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Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata.
J Ethnopharmacol. 2012 Jun 14; 141(3):1041-50.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

There is an increase in antimicrobial resistance and complexities arising from verotoxic related bacterial infections as well as rise in demand for application of natural antioxidants to combat oxidative damage by free radicals in many oxidative stress-mediated disease conditions such as cancer. Thus the potential of Curtisia dentata as antimicrobial, antioxidant and antiverotoxin against environmental isolates of Escherichia coli and Acinetobacter spp. as well as the presence of phytochemicals and some organic compounds, was determined.

MATERIALS AND METHODS

Phytochemical analysis was carried out using standard methods and antioxidant activity was determined using the DPPH radical scavenging activity. Effect of extracts on bacterial cell wall was also determined.

RESULTS

Extracts contained anthraquinones, alkaloids, essential oils, glycosides, phenols, steroids, saponins, tannins, quinones, anthocyanins, amines and carboxylic acids as phytochemicals. Extracts demonstrated high antimicrobial activity and low minimum inhibitory concentrations as well as inhibitory action against the expression of both Vtx1 and Vtx2 genes in Escherichia coli, Acinetobacter haemolyticus and Acinetobacter lwoffii. Ethanol root bark extracts consistently showed the highest DPPH radical scavenging activity (62.43%), total phenol content (TPH) (57.62 26 mg GAE/g) and reducing power (RP) (41.32%), followed by those of the stem bark and leaf extracts with the respective values of 54.68%, 37.77 mg GAE/g and 21.83%. The extracts induced the leakage of Na(+) and K(+) ions from both test bacteria.

CONCLUSION

Curtisia dentata is a very effective source of antioxidant and a possible alternative to sourcing antiverotoxic antibiotics with novel mechanism of action.

Authors+Show Affiliations

Faculty of Applied Sciences, Cape Peninsula University of Technology, P.O. Box 652, Cape Town 8000, South Africa. jameshamuel@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22504170

Citation

Doughari, James Hamuel, et al. "Antioxidant, Antimicrobial and Antiverotoxic Potentials of Extracts of Curtisia Dentata." Journal of Ethnopharmacology, vol. 141, no. 3, 2012, pp. 1041-50.
Doughari JH, Ndakidemi PA, Human IS, et al. Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata. J Ethnopharmacol. 2012;141(3):1041-50.
Doughari, J. H., Ndakidemi, P. A., Human, I. S., & Benade, S. (2012). Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata. Journal of Ethnopharmacology, 141(3), 1041-50. https://doi.org/10.1016/j.jep.2012.03.051
Doughari JH, et al. Antioxidant, Antimicrobial and Antiverotoxic Potentials of Extracts of Curtisia Dentata. J Ethnopharmacol. 2012 Jun 14;141(3):1041-50. PubMed PMID: 22504170.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antioxidant, antimicrobial and antiverotoxic potentials of extracts of Curtisia dentata. AU - Doughari,James Hamuel, AU - Ndakidemi,Patrick Alois, AU - Human,Izanne Susan, AU - Benade,Spinney, Y1 - 2012/04/06/ PY - 2012/01/31/received PY - 2012/03/10/revised PY - 2012/03/30/accepted PY - 2012/4/17/entrez PY - 2012/4/17/pubmed PY - 2012/9/29/medline SP - 1041 EP - 50 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 141 IS - 3 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: There is an increase in antimicrobial resistance and complexities arising from verotoxic related bacterial infections as well as rise in demand for application of natural antioxidants to combat oxidative damage by free radicals in many oxidative stress-mediated disease conditions such as cancer. Thus the potential of Curtisia dentata as antimicrobial, antioxidant and antiverotoxin against environmental isolates of Escherichia coli and Acinetobacter spp. as well as the presence of phytochemicals and some organic compounds, was determined. MATERIALS AND METHODS: Phytochemical analysis was carried out using standard methods and antioxidant activity was determined using the DPPH radical scavenging activity. Effect of extracts on bacterial cell wall was also determined. RESULTS: Extracts contained anthraquinones, alkaloids, essential oils, glycosides, phenols, steroids, saponins, tannins, quinones, anthocyanins, amines and carboxylic acids as phytochemicals. Extracts demonstrated high antimicrobial activity and low minimum inhibitory concentrations as well as inhibitory action against the expression of both Vtx1 and Vtx2 genes in Escherichia coli, Acinetobacter haemolyticus and Acinetobacter lwoffii. Ethanol root bark extracts consistently showed the highest DPPH radical scavenging activity (62.43%), total phenol content (TPH) (57.62 26 mg GAE/g) and reducing power (RP) (41.32%), followed by those of the stem bark and leaf extracts with the respective values of 54.68%, 37.77 mg GAE/g and 21.83%. The extracts induced the leakage of Na(+) and K(+) ions from both test bacteria. CONCLUSION: Curtisia dentata is a very effective source of antioxidant and a possible alternative to sourcing antiverotoxic antibiotics with novel mechanism of action. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22504170/Antioxidant_antimicrobial_and_antiverotoxic_potentials_of_extracts_of_Curtisia_dentata_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00213-9 DB - PRIME DP - Unbound Medicine ER -