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Bovine haptoglobin as an adipokine: serum concentrations and tissue expression in dairy cows receiving a conjugated linoleic acids supplement throughout lactation.
Vet Immunol Immunopathol. 2012 May 15; 146(3-4):201-11.VI

Abstract

The present study aimed to characterize serum haptoglobin (Hp) concentrations throughout an entire lactation period in both primi- and multiparous cows and to compare them to the Hp mRNA expression in liver and - in view of Hp being potentially an adipokine - also in different subcutaneous (s.c.) and visceral fat depots. In addition, potential anti-inflammatory effects of long-term supplementation with conjugated linoleic acids (CLA) were evaluated by assessing Hp. Trial 1 comprised 33 cows and 16 Holstein heifers from day 21 ante partum until day 252 postpartum. The animals received 100 or 50 g/day CLA or a control fat supplement. Blood samples and biopsy (tail head fat and liver) samples were collected. Trial 2 included 25 Holstein heifers, 5 animals were slaughtered on the day of parturition, the remaining animals were allocated to either CLA (100 g/day, n=10) or control fat supplement (n=10) and slaughtered on days 42 and 105 postpartum, respectively. At slaughter, fat samples were collected from 3 different visceral depots, 3 s.c. depots and from liver tissue. Results indicated no effects of CLA on serum Hp and liver Hp mRNA for both trials and on Hp mRNA in biopsies from s.c. tail head fat. In omental and s.c. withers fat from trial 2, CLA reduced Hp mRNA on both day 42 and day 105. Hp mRNA was detectable in fat tissues from both trials with abundance values being significantly lower than in liver. The Hp mRNA abundance in the s.c. fat depots was generally higher than in the visceral depots. Haptoglobin mRNA abundance in the different tissues from trial 2 was correlated whereby all s.c. depots were interrelated. The evidence of Hp mRNA expression in adipose tissues and the presence of Hp-immune staining in histological fat sections confirm that Hp can be classified as a bovine adipokine. The lack of an evident relationship between circulating Hp concentrations and normal body fat portions in dairy cattle demonstrates that varying degrees of adiposity are not confounding factors when using Hp as inflammatory marker. The physiological changes in serum Hp concentration seem to be limited to parity and parturition. In view of the lack of effects of CLA on serum Hp concentrations, the observed reaction in two out of six different fat depots seems of marginal importance for the organisms as an entity.

Authors+Show Affiliations

Institute of Animal Science, Physiology & Hygiene Unit, University of Bonn, Katzenburgweg 7-9, 53115 Bonn, Germany.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Randomized Controlled Trial
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22498004

Citation

Saremi, B, et al. "Bovine Haptoglobin as an Adipokine: Serum Concentrations and Tissue Expression in Dairy Cows Receiving a Conjugated Linoleic Acids Supplement Throughout Lactation." Veterinary Immunology and Immunopathology, vol. 146, no. 3-4, 2012, pp. 201-11.
Saremi B, Al-Dawood A, Winand S, et al. Bovine haptoglobin as an adipokine: serum concentrations and tissue expression in dairy cows receiving a conjugated linoleic acids supplement throughout lactation. Vet Immunol Immunopathol. 2012;146(3-4):201-11.
Saremi, B., Al-Dawood, A., Winand, S., Müller, U., Pappritz, J., von Soosten, D., Rehage, J., Dänicke, S., Häussler, S., Mielenz, M., & Sauerwein, H. (2012). Bovine haptoglobin as an adipokine: serum concentrations and tissue expression in dairy cows receiving a conjugated linoleic acids supplement throughout lactation. Veterinary Immunology and Immunopathology, 146(3-4), 201-11. https://doi.org/10.1016/j.vetimm.2012.03.011
Saremi B, et al. Bovine Haptoglobin as an Adipokine: Serum Concentrations and Tissue Expression in Dairy Cows Receiving a Conjugated Linoleic Acids Supplement Throughout Lactation. Vet Immunol Immunopathol. 2012 May 15;146(3-4):201-11. PubMed PMID: 22498004.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bovine haptoglobin as an adipokine: serum concentrations and tissue expression in dairy cows receiving a conjugated linoleic acids supplement throughout lactation. AU - Saremi,B, AU - Al-Dawood,A, AU - Winand,S, AU - Müller,U, AU - Pappritz,J, AU - von Soosten,D, AU - Rehage,J, AU - Dänicke,S, AU - Häussler,S, AU - Mielenz,M, AU - Sauerwein,H, Y1 - 2012/03/29/ PY - 2011/09/09/received PY - 2012/02/10/revised PY - 2012/03/21/accepted PY - 2012/4/14/entrez PY - 2012/4/14/pubmed PY - 2012/9/20/medline SP - 201 EP - 11 JF - Veterinary immunology and immunopathology JO - Vet Immunol Immunopathol VL - 146 IS - 3-4 N2 - The present study aimed to characterize serum haptoglobin (Hp) concentrations throughout an entire lactation period in both primi- and multiparous cows and to compare them to the Hp mRNA expression in liver and - in view of Hp being potentially an adipokine - also in different subcutaneous (s.c.) and visceral fat depots. In addition, potential anti-inflammatory effects of long-term supplementation with conjugated linoleic acids (CLA) were evaluated by assessing Hp. Trial 1 comprised 33 cows and 16 Holstein heifers from day 21 ante partum until day 252 postpartum. The animals received 100 or 50 g/day CLA or a control fat supplement. Blood samples and biopsy (tail head fat and liver) samples were collected. Trial 2 included 25 Holstein heifers, 5 animals were slaughtered on the day of parturition, the remaining animals were allocated to either CLA (100 g/day, n=10) or control fat supplement (n=10) and slaughtered on days 42 and 105 postpartum, respectively. At slaughter, fat samples were collected from 3 different visceral depots, 3 s.c. depots and from liver tissue. Results indicated no effects of CLA on serum Hp and liver Hp mRNA for both trials and on Hp mRNA in biopsies from s.c. tail head fat. In omental and s.c. withers fat from trial 2, CLA reduced Hp mRNA on both day 42 and day 105. Hp mRNA was detectable in fat tissues from both trials with abundance values being significantly lower than in liver. The Hp mRNA abundance in the s.c. fat depots was generally higher than in the visceral depots. Haptoglobin mRNA abundance in the different tissues from trial 2 was correlated whereby all s.c. depots were interrelated. The evidence of Hp mRNA expression in adipose tissues and the presence of Hp-immune staining in histological fat sections confirm that Hp can be classified as a bovine adipokine. The lack of an evident relationship between circulating Hp concentrations and normal body fat portions in dairy cattle demonstrates that varying degrees of adiposity are not confounding factors when using Hp as inflammatory marker. The physiological changes in serum Hp concentration seem to be limited to parity and parturition. In view of the lack of effects of CLA on serum Hp concentrations, the observed reaction in two out of six different fat depots seems of marginal importance for the organisms as an entity. SN - 1873-2534 UR - https://www.unboundmedicine.com/medline/citation/22498004/Bovine_haptoglobin_as_an_adipokine:_serum_concentrations_and_tissue_expression_in_dairy_cows_receiving_a_conjugated_linoleic_acids_supplement_throughout_lactation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2427(12)00084-0 DB - PRIME DP - Unbound Medicine ER -