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The role of the anteriolateral bed nucleus of the stria terminalis in stress-induced nociception.

Abstract

Activation of the central amygdala (CeA) by corticosterone (CORT) induces somatic and colonic hypersensitivity through corticotrophin-releasing factor (CRF)-dependent mechanisms. However, the importance of the bed nucleus of the stria terminalis (BNST), part of the extended amygdala, on nociception remains unexplored. In the present study, we test the hypothesis that stimulation of the CeA by CORT induces somatic and colonic hypersensitivity through activation of the anteriolateral BNST (BNST(AL)). Animals were implanted with micropellets of CORT or cholesterol (CHOL) onto the CeA or the BNST(AL). Mechanical sensitivity was quantified using electronic von Frey filaments, and colonic nociception was measured by quantifying a visceromotor response to graded colorectal distension. In situ hybridization was used to determine mRNA levels for CRF, CRF(1), and CRF(2) receptors in the BNST(AL). In a second group, animals were implanted bilaterally with 1) CORT or CHOL micropellets onto the CeA; and 2) cannulas localized to the BNST(AL) to administer a CRF(1) receptor antagonist (CP376395). Animals implanted with CORT onto the CeA, but not the BNST(AL), exhibited increased expression of CRF mRNA and increased CRF(1)-to-CRF(2) receptor ratio in the BNST, as well as somatic and colonic hypersensitivity compared with CHOL controls. Infusion of CP376395 into the BNST(AL) inhibited somatic and colonic hypersensitivity in response to elevated amygdala CORT. Somatic and colonic hypersensitivity induced by elevated amygdala CORT is mediated via a CRF(1) receptor-dependent mechanism in the BNST(AL). The CeA through a descending pathway involving the BNST(AL) plays a pivotal role in somatic and colonic nociception.

Authors+Show Affiliations

VA Medical Center, Oklahoma City, OK 73104, USA.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22492693

Citation

Tran, Lee, et al. "The Role of the Anteriolateral Bed Nucleus of the Stria Terminalis in Stress-induced Nociception." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 302, no. 11, 2012, pp. G1301-9.
Tran L, Wiskur B, Greenwood-Van Meerveld B. The role of the anteriolateral bed nucleus of the stria terminalis in stress-induced nociception. Am J Physiol Gastrointest Liver Physiol. 2012;302(11):G1301-9.
Tran, L., Wiskur, B., & Greenwood-Van Meerveld, B. (2012). The role of the anteriolateral bed nucleus of the stria terminalis in stress-induced nociception. American Journal of Physiology. Gastrointestinal and Liver Physiology, 302(11), pp. G1301-9. doi:10.1152/ajpgi.00501.2011.
Tran L, Wiskur B, Greenwood-Van Meerveld B. The Role of the Anteriolateral Bed Nucleus of the Stria Terminalis in Stress-induced Nociception. Am J Physiol Gastrointest Liver Physiol. 2012 Jun 1;302(11):G1301-9. PubMed PMID: 22492693.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The role of the anteriolateral bed nucleus of the stria terminalis in stress-induced nociception. AU - Tran,Lee, AU - Wiskur,Brandt, AU - Greenwood-Van Meerveld,Beverley, Y1 - 2012/04/05/ PY - 2012/4/12/entrez PY - 2012/4/12/pubmed PY - 2012/8/11/medline SP - G1301 EP - 9 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am. J. Physiol. Gastrointest. Liver Physiol. VL - 302 IS - 11 N2 - Activation of the central amygdala (CeA) by corticosterone (CORT) induces somatic and colonic hypersensitivity through corticotrophin-releasing factor (CRF)-dependent mechanisms. However, the importance of the bed nucleus of the stria terminalis (BNST), part of the extended amygdala, on nociception remains unexplored. In the present study, we test the hypothesis that stimulation of the CeA by CORT induces somatic and colonic hypersensitivity through activation of the anteriolateral BNST (BNST(AL)). Animals were implanted with micropellets of CORT or cholesterol (CHOL) onto the CeA or the BNST(AL). Mechanical sensitivity was quantified using electronic von Frey filaments, and colonic nociception was measured by quantifying a visceromotor response to graded colorectal distension. In situ hybridization was used to determine mRNA levels for CRF, CRF(1), and CRF(2) receptors in the BNST(AL). In a second group, animals were implanted bilaterally with 1) CORT or CHOL micropellets onto the CeA; and 2) cannulas localized to the BNST(AL) to administer a CRF(1) receptor antagonist (CP376395). Animals implanted with CORT onto the CeA, but not the BNST(AL), exhibited increased expression of CRF mRNA and increased CRF(1)-to-CRF(2) receptor ratio in the BNST, as well as somatic and colonic hypersensitivity compared with CHOL controls. Infusion of CP376395 into the BNST(AL) inhibited somatic and colonic hypersensitivity in response to elevated amygdala CORT. Somatic and colonic hypersensitivity induced by elevated amygdala CORT is mediated via a CRF(1) receptor-dependent mechanism in the BNST(AL). The CeA through a descending pathway involving the BNST(AL) plays a pivotal role in somatic and colonic nociception. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/22492693/The_role_of_the_anteriolateral_bed_nucleus_of_the_stria_terminalis_in_stress_induced_nociception_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.00501.2011?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -