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Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells.
J Agric Food Chem. 2012 May 09; 60(18):4628-33.JA

Abstract

Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with flavonoids for 24 h. Chrysin decreased the TER, indicating a decrease in TJ integrity. Daidzein, hesperetin, naringenin, and morin increased the TER, indicating increased TJ integrity. Luteolin and genistein increased or normalized the TER after a transient decrease. Immunoblot analysis revealed that these changes in TER were caused by modification of the cytoskeletal association and expression of TJ proteins, zonula occludens (ZO)-1, ZO-2, occludin, junctional adhesion molecule-1, and/or claudins. Our results suggest that various flavonoids participate in the regulation of intestinal TJ barrier integrity and that this regulation may partially contribute to the flavonoid-mediated biological effects on our health.

Authors+Show Affiliations

Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22506771

Citation

Noda, Sakino, et al. "Differential Effects of Flavonoids On Barrier Integrity in Human Intestinal Caco-2 Cells." Journal of Agricultural and Food Chemistry, vol. 60, no. 18, 2012, pp. 4628-33.
Noda S, Tanabe S, Suzuki T. Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells. J Agric Food Chem. 2012;60(18):4628-33.
Noda, S., Tanabe, S., & Suzuki, T. (2012). Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells. Journal of Agricultural and Food Chemistry, 60(18), 4628-33. https://doi.org/10.1021/jf300382h
Noda S, Tanabe S, Suzuki T. Differential Effects of Flavonoids On Barrier Integrity in Human Intestinal Caco-2 Cells. J Agric Food Chem. 2012 May 9;60(18):4628-33. PubMed PMID: 22506771.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Differential effects of flavonoids on barrier integrity in human intestinal Caco-2 cells. AU - Noda,Sakino, AU - Tanabe,Soichi, AU - Suzuki,Takuya, Y1 - 2012/04/24/ PY - 2012/4/18/entrez PY - 2012/4/18/pubmed PY - 2012/9/11/medline SP - 4628 EP - 33 JF - Journal of agricultural and food chemistry JO - J Agric Food Chem VL - 60 IS - 18 N2 - Flavonoids, present in fruits, vegetables, and teas, provide beneficial effects for our health. We investigated the effect of a number of flavonoids on tight junction (TJ) barrier integrity in human intestinal Caco-2 cells. Transepithelial electrical resistance (TER; a TJ integrity marker) across cell monolayers was measured in cells incubated with flavonoids for 24 h. Chrysin decreased the TER, indicating a decrease in TJ integrity. Daidzein, hesperetin, naringenin, and morin increased the TER, indicating increased TJ integrity. Luteolin and genistein increased or normalized the TER after a transient decrease. Immunoblot analysis revealed that these changes in TER were caused by modification of the cytoskeletal association and expression of TJ proteins, zonula occludens (ZO)-1, ZO-2, occludin, junctional adhesion molecule-1, and/or claudins. Our results suggest that various flavonoids participate in the regulation of intestinal TJ barrier integrity and that this regulation may partially contribute to the flavonoid-mediated biological effects on our health. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22506771/Differential_effects_of_flavonoids_on_barrier_integrity_in_human_intestinal_Caco_2_cells_ L2 - https://doi.org/10.1021/jf300382h DB - PRIME DP - Unbound Medicine ER -