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Aptamer-based highly sensitive electrochemical detection of thrombin via the amplification of graphene.
Analyst. 2012 May 21; 137(10):2415-20.A

Abstract

Herein, we successfully fabricated a highly sensitive label-free electrochemical aptasensor for thrombin based on the amplification of graphene (Gra). The excellent electrochemical probe of nickel hexacyanoferrate nanoparticles (NiHCFNPs) was introduced to form Nafion-Graphene-NiHCFNPs (Nf-Gra-NiHCFNPs) nanocomposites membrane on the gold electrode. The employment of graphene not only enhanced the surface area of the electrode with increased NiHCFNPs immobilization, but also improved the conductivity of the electrode, which further effectively improved the sensitivity of this proposed aptasensor. Subsequently, AuNPs layer was formed to immobilize the thrombin aptamer (TBA) and enhance the stability of the composite monolayer mentioned above. Then, thiol-modified TBA was assembled onto the AuNPs layer. Thereafter, hexanethiol (HT) was employed to block the possible remaining active sites. With the dual amplification of Gra and AuNPs, the resulting aptasensor exhibited good current response to target thrombin with a wide linear range extended from 1 pM to 80 nM (the detection limit was 0.3 pM). Additionally, the morphologies of bare Au substrate, nickel hexacyanoferrate nanoparticles (NiHCFNPs) and nanocomposites were successfully characterized by atomic force microscopy (AFM).

Authors+Show Affiliations

Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22489284

Citation

Jiang, Liping, et al. "Aptamer-based Highly Sensitive Electrochemical Detection of Thrombin Via the Amplification of Graphene." The Analyst, vol. 137, no. 10, 2012, pp. 2415-20.
Jiang L, Yuan R, Chai Y, et al. Aptamer-based highly sensitive electrochemical detection of thrombin via the amplification of graphene. Analyst. 2012;137(10):2415-20.
Jiang, L., Yuan, R., Chai, Y., Yuan, Y., Bai, L., & Wang, Y. (2012). Aptamer-based highly sensitive electrochemical detection of thrombin via the amplification of graphene. The Analyst, 137(10), 2415-20. https://doi.org/10.1039/c2an35255a
Jiang L, et al. Aptamer-based Highly Sensitive Electrochemical Detection of Thrombin Via the Amplification of Graphene. Analyst. 2012 May 21;137(10):2415-20. PubMed PMID: 22489284.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Aptamer-based highly sensitive electrochemical detection of thrombin via the amplification of graphene. AU - Jiang,Liping, AU - Yuan,Ruo, AU - Chai,Yaqin, AU - Yuan,Yali, AU - Bai,Lijuan, AU - Wang,Yan, Y1 - 2012/04/10/ PY - 2012/4/11/entrez PY - 2012/4/11/pubmed PY - 2012/8/16/medline SP - 2415 EP - 20 JF - The Analyst JO - Analyst VL - 137 IS - 10 N2 - Herein, we successfully fabricated a highly sensitive label-free electrochemical aptasensor for thrombin based on the amplification of graphene (Gra). The excellent electrochemical probe of nickel hexacyanoferrate nanoparticles (NiHCFNPs) was introduced to form Nafion-Graphene-NiHCFNPs (Nf-Gra-NiHCFNPs) nanocomposites membrane on the gold electrode. The employment of graphene not only enhanced the surface area of the electrode with increased NiHCFNPs immobilization, but also improved the conductivity of the electrode, which further effectively improved the sensitivity of this proposed aptasensor. Subsequently, AuNPs layer was formed to immobilize the thrombin aptamer (TBA) and enhance the stability of the composite monolayer mentioned above. Then, thiol-modified TBA was assembled onto the AuNPs layer. Thereafter, hexanethiol (HT) was employed to block the possible remaining active sites. With the dual amplification of Gra and AuNPs, the resulting aptasensor exhibited good current response to target thrombin with a wide linear range extended from 1 pM to 80 nM (the detection limit was 0.3 pM). Additionally, the morphologies of bare Au substrate, nickel hexacyanoferrate nanoparticles (NiHCFNPs) and nanocomposites were successfully characterized by atomic force microscopy (AFM). SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22489284/Aptamer_based_highly_sensitive_electrochemical_detection_of_thrombin_via_the_amplification_of_graphene_ L2 - https://doi.org/10.1039/c2an35255a DB - PRIME DP - Unbound Medicine ER -