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Changes in the expression of major histocompatibility complex class II antigens in liver allograft rejection.
J Pathol. 1990 Oct; 162(2):165-71.JP

Abstract

The expression of major histocompatibility complex (MHC) class II antigens was studied in human liver grafts by immunohistochemical staining with monoclonal antibodies to HLA-DR, HLA-DP, and HLA-DQ antigens. Staining was carried out on frozen sections from 13 normal livers, used as controls, and 85 post-transplant specimens in six histological categories: acute rejection (n = 25); chronic rejection (n = 21); massive haemorrhagic necrosis (n = 2); resolving acute rejection (n = 10); non-rejection complications--pure cholestasis, ischaemia, biliary obstruction (n = 23); and stable graft function greater than 1 year post-transplantation (n = 4). Staining was graded semi-quantitatively on a scale of 0-3+ in bile ducts, hepatocytes, and vascular endothelium. Expression of class II antigens was increased in bile ducts, hepatocytes, and vascular endothelium in all of the post-transplant groups compared with controls. The degree of expression of HLA-DR and HLA-DP in bile ducts and vascular endothelium was significantly greater in cases of rejection than in the non-rejection groups. These observations suggest that increased class II antigen expression may be important in the pathogenesis of immune-mediated bile duct and endothelial damage in liver allografts. Immunohistochemical staining for class II antigens in post-transplant biopsies may also be useful as an adjunct to conventional histological diagnosis.

Authors+Show Affiliations

Department of Pathology, Medical School, University of Birmingham, U.K.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

2250195

Citation

Hubscher, S G., et al. "Changes in the Expression of Major Histocompatibility Complex Class II Antigens in Liver Allograft Rejection." The Journal of Pathology, vol. 162, no. 2, 1990, pp. 165-71.
Hubscher SG, Adams DH, Elias E. Changes in the expression of major histocompatibility complex class II antigens in liver allograft rejection. J Pathol. 1990;162(2):165-71.
Hubscher, S. G., Adams, D. H., & Elias, E. (1990). Changes in the expression of major histocompatibility complex class II antigens in liver allograft rejection. The Journal of Pathology, 162(2), 165-71.
Hubscher SG, Adams DH, Elias E. Changes in the Expression of Major Histocompatibility Complex Class II Antigens in Liver Allograft Rejection. J Pathol. 1990;162(2):165-71. PubMed PMID: 2250195.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Changes in the expression of major histocompatibility complex class II antigens in liver allograft rejection. AU - Hubscher,S G, AU - Adams,D H, AU - Elias,E, PY - 1990/10/1/pubmed PY - 1990/10/1/medline PY - 1990/10/1/entrez SP - 165 EP - 71 JF - The Journal of pathology JO - J. Pathol. VL - 162 IS - 2 N2 - The expression of major histocompatibility complex (MHC) class II antigens was studied in human liver grafts by immunohistochemical staining with monoclonal antibodies to HLA-DR, HLA-DP, and HLA-DQ antigens. Staining was carried out on frozen sections from 13 normal livers, used as controls, and 85 post-transplant specimens in six histological categories: acute rejection (n = 25); chronic rejection (n = 21); massive haemorrhagic necrosis (n = 2); resolving acute rejection (n = 10); non-rejection complications--pure cholestasis, ischaemia, biliary obstruction (n = 23); and stable graft function greater than 1 year post-transplantation (n = 4). Staining was graded semi-quantitatively on a scale of 0-3+ in bile ducts, hepatocytes, and vascular endothelium. Expression of class II antigens was increased in bile ducts, hepatocytes, and vascular endothelium in all of the post-transplant groups compared with controls. The degree of expression of HLA-DR and HLA-DP in bile ducts and vascular endothelium was significantly greater in cases of rejection than in the non-rejection groups. These observations suggest that increased class II antigen expression may be important in the pathogenesis of immune-mediated bile duct and endothelial damage in liver allografts. Immunohistochemical staining for class II antigens in post-transplant biopsies may also be useful as an adjunct to conventional histological diagnosis. SN - 0022-3417 UR - https://www.unboundmedicine.com/medline/citation/2250195/Changes_in_the_expression_of_major_histocompatibility_complex_class_II_antigens_in_liver_allograft_rejection_ L2 - https://doi.org/10.1002/path.1711620210 DB - PRIME DP - Unbound Medicine ER -