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Transplantable rat choriocarcinoma cells express placental lactogen: identification of placental lactogen-I immunoreactive protein and messenger ribonucleic acid.
Endocrinology. 1990 Dec; 127(6):3131-7.E

Abstract

The purpose of this investigation was to evaluate the abilities of a transplantable rat choriocarcinoma (Rcho) to produce placental PRLs. The Rcho tumor was analyzed biochemically and histologically for the expression of placental PRLs. Expression of placental PRL mRNAs was determined by Northern blot and in situ hybridization analyses. Expression of placental PRL proteins was determined by Western blot and immunocytochemical analyses. Histologically, Rcho tumors were characterized by the appearance of giant cell surrounding hemorrhagic regions. Female rats bearing the Rcho tumor beneath their kidney capsule showed extensive mammary gland development. The Rcho tumors expressed placental lactogen-I (PL-I) mRNA and protein, but there was no evidence of placental lactogen-II (PL-II), PRL-like protein-A (PLP-A), or PRL-like protein-B (PLP-B). Rcho PL-I mRNA and proteins migrated as a 1-kilobase species and a 36- to 40-kDa species similar to those expressed by normal rat trophoblast tissues. The cell type responsible for Rcho PL-I production was the giant cell, similar to that observed in normal rat trophoblast tissues. In summary, we have demonstrated the production of PL-I by a transplantable rat choriocarcinoma (Rcho). The Rcho tumor resembles rat trophoblast tissue at early postimplantation stages (days 6-10 of gestation) and may be a useful tool for studying placental PRL expression during trophoblast differentiation.

Authors+Show Affiliations

Faria TN
Department of Physiology, University of Kansas Medical Center, Kansas City 66103.
Deb S
No affiliation info available
Kwok SC
No affiliation info available
Vandeputte M
No affiliation info available
Talamantes F
No affiliation info available
Soares MJ
No affiliation info available

MeSH

AnimalsBlotting, NorthernBlotting, WesternCell LineChoriocarcinomaFemaleNeoplasm TransplantationPlacental LactogenPregnancyRNA, MessengerRatsRats, Inbred LewRestriction MappingUterine Neoplasms

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

2249643

Citation

Faria, T N., et al. "Transplantable Rat Choriocarcinoma Cells Express Placental Lactogen: Identification of Placental lactogen-I Immunoreactive Protein and Messenger Ribonucleic Acid." Endocrinology, vol. 127, no. 6, 1990, pp. 3131-7.
Faria TN, Deb S, Kwok SC, et al. Transplantable rat choriocarcinoma cells express placental lactogen: identification of placental lactogen-I immunoreactive protein and messenger ribonucleic acid. Endocrinology. 1990;127(6):3131-7.
Faria, T. N., Deb, S., Kwok, S. C., Vandeputte, M., Talamantes, F., & Soares, M. J. (1990). Transplantable rat choriocarcinoma cells express placental lactogen: identification of placental lactogen-I immunoreactive protein and messenger ribonucleic acid. Endocrinology, 127(6), 3131-7.
Faria TN, et al. Transplantable Rat Choriocarcinoma Cells Express Placental Lactogen: Identification of Placental lactogen-I Immunoreactive Protein and Messenger Ribonucleic Acid. Endocrinology. 1990;127(6):3131-7. PubMed PMID: 2249643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Transplantable rat choriocarcinoma cells express placental lactogen: identification of placental lactogen-I immunoreactive protein and messenger ribonucleic acid. AU - Faria,T N, AU - Deb,S, AU - Kwok,S C, AU - Vandeputte,M, AU - Talamantes,F, AU - Soares,M J, PY - 1990/12/1/pubmed PY - 1990/12/1/medline PY - 1990/12/1/entrez SP - 3131 EP - 7 JF - Endocrinology JO - Endocrinology VL - 127 IS - 6 N2 - The purpose of this investigation was to evaluate the abilities of a transplantable rat choriocarcinoma (Rcho) to produce placental PRLs. The Rcho tumor was analyzed biochemically and histologically for the expression of placental PRLs. Expression of placental PRL mRNAs was determined by Northern blot and in situ hybridization analyses. Expression of placental PRL proteins was determined by Western blot and immunocytochemical analyses. Histologically, Rcho tumors were characterized by the appearance of giant cell surrounding hemorrhagic regions. Female rats bearing the Rcho tumor beneath their kidney capsule showed extensive mammary gland development. The Rcho tumors expressed placental lactogen-I (PL-I) mRNA and protein, but there was no evidence of placental lactogen-II (PL-II), PRL-like protein-A (PLP-A), or PRL-like protein-B (PLP-B). Rcho PL-I mRNA and proteins migrated as a 1-kilobase species and a 36- to 40-kDa species similar to those expressed by normal rat trophoblast tissues. The cell type responsible for Rcho PL-I production was the giant cell, similar to that observed in normal rat trophoblast tissues. In summary, we have demonstrated the production of PL-I by a transplantable rat choriocarcinoma (Rcho). The Rcho tumor resembles rat trophoblast tissue at early postimplantation stages (days 6-10 of gestation) and may be a useful tool for studying placental PRL expression during trophoblast differentiation. SN - 0013-7227 UR - https://www.unboundmedicine.com/medline/citation/2249643/Transplantable_rat_choriocarcinoma_cells_express_placental_lactogen:_identification_of_placental_lactogen_I_immunoreactive_protein_and_messenger_ribonucleic_acid_ DB - PRIME DP - Unbound Medicine ER -