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Human umbilical cord blood-derived mesenchymal stem cell therapy promotes functional recovery of contused rat spinal cord through enhancement of endogenous cell proliferation and oligogenesis.
J Biomed Biotechnol 2012; 2012:362473JB

Abstract

Numerous studies have shown the benefits of mesenchymal stem cells (MSCs) on the repair of spinal cord injury (SCI) model and on behavioral improvement, but the underlying mechanisms remain unclear. In this study, to investigate possible mechanisms by which MSCs contribute to the alleviation of neurologic deficits, we examined the potential effect of human umbilical cord blood-derived MSCs (hUCB-MSCs) on the endogenous cell proliferation and oligogenesis after SCI. SCI was injured by contusion using a weight-drop impactor and hUCB-MSCs were transplanted into the boundary zone of the injured site. Animals received a daily injection of bromodeoxyuridine (BrdU) for 7 days after treatment to identity newly synthesized cells of ependymal and periependymal cells that immunohistochemically resembled stem/progenitor cells was evident. Behavior analysis revealed that locomotor functions of hUCB-MSCs group were restored significantly and the cavity volume was smaller in the MSCs-transplanted rats compared to the control group. In MSCs-transplanted group, TUNEL-positive cells were decreased and BrdU-positive cells were significantly increased rats compared with control group. In addition, more of BrdU-positive cells expressed neural stem/progenitor cell nestin and oligo-lineage cell such as NG2, CNPase, MBP and glial fibrillary acidic protein typical of astrocytes in the MSC-transplanted rats. Thus, endogenous cell proliferation and oligogenesis contribute to MSC-promoted functional recovery following SCI.

Authors+Show Affiliations

Institute of Catholic Integrative Medicine (ICIM), Incheon St. Mary's Hospital, The Catholic University of Korea, Incheon, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22500090

Citation

Park, Sang In, et al. "Human Umbilical Cord Blood-derived Mesenchymal Stem Cell Therapy Promotes Functional Recovery of Contused Rat Spinal Cord Through Enhancement of Endogenous Cell Proliferation and Oligogenesis." Journal of Biomedicine & Biotechnology, vol. 2012, 2012, p. 362473.
Park SI, Lim JY, Jeong CH, et al. Human umbilical cord blood-derived mesenchymal stem cell therapy promotes functional recovery of contused rat spinal cord through enhancement of endogenous cell proliferation and oligogenesis. J Biomed Biotechnol. 2012;2012:362473.
Park, S. I., Lim, J. Y., Jeong, C. H., Kim, S. M., Jun, J. A., Jeun, S. S., & Oh, W. I. (2012). Human umbilical cord blood-derived mesenchymal stem cell therapy promotes functional recovery of contused rat spinal cord through enhancement of endogenous cell proliferation and oligogenesis. Journal of Biomedicine & Biotechnology, 2012, p. 362473. doi:10.1155/2012/362473.
Park SI, et al. Human Umbilical Cord Blood-derived Mesenchymal Stem Cell Therapy Promotes Functional Recovery of Contused Rat Spinal Cord Through Enhancement of Endogenous Cell Proliferation and Oligogenesis. J Biomed Biotechnol. 2012;2012:362473. PubMed PMID: 22500090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Human umbilical cord blood-derived mesenchymal stem cell therapy promotes functional recovery of contused rat spinal cord through enhancement of endogenous cell proliferation and oligogenesis. AU - Park,Sang In, AU - Lim,Jung Yeon, AU - Jeong,Chang Hyun, AU - Kim,Seong Muk, AU - Jun,Jin Ae, AU - Jeun,Sin-Soo, AU - Oh,Won Il, Y1 - 2012/02/13/ PY - 2011/06/23/received PY - 2011/09/29/accepted PY - 2012/4/14/entrez PY - 2012/4/14/pubmed PY - 2012/9/8/medline SP - 362473 EP - 362473 JF - Journal of biomedicine & biotechnology JO - J. Biomed. Biotechnol. VL - 2012 N2 - Numerous studies have shown the benefits of mesenchymal stem cells (MSCs) on the repair of spinal cord injury (SCI) model and on behavioral improvement, but the underlying mechanisms remain unclear. In this study, to investigate possible mechanisms by which MSCs contribute to the alleviation of neurologic deficits, we examined the potential effect of human umbilical cord blood-derived MSCs (hUCB-MSCs) on the endogenous cell proliferation and oligogenesis after SCI. SCI was injured by contusion using a weight-drop impactor and hUCB-MSCs were transplanted into the boundary zone of the injured site. Animals received a daily injection of bromodeoxyuridine (BrdU) for 7 days after treatment to identity newly synthesized cells of ependymal and periependymal cells that immunohistochemically resembled stem/progenitor cells was evident. Behavior analysis revealed that locomotor functions of hUCB-MSCs group were restored significantly and the cavity volume was smaller in the MSCs-transplanted rats compared to the control group. In MSCs-transplanted group, TUNEL-positive cells were decreased and BrdU-positive cells were significantly increased rats compared with control group. In addition, more of BrdU-positive cells expressed neural stem/progenitor cell nestin and oligo-lineage cell such as NG2, CNPase, MBP and glial fibrillary acidic protein typical of astrocytes in the MSC-transplanted rats. Thus, endogenous cell proliferation and oligogenesis contribute to MSC-promoted functional recovery following SCI. SN - 1110-7251 UR - https://www.unboundmedicine.com/medline/citation/22500090/Human_umbilical_cord_blood_derived_mesenchymal_stem_cell_therapy_promotes_functional_recovery_of_contused_rat_spinal_cord_through_enhancement_of_endogenous_cell_proliferation_and_oligogenesis_ L2 - https://dx.doi.org/10.1155/2012/362473 DB - PRIME DP - Unbound Medicine ER -