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Proteomic analysis of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) pollen.
J Proteomics. 2012 May 17; 75(9):2736-44.JP

Abstract

This paper presents an analysis of Holm oak pollen proteome, together with an evaluation of the potentiality that a proteomic approach may have in the provenance variability assessment. Proteins were extracted from pollen of four Holm oak provenances, and they were analyzed by gel-based (1- and 2-DE in combination with MALDI-TOF/TOF) and gel-free (nLC-LTQ Orbitrap MS) approaches. A comparison of 1- and 2-DE protein profiles of the four provenances revealed significant differences, both qualitative and quantitative, in abundance (18 bands and 16 spots, respectively). Multivariate statistical analysis carried out on bands and spots clearly showed distinct associations between provenances, which highlight their geographical origins. A total of 100 spots selected from the 402 spots observed on 2-DE gels were identified by MALDI-TOF/TOF. Moreover, a complementary gel-free shotgun approach was performed by nLC-LTQ Orbitrap MS. The identified proteins were classified according to biological processes, and most proteins in both approaches were related to metabolism and defense/stress processes. The nLC-LTQ Orbitrap MS analysis allowed us the identification of proteins belonging to the cell wall and division, transport and translation categories. Besides providing the first reference map of Holm oak pollen, our results confirm previous studies based on morphological observations and acorn proteomic analysis. Moreover, our data support the valuable use of proteomic techniques as phylogenetic tool in plant studies.

Authors+Show Affiliations

Agricultural and Plant Biochemistry and Proteomics Research Group, Dept. of Biochemistry and Molecular Biology, University of Córdoba, Agrifood Campus of International Excellence (ceiA3) 14071 Córdoba, Spain. bb2vagaj@uco.esNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22484522

Citation

Valero Galván, José, et al. "Proteomic Analysis of Holm Oak (Quercus Ilex Subsp. Ballota [Desf.] Samp.) Pollen." Journal of Proteomics, vol. 75, no. 9, 2012, pp. 2736-44.
Valero Galván J, Valledor L, González Fernandez R, et al. Proteomic analysis of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) pollen. J Proteomics. 2012;75(9):2736-44.
Valero Galván, J., Valledor, L., González Fernandez, R., Navarro Cerrillo, R. M., & Jorrín-Novo, J. V. (2012). Proteomic analysis of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) pollen. Journal of Proteomics, 75(9), 2736-44. https://doi.org/10.1016/j.jprot.2012.03.035
Valero Galván J, et al. Proteomic Analysis of Holm Oak (Quercus Ilex Subsp. Ballota [Desf.] Samp.) Pollen. J Proteomics. 2012 May 17;75(9):2736-44. PubMed PMID: 22484522.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Proteomic analysis of Holm oak (Quercus ilex subsp. ballota [Desf.] Samp.) pollen. AU - Valero Galván,José, AU - Valledor,Luis, AU - González Fernandez,Raquel, AU - Navarro Cerrillo,Rafael M, AU - Jorrín-Novo,Jesus V, Y1 - 2012/03/30/ PY - 2011/12/13/received PY - 2012/03/21/revised PY - 2012/03/21/accepted PY - 2012/4/10/entrez PY - 2012/4/10/pubmed PY - 2012/9/11/medline SP - 2736 EP - 44 JF - Journal of proteomics JO - J Proteomics VL - 75 IS - 9 N2 - This paper presents an analysis of Holm oak pollen proteome, together with an evaluation of the potentiality that a proteomic approach may have in the provenance variability assessment. Proteins were extracted from pollen of four Holm oak provenances, and they were analyzed by gel-based (1- and 2-DE in combination with MALDI-TOF/TOF) and gel-free (nLC-LTQ Orbitrap MS) approaches. A comparison of 1- and 2-DE protein profiles of the four provenances revealed significant differences, both qualitative and quantitative, in abundance (18 bands and 16 spots, respectively). Multivariate statistical analysis carried out on bands and spots clearly showed distinct associations between provenances, which highlight their geographical origins. A total of 100 spots selected from the 402 spots observed on 2-DE gels were identified by MALDI-TOF/TOF. Moreover, a complementary gel-free shotgun approach was performed by nLC-LTQ Orbitrap MS. The identified proteins were classified according to biological processes, and most proteins in both approaches were related to metabolism and defense/stress processes. The nLC-LTQ Orbitrap MS analysis allowed us the identification of proteins belonging to the cell wall and division, transport and translation categories. Besides providing the first reference map of Holm oak pollen, our results confirm previous studies based on morphological observations and acorn proteomic analysis. Moreover, our data support the valuable use of proteomic techniques as phylogenetic tool in plant studies. SN - 1876-7737 UR - https://www.unboundmedicine.com/medline/citation/22484522/Proteomic_analysis_of_Holm_oak__Quercus_ilex_subsp__ballota_[Desf_]_Samp___pollen_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1874-3919(12)00186-8 DB - PRIME DP - Unbound Medicine ER -