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CD8+CD28-lymphocytes in peripheral blood and serum concentrations of soluble interleukin 6 receptor are increased in patients with Graves' orbitopathy and correlate with disease activity.
Endocr Res. 2012; 37(2):89-95.ER

Abstract

BACKGROUND

The extrathyroid, orbital manifestation of Graves' disease (GD)--Graves' orbitopathy (GO)--presents a difficult clinical problem. The immunological status of GO patients is still under investigation. The aim of this study was to assess the serum concentration of interleukin 6 (IL-6), soluble interleukin 6 receptor (sIL-6R), and CD8+CD28- lymphocytes in GO patients and to evaluate if these parameters were associated with disease activity.

PATIENTS

Thirty-nine patients (29 women and 10 men, aged 24-71, mean 50.18) with newly diagnosed GD were enrolled in the study. Active GO was diagnosed in 20 patients. The control group included 12 healthy individuals.

METHODS

Serum concentrations of IL-6 and sIL-6R were estimated by ELISA. Percentages of CD8+CD28- lymphocytes in peripheral blood were assessed by flow cytometry.

RESULTS

Mean serum IL-6 and sIL-6R concentrations were significantly higher in all GD patients and in GO and non-GO patients than in normal controls. In all GD patients and the non-GO group, serum IL-6 and sIL-6R concentrations were significantly reduced after efficient treatment. In GO patients, only serum sIL-6R concentration was significantly lower after efficient treatment. In all GD patients, the mean percentage of CD8+CD28- lymphocytes was significantly lower after efficient treatment. In GO patients, the mean percentage of CD8+CD28- lymphocytes was significantly higher than in the non-GO group or in normals. Moreover, in the GO group, the mean percentage of CD8+CD28- lymphocytes was significantly lower after treatment.

CONCLUSION

Our results have shown that CD8+CD28- lymphocyte percentage in peripheral blood and serum concentration of sIL-6R are increased in GO patients and correlate with disease activity.

Authors+Show Affiliations

Department of Ophthalmology, Wroclaw Medical University, Wroclaw, Poland. mikun11@wp.plNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22489922

Citation

Slowik, Miroslaw, et al. "CD8+CD28-lymphocytes in Peripheral Blood and Serum Concentrations of Soluble Interleukin 6 Receptor Are Increased in Patients With Graves' Orbitopathy and Correlate With Disease Activity." Endocrine Research, vol. 37, no. 2, 2012, pp. 89-95.
Slowik M, Urbaniak-Kujda D, Bohdanowicz-Pawlak A, et al. CD8+CD28-lymphocytes in peripheral blood and serum concentrations of soluble interleukin 6 receptor are increased in patients with Graves' orbitopathy and correlate with disease activity. Endocr Res. 2012;37(2):89-95.
Slowik, M., Urbaniak-Kujda, D., Bohdanowicz-Pawlak, A., Kapelko-Slowik, K., Dybko, J., Wolowiec, D., Jazwiec, B., & Daroszewski, J. (2012). CD8+CD28-lymphocytes in peripheral blood and serum concentrations of soluble interleukin 6 receptor are increased in patients with Graves' orbitopathy and correlate with disease activity. Endocrine Research, 37(2), 89-95. https://doi.org/10.3109/07435800.2011.635622
Slowik M, et al. CD8+CD28-lymphocytes in Peripheral Blood and Serum Concentrations of Soluble Interleukin 6 Receptor Are Increased in Patients With Graves' Orbitopathy and Correlate With Disease Activity. Endocr Res. 2012;37(2):89-95. PubMed PMID: 22489922.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - CD8+CD28-lymphocytes in peripheral blood and serum concentrations of soluble interleukin 6 receptor are increased in patients with Graves' orbitopathy and correlate with disease activity. AU - Slowik,Miroslaw, AU - Urbaniak-Kujda,Donata, AU - Bohdanowicz-Pawlak,Anna, AU - Kapelko-Slowik,Katarzyna, AU - Dybko,Jaroslaw, AU - Wolowiec,Dariusz, AU - Jazwiec,Bozena, AU - Daroszewski,Jacek, PY - 2012/4/12/entrez PY - 2012/4/12/pubmed PY - 2012/7/31/medline SP - 89 EP - 95 JF - Endocrine research JO - Endocr. Res. VL - 37 IS - 2 N2 - BACKGROUND: The extrathyroid, orbital manifestation of Graves' disease (GD)--Graves' orbitopathy (GO)--presents a difficult clinical problem. The immunological status of GO patients is still under investigation. The aim of this study was to assess the serum concentration of interleukin 6 (IL-6), soluble interleukin 6 receptor (sIL-6R), and CD8+CD28- lymphocytes in GO patients and to evaluate if these parameters were associated with disease activity. PATIENTS: Thirty-nine patients (29 women and 10 men, aged 24-71, mean 50.18) with newly diagnosed GD were enrolled in the study. Active GO was diagnosed in 20 patients. The control group included 12 healthy individuals. METHODS: Serum concentrations of IL-6 and sIL-6R were estimated by ELISA. Percentages of CD8+CD28- lymphocytes in peripheral blood were assessed by flow cytometry. RESULTS: Mean serum IL-6 and sIL-6R concentrations were significantly higher in all GD patients and in GO and non-GO patients than in normal controls. In all GD patients and the non-GO group, serum IL-6 and sIL-6R concentrations were significantly reduced after efficient treatment. In GO patients, only serum sIL-6R concentration was significantly lower after efficient treatment. In all GD patients, the mean percentage of CD8+CD28- lymphocytes was significantly lower after efficient treatment. In GO patients, the mean percentage of CD8+CD28- lymphocytes was significantly higher than in the non-GO group or in normals. Moreover, in the GO group, the mean percentage of CD8+CD28- lymphocytes was significantly lower after treatment. CONCLUSION: Our results have shown that CD8+CD28- lymphocyte percentage in peripheral blood and serum concentration of sIL-6R are increased in GO patients and correlate with disease activity. SN - 1532-4206 UR - https://www.unboundmedicine.com/medline/citation/22489922/CD8+CD28_lymphocytes_in_peripheral_blood_and_serum_concentrations_of_soluble_interleukin_6_receptor_are_increased_in_patients_with_Graves'_orbitopathy_and_correlate_with_disease_activity_ L2 - http://www.tandfonline.com/doi/full/10.3109/07435800.2011.635622 DB - PRIME DP - Unbound Medicine ER -