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Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry.
J Chromatogr A. 2012 Oct 12; 1259:179-86.JC

Abstract

After ingestion, products containing Chinese star anise (Illicium verum) contaminated or adulterated with Japanese star anise (Illicium anisatum) or other Illicium species, can cause epilepsy, hallucinations, and nausea due to the rare neurotoxic sesquiterpene dilactone anisatin that is present in Japanese star anise. Thus a rapid, simple and unambiguous method for distinguishing between the morphologically similar Chinese star anise and toxic Japanese star anise is important for food safety issues. Direct Analysis in Real Time (DART) ambient ionisation coupled with orbitrap high resolution mass spectrometry allowed the recording of mass spectra of anisatin in solid star anise fruits in seconds without any prior sample pretreatment. Spectra could be obtained in both positive ([M+NH(4)](+) at m/z 346.1496, C(15)H(24)NO(8)) and negative mode ([M-H](-) at m/z 327.1074, C(15)H(19)O(8)) and gave the same outcome provided a mass resolution of at least 27,000 is available. The anisatin signal was typically >1000 times larger in Japanese star anise than in Chinese star anise thus allowing an unequivocal qualitative determination. Herbal teas containing star anise fragments too small to be visually recognised, could be analysed by preparing a tea in 6 min and subsequently sampling ∼2 μL of tea on a glass rod. None of the 8 investigated retail teas contained significant quantities of anisatin. Spiking a complex herbal tea containing Chinese star anise with an equally concentrated tea prepared from Japanese star anise provided a linear calibration curve (R(2) ≥ 0.995) after normalising on a native constituent of Chinese star anise (standard addition method). This showed that adulteration down to 1% (w/w) is still measurable. Compared with existing PCR, TLC, GC-MS and HPLC-ESI-MS/MS procedures, the proposed DART-HRMS procedure is faster and simpler and moreover measures the actual biotoxin.

Authors+Show Affiliations

Laboratory of Organic Chemistry, Wageningen University, Dreijenplein 8, 6703 HB Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22484123

Citation

Shen, Yao, et al. "Rapid Control of Chinese Star Anise Fruits and Teas for Neurotoxic Anisatin By Direct Analysis in Real Time High Resolution Mass Spectrometry." Journal of Chromatography. A, vol. 1259, 2012, pp. 179-86.
Shen Y, van Beek TA, Claassen FW, et al. Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry. J Chromatogr A. 2012;1259:179-86.
Shen, Y., van Beek, T. A., Claassen, F. W., Zuilhof, H., Chen, B., & Nielen, M. W. (2012). Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry. Journal of Chromatography. A, 1259, 179-86. https://doi.org/10.1016/j.chroma.2012.03.058
Shen Y, et al. Rapid Control of Chinese Star Anise Fruits and Teas for Neurotoxic Anisatin By Direct Analysis in Real Time High Resolution Mass Spectrometry. J Chromatogr A. 2012 Oct 12;1259:179-86. PubMed PMID: 22484123.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Rapid control of Chinese star anise fruits and teas for neurotoxic anisatin by Direct Analysis in Real Time high resolution mass spectrometry. AU - Shen,Yao, AU - van Beek,Teris A, AU - Claassen,Frank W, AU - Zuilhof,Han, AU - Chen,Bo, AU - Nielen,Michel W F, Y1 - 2012/03/23/ PY - 2011/12/22/received PY - 2012/03/13/revised PY - 2012/03/13/accepted PY - 2012/4/10/entrez PY - 2012/4/10/pubmed PY - 2013/1/8/medline SP - 179 EP - 86 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1259 N2 - After ingestion, products containing Chinese star anise (Illicium verum) contaminated or adulterated with Japanese star anise (Illicium anisatum) or other Illicium species, can cause epilepsy, hallucinations, and nausea due to the rare neurotoxic sesquiterpene dilactone anisatin that is present in Japanese star anise. Thus a rapid, simple and unambiguous method for distinguishing between the morphologically similar Chinese star anise and toxic Japanese star anise is important for food safety issues. Direct Analysis in Real Time (DART) ambient ionisation coupled with orbitrap high resolution mass spectrometry allowed the recording of mass spectra of anisatin in solid star anise fruits in seconds without any prior sample pretreatment. Spectra could be obtained in both positive ([M+NH(4)](+) at m/z 346.1496, C(15)H(24)NO(8)) and negative mode ([M-H](-) at m/z 327.1074, C(15)H(19)O(8)) and gave the same outcome provided a mass resolution of at least 27,000 is available. The anisatin signal was typically >1000 times larger in Japanese star anise than in Chinese star anise thus allowing an unequivocal qualitative determination. Herbal teas containing star anise fragments too small to be visually recognised, could be analysed by preparing a tea in 6 min and subsequently sampling ∼2 μL of tea on a glass rod. None of the 8 investigated retail teas contained significant quantities of anisatin. Spiking a complex herbal tea containing Chinese star anise with an equally concentrated tea prepared from Japanese star anise provided a linear calibration curve (R(2) ≥ 0.995) after normalising on a native constituent of Chinese star anise (standard addition method). This showed that adulteration down to 1% (w/w) is still measurable. Compared with existing PCR, TLC, GC-MS and HPLC-ESI-MS/MS procedures, the proposed DART-HRMS procedure is faster and simpler and moreover measures the actual biotoxin. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22484123/Rapid_control_of_Chinese_star_anise_fruits_and_teas_for_neurotoxic_anisatin_by_Direct_Analysis_in_Real_Time_high_resolution_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(12)00471-2 DB - PRIME DP - Unbound Medicine ER -