmedicine.com/medline/citation/22473647/Antithrombin_effect_of_polyphenol_rich_extracts_from_black_chokeberry_and_grape_seeds_ L2 - https://doi.org/10.1002/ptr.4682 DB - PRIME DP - Unbound Medicine ER -