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Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina.
Foodborne Pathog Dis. 2012 May; 9(5):457-64.FP

Abstract

Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx₂/stx(2c(vh-a)) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx₂ (20.8%) in human and stx(2c(vh-a)) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx₂/stx(2c(vh-a)) (12 humans and one bovine), PT4-AREXH01.0543-stx₂/stx(2c(vh-a)) (one human and four bovines), PT2-AREXH01.0076-stx₂/stx(2c(vh-a)) (one human and four bovines), PT49-AREXH01.0175-stx₂/stx(2c(vh-a)) (seven humans and one bovine), and PT49-AREXH01.0022-stx₂/stx(2c(vh-a)) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms.

Authors+Show Affiliations

Servicio Fisiopatogenia, Departamento Bacteriología, INEI-ANLIS "Dr. Carlos G. Malbrán," Buenos Aires, Argentina. bdastek@anlis.gov.arNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22506961

Citation

D'Astek, Beatriz A., et al. "Subtyping of Escherichia Coli O157:H7 Strains Isolated From Human Infections and Healthy Cattle in Argentina." Foodborne Pathogens and Disease, vol. 9, no. 5, 2012, pp. 457-64.
D'Astek BA, del Castillo LL, Miliwebsky E, et al. Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. Foodborne Pathog Dis. 2012;9(5):457-64.
D'Astek, B. A., del Castillo, L. L., Miliwebsky, E., Carbonari, C., Palladino, P. M., Deza, N., Chinen, I., Manfredi, E., Leotta, G. A., Masana, M. O., & Rivas, M. (2012). Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. Foodborne Pathogens and Disease, 9(5), 457-64. https://doi.org/10.1089/fpd.2011.1062
D'Astek BA, et al. Subtyping of Escherichia Coli O157:H7 Strains Isolated From Human Infections and Healthy Cattle in Argentina. Foodborne Pathog Dis. 2012;9(5):457-64. PubMed PMID: 22506961.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Subtyping of Escherichia coli O157:H7 strains isolated from human infections and healthy cattle in Argentina. AU - D'Astek,Beatriz A, AU - del Castillo,Lourdes L, AU - Miliwebsky,Elizabeth, AU - Carbonari,Claudia, AU - Palladino,Pablo M, AU - Deza,Natalia, AU - Chinen,Isabel, AU - Manfredi,Eduardo, AU - Leotta,Gerardo A, AU - Masana,Marcelo O, AU - Rivas,Marta, Y1 - 2012/04/16/ PY - 2012/4/18/entrez PY - 2012/4/18/pubmed PY - 2012/9/20/medline SP - 457 EP - 64 JF - Foodborne pathogens and disease JO - Foodborne Pathog Dis VL - 9 IS - 5 N2 - Shiga toxin-producing Escherichia coli (STEC) cause nonbloody (NBD) and bloody diarrhea (BD), and hemolytic uremic syndrome (HUS). Cattle have been described as their main reservoir. STEC O157:H7 is recognized as the predominant serotype in clinical infections, but much less is known about the dominant subtypes in humans and animals or their genetic relatedness. The aims of this study were to compare the STEC O157 subtypes found in sporadic human infections with those in the bovine reservoir using stx-genotyping, phage typing, and XbaI-pulsed-field gel electrophoresis (PFGE), and correlate the subtypes with the severity of clinical manifestations. The 280 STEC O157:H7 strains collected included in this study were isolated from HUS (n=122), BD (n=69), and NBD (n=30) cases, and healthy carriers (n=5), and from bovines (n=54) in the abattoirs. The stx-genotyping showed that stx₂/stx(2c(vh-a)) was predominant in human (76.1%) and in bovine strains (55.5%), whereas the second more important genotype was stx₂ (20.8%) in human and stx(2c(vh-a)) (16.7%) in cattle strains. In human strains, PT4 (37.6%), PT49 (24.3%), and PT2 (18.6%) were the most frequent PTs (80.5%). In bovine isolates, PT2 (26%), PT39 (16.7%), and PT4 and PT49 (11.1% each) were predominant. By XbaI-PFGE, all 280 strains yielded 148 patterns with 75% similarity, and 169 strains were grouped in 37 clusters. Identical PT-PFGE-stx profile combinations were detected in strains of both origins: PT4-AREXH01.0011-stx₂/stx(2c(vh-a)) (12 humans and one bovine), PT4-AREXH01.0543-stx₂/stx(2c(vh-a)) (one human and four bovines), PT2-AREXH01.0076-stx₂/stx(2c(vh-a)) (one human and four bovines), PT49-AREXH01.0175-stx₂/stx(2c(vh-a)) (seven humans and one bovine), and PT49-AREXH01.0022-stx₂/stx(2c(vh-a)) (seven humans and one bovine). No correlation was found among the stx-genotypes, the phage type, and the clinical symptoms. SN - 1556-7125 UR - https://www.unboundmedicine.com/medline/citation/22506961/Subtyping_of_Escherichia_coli_O157:H7_strains_isolated_from_human_infections_and_healthy_cattle_in_Argentina_ L2 - https://www.liebertpub.com/doi/10.1089/fpd.2011.1062?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -