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Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population.
Rheumatology (Oxford). 2012 Aug; 51(8):1465-70.R

Abstract

OBJECTIVES

To compare the SSc-line immunoassay (LIA) with conventional techniques of antibody detection, to evaluate its diagnostic utility and to describe clinical associations of antibodies in Asian SSc patients.

METHODS

Stored sera from patients with SSc (n = 68), SLE (n = 49), OA (n = 41) and normal controls (NCs, n = 32) were evaluated. Cohen's κ and Bland-Altman plots were used to evaluate agreement.

RESULTS

There was good agreement between LIA and ELISA for anti-Scl-70 (κ = 0.97), anti-CENPA (κ = 0.83), anti-CENPB (κ = 0.96) and anti-PmScl100 (κ = 1.00) (5.48-8.22% of values outside the 95% limits of agreement using Bland-Altman plots), and between LIA and IIF for anti-CENPA (κ = 0.81) and anti-CENPB (κ = 0.77) (P < 0.001). Using LIA, of 32 (32/68, 47%) SSc patients negative for anti-Scl-70 and anti-CENPA/B, 5 (5/32, 15%) were positive for anti-Ku, -Nor90, -fibrillarin and -RP155. Specificity of each antibody for SSc was at least 97% (vs OA/NC) and 94% (vs SLE), except for anti-Ro52 (63%). Anti-CENPB was associated with joint pain [odds ratio (OR) 0.17], interstitial lung disease (OR 0.24) and telangiectasia (OR 4.00) (P < 0.05). Anti-Ro60 was associated with pulmonary arterial hypertension (OR 3.89, P = 0.041).

CONCLUSION

The SSc-LIA has good agreement with conventional techniques for selected antibodies and has good diagnostic utility.

Authors+Show Affiliations

Block 6 Level 9, Department of Rheumatology and Immunology, Singapore General Hospital, Outram Road, Singapore 169608. andrea.low.h.l@sgh.com.sgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22491717

Citation

Low, Andrea H L., et al. "Evaluation of a New Multi-parallel Line Immunoassay for Systemic Sclerosis-associated Antibodies in an Asian Population." Rheumatology (Oxford, England), vol. 51, no. 8, 2012, pp. 1465-70.
Low AH, Wong S, Thumboo J, et al. Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population. Rheumatology (Oxford). 2012;51(8):1465-70.
Low, A. H., Wong, S., Thumboo, J., Ng, S. C., Lim, J. Y., Ng, X., Earnest, A., & Fong, K. Y. (2012). Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population. Rheumatology (Oxford, England), 51(8), 1465-70. https://doi.org/10.1093/rheumatology/kes055
Low AH, et al. Evaluation of a New Multi-parallel Line Immunoassay for Systemic Sclerosis-associated Antibodies in an Asian Population. Rheumatology (Oxford). 2012;51(8):1465-70. PubMed PMID: 22491717.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of a new multi-parallel line immunoassay for systemic sclerosis-associated antibodies in an Asian population. AU - Low,Andrea H L, AU - Wong,Stephen, AU - Thumboo,Julian, AU - Ng,Swee Cheng, AU - Lim,Jia Yuen, AU - Ng,Xinyi, AU - Earnest,Arul, AU - Fong,Kok Yong, Y1 - 2012/04/06/ PY - 2012/4/12/entrez PY - 2012/4/12/pubmed PY - 2012/10/4/medline SP - 1465 EP - 70 JF - Rheumatology (Oxford, England) JO - Rheumatology (Oxford) VL - 51 IS - 8 N2 - OBJECTIVES: To compare the SSc-line immunoassay (LIA) with conventional techniques of antibody detection, to evaluate its diagnostic utility and to describe clinical associations of antibodies in Asian SSc patients. METHODS: Stored sera from patients with SSc (n = 68), SLE (n = 49), OA (n = 41) and normal controls (NCs, n = 32) were evaluated. Cohen's κ and Bland-Altman plots were used to evaluate agreement. RESULTS: There was good agreement between LIA and ELISA for anti-Scl-70 (κ = 0.97), anti-CENPA (κ = 0.83), anti-CENPB (κ = 0.96) and anti-PmScl100 (κ = 1.00) (5.48-8.22% of values outside the 95% limits of agreement using Bland-Altman plots), and between LIA and IIF for anti-CENPA (κ = 0.81) and anti-CENPB (κ = 0.77) (P < 0.001). Using LIA, of 32 (32/68, 47%) SSc patients negative for anti-Scl-70 and anti-CENPA/B, 5 (5/32, 15%) were positive for anti-Ku, -Nor90, -fibrillarin and -RP155. Specificity of each antibody for SSc was at least 97% (vs OA/NC) and 94% (vs SLE), except for anti-Ro52 (63%). Anti-CENPB was associated with joint pain [odds ratio (OR) 0.17], interstitial lung disease (OR 0.24) and telangiectasia (OR 4.00) (P < 0.05). Anti-Ro60 was associated with pulmonary arterial hypertension (OR 3.89, P = 0.041). CONCLUSION: The SSc-LIA has good agreement with conventional techniques for selected antibodies and has good diagnostic utility. SN - 1462-0332 UR - https://www.unboundmedicine.com/medline/citation/22491717/Evaluation_of_a_new_multi_parallel_line_immunoassay_for_systemic_sclerosis_associated_antibodies_in_an_Asian_population_ L2 - https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/kes055 DB - PRIME DP - Unbound Medicine ER -