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Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices.
Pharm Biol. 2012 Jul; 50(7):857-65.PB

Abstract

CONTEXT

Spices have been used as food adjuncts and in folklore for ages. Inhibition of key enzymes (α-amylase and α-glucosidase) involved in the digestion of starch and protection against free radicals and lipid peroxidation in pancreas could be part of the therapeutic approach towards the management of hyperglycemia and dietary phenolics have shown promising potentials.

OBJECTIVE

This study investigated and compared the inhibitory properties of aqueous extracts of some tropical spices: Xylopia aethiopica [Dun.] A. Rich (Annonaceae), Monodora myristica (Gaertn.) Dunal (Annonaceae), Syzygium aromaticum [L.] Merr. et Perry (Myrtaceae), Piper guineense Schumach. et Thonn (Piperaceae), Aframomum danielli K. Schum (Zingiberaceae) and Aframomum melegueta (Rosc.) K. Schum (Zingiberaceae) against α-amylase, α-glucosidase, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and sodium nitroprusside (SNP)-induced lipid peroxidation in rat pancreas--in vitro using different spectrophotometric method.

MATERIALS AND METHODS

Aqueous extract of the spices was prepared and the ability of the spice extracts to inhibit α-amylase, α-glucosidase, DPPH radicals and SNP-induced lipid peroxidation in rat pancreas--in vitro was investigated using various spectrophotometric methods.

RESULT

All the spice extracts inhibited α-amylase (IC(50) = 2.81-4.83 mg/mL), α-glucosidase (IC(50) = 2.02-3.52 mg/mL), DPPH radicals (EC(50) = 15.47-17.38 mg/mL) and SNP-induced lipid peroxidation (14.17-94.38%), with the highest α-amylase & α-glucosidase inhibitory actions and DPPH radical scavenging ability exhibited by X. aethiopica, A. danielli and S. aromaticum, respectively. Also, the spices possess high total phenol (0.88-1.3 mg/mL) and flavonoid (0.24-0.52 mg/mL) contents with A. melegueta having the highest total phenolic and flavonoid contents.

DISCUSSION AND CONCLUSION

The inhibitory effects of the spice extracts on α-amylase, α-glucosidase, DPPH radicals and SNP-induced lipid peroxidation in pancreas (in vitro) could be attributed to the presence of biologically active phytochemicals such as phenolics and some non-phenolic constituents of the spices. Furthermore, these spices may exert their anti-diabetic properties through the mechanism of enzyme inhibition, free radicals scavenging ability and prevention of lipid peroxidation.

Authors+Show Affiliations

Department of Biochemistry, School of Sciences, Federal University of Technology, Akure, Ondo State, Nigeria.No affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22480175

Citation

Adefegha, Stephen Adeniyi, and Ganiyu Oboh. "Inhibition of Key Enzymes Linked to Type 2 Diabetes and Sodium Nitroprusside-induced Lipid Peroxidation in Rat Pancreas By Water Extractable Phytochemicals From some Tropical Spices." Pharmaceutical Biology, vol. 50, no. 7, 2012, pp. 857-65.
Adefegha SA, Oboh G. Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices. Pharm Biol. 2012;50(7):857-65.
Adefegha, S. A., & Oboh, G. (2012). Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices. Pharmaceutical Biology, 50(7), 857-65. https://doi.org/10.3109/13880209.2011.641022
Adefegha SA, Oboh G. Inhibition of Key Enzymes Linked to Type 2 Diabetes and Sodium Nitroprusside-induced Lipid Peroxidation in Rat Pancreas By Water Extractable Phytochemicals From some Tropical Spices. Pharm Biol. 2012;50(7):857-65. PubMed PMID: 22480175.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Inhibition of key enzymes linked to type 2 diabetes and sodium nitroprusside-induced lipid peroxidation in rat pancreas by water extractable phytochemicals from some tropical spices. AU - Adefegha,Stephen Adeniyi, AU - Oboh,Ganiyu, Y1 - 2012/04/06/ PY - 2012/4/7/entrez PY - 2012/4/7/pubmed PY - 2013/4/30/medline SP - 857 EP - 65 JF - Pharmaceutical biology JO - Pharm Biol VL - 50 IS - 7 N2 - CONTEXT: Spices have been used as food adjuncts and in folklore for ages. Inhibition of key enzymes (α-amylase and α-glucosidase) involved in the digestion of starch and protection against free radicals and lipid peroxidation in pancreas could be part of the therapeutic approach towards the management of hyperglycemia and dietary phenolics have shown promising potentials. OBJECTIVE: This study investigated and compared the inhibitory properties of aqueous extracts of some tropical spices: Xylopia aethiopica [Dun.] A. Rich (Annonaceae), Monodora myristica (Gaertn.) Dunal (Annonaceae), Syzygium aromaticum [L.] Merr. et Perry (Myrtaceae), Piper guineense Schumach. et Thonn (Piperaceae), Aframomum danielli K. Schum (Zingiberaceae) and Aframomum melegueta (Rosc.) K. Schum (Zingiberaceae) against α-amylase, α-glucosidase, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals and sodium nitroprusside (SNP)-induced lipid peroxidation in rat pancreas--in vitro using different spectrophotometric method. MATERIALS AND METHODS: Aqueous extract of the spices was prepared and the ability of the spice extracts to inhibit α-amylase, α-glucosidase, DPPH radicals and SNP-induced lipid peroxidation in rat pancreas--in vitro was investigated using various spectrophotometric methods. RESULT: All the spice extracts inhibited α-amylase (IC(50) = 2.81-4.83 mg/mL), α-glucosidase (IC(50) = 2.02-3.52 mg/mL), DPPH radicals (EC(50) = 15.47-17.38 mg/mL) and SNP-induced lipid peroxidation (14.17-94.38%), with the highest α-amylase & α-glucosidase inhibitory actions and DPPH radical scavenging ability exhibited by X. aethiopica, A. danielli and S. aromaticum, respectively. Also, the spices possess high total phenol (0.88-1.3 mg/mL) and flavonoid (0.24-0.52 mg/mL) contents with A. melegueta having the highest total phenolic and flavonoid contents. DISCUSSION AND CONCLUSION: The inhibitory effects of the spice extracts on α-amylase, α-glucosidase, DPPH radicals and SNP-induced lipid peroxidation in pancreas (in vitro) could be attributed to the presence of biologically active phytochemicals such as phenolics and some non-phenolic constituents of the spices. Furthermore, these spices may exert their anti-diabetic properties through the mechanism of enzyme inhibition, free radicals scavenging ability and prevention of lipid peroxidation. SN - 1744-5116 UR - https://www.unboundmedicine.com/medline/citation/22480175/Inhibition_of_key_enzymes_linked_to_type_2_diabetes_and_sodium_nitroprusside_induced_lipid_peroxidation_in_rat_pancreas_by_water_extractable_phytochemicals_from_some_tropical_spices_ L2 - https://www.tandfonline.com/doi/full/10.3109/13880209.2011.641022 DB - PRIME DP - Unbound Medicine ER -