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Roles of transcriptional corepressor RIP140 and coactivator PGC-1α in energy state of chronically infarcted rat hearts and mitochondrial function of cardiomyocytes.
Mol Cell Endocrinol. 2012 Oct 15; 362(1-2):11-8.MC

Abstract

Transcriptional coactivator PPARγ coactivator-1α (PGC-1α) and corepressor receptor-interacting protein 140 (RIP140) are opposing-functional regulators in maintaining energy balance of most metabolic tissues and cells. However, the relative contributions of both factors to energy metabolism in cardiomyocytes remain largely unknown. Herein, we reported that the relative protein levels of RIP140/PGC-1α were up-regulated in the failing hearts after chronic myocardial infarction (MI), and correlated negatively with the energy state index phosphocreatine (PCr)/ATP ratios. Real-time PCR analysis revealed that mRNA expressions of estrogen related receptor α (ERRα), peroxisome proliferate activated receptor α and β (PPARα, PPARβ), nuclear respiratory factor 1 (NRF1) and their target genes were repressed by RIP140 and induced by PGC-1α in a dose dependent manner in neonatal rat cardiomyocytes. We also observed that overexpression of RIP140 through adenovirus delivery can abrogate the PGC-1α-mediated induction of mitochondrial membrane potential elevation and mitochondrial biogenesis, and activate both autophagy and apoptosis pathways. We conclude that RIP140 and PGC-1α exert antagonistic role in regulating cardiac energy state and mitochondrial biogenesis.

Authors+Show Affiliations

Department of Pharmacology and Toxicology, School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22503866

Citation

Chen, Yanfang, et al. "Roles of Transcriptional Corepressor RIP140 and Coactivator PGC-1α in Energy State of Chronically Infarcted Rat Hearts and Mitochondrial Function of Cardiomyocytes." Molecular and Cellular Endocrinology, vol. 362, no. 1-2, 2012, pp. 11-8.
Chen Y, Wang Y, Chen J, et al. Roles of transcriptional corepressor RIP140 and coactivator PGC-1α in energy state of chronically infarcted rat hearts and mitochondrial function of cardiomyocytes. Mol Cell Endocrinol. 2012;362(1-2):11-8.
Chen, Y., Wang, Y., Chen, J., Chen, X., Cao, W., Chen, S., Xu, S., Huang, H., & Liu, P. (2012). Roles of transcriptional corepressor RIP140 and coactivator PGC-1α in energy state of chronically infarcted rat hearts and mitochondrial function of cardiomyocytes. Molecular and Cellular Endocrinology, 362(1-2), 11-8. https://doi.org/10.1016/j.mce.2012.03.023
Chen Y, et al. Roles of Transcriptional Corepressor RIP140 and Coactivator PGC-1α in Energy State of Chronically Infarcted Rat Hearts and Mitochondrial Function of Cardiomyocytes. Mol Cell Endocrinol. 2012 Oct 15;362(1-2):11-8. PubMed PMID: 22503866.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Roles of transcriptional corepressor RIP140 and coactivator PGC-1α in energy state of chronically infarcted rat hearts and mitochondrial function of cardiomyocytes. AU - Chen,Yanfang, AU - Wang,Yuhua, AU - Chen,Jianwen, AU - Chen,Xi, AU - Cao,Weiwei, AU - Chen,Shaorui, AU - Xu,Suowen, AU - Huang,Heqing, AU - Liu,Peiqing, Y1 - 2012/04/05/ PY - 2011/12/04/received PY - 2012/02/25/revised PY - 2012/03/30/accepted PY - 2012/4/17/entrez PY - 2012/4/17/pubmed PY - 2013/1/5/medline SP - 11 EP - 8 JF - Molecular and cellular endocrinology JO - Mol. Cell. Endocrinol. VL - 362 IS - 1-2 N2 - Transcriptional coactivator PPARγ coactivator-1α (PGC-1α) and corepressor receptor-interacting protein 140 (RIP140) are opposing-functional regulators in maintaining energy balance of most metabolic tissues and cells. However, the relative contributions of both factors to energy metabolism in cardiomyocytes remain largely unknown. Herein, we reported that the relative protein levels of RIP140/PGC-1α were up-regulated in the failing hearts after chronic myocardial infarction (MI), and correlated negatively with the energy state index phosphocreatine (PCr)/ATP ratios. Real-time PCR analysis revealed that mRNA expressions of estrogen related receptor α (ERRα), peroxisome proliferate activated receptor α and β (PPARα, PPARβ), nuclear respiratory factor 1 (NRF1) and their target genes were repressed by RIP140 and induced by PGC-1α in a dose dependent manner in neonatal rat cardiomyocytes. We also observed that overexpression of RIP140 through adenovirus delivery can abrogate the PGC-1α-mediated induction of mitochondrial membrane potential elevation and mitochondrial biogenesis, and activate both autophagy and apoptosis pathways. We conclude that RIP140 and PGC-1α exert antagonistic role in regulating cardiac energy state and mitochondrial biogenesis. SN - 1872-8057 UR - https://www.unboundmedicine.com/medline/citation/22503866/Roles_of_transcriptional_corepressor_RIP140_and_coactivator_PGC_1α_in_energy_state_of_chronically_infarcted_rat_hearts_and_mitochondrial_function_of_cardiomyocytes_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0303-7207(12)00217-1 DB - PRIME DP - Unbound Medicine ER -