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Effect of scaling and root planing on interleukin-1β, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients.
J Periodontal Res 2012; 47(6):681-8JP

Abstract

BACKGROUND AND OBJECTIVE

There are few data concerning the effect of scaling and root planing on the levels of immune and inflammatory mediators in gingival crevicular fluid from patients with chronic periodontitis. Therefore, in this study the influence of scaling and root planing was determined on amounts of interleukin (IL)-1β, IL-8 and MMP-8 in gingival crevicular fluid from patients with chronic periodontitis, in relation to clinical parameters.

MATERIAL AND METHODS

A total of 51 patients were enrolled in this study. The study population consisted of 30 patients with generalized advanced chronic periodontitis, while 21 periodontally healthy subjects were recruited for the control group. The clinical parameters included approximal plaque index, gingival index, pocket depth and clinical attachment loss. The amounts of IL-1β, IL-8 and MMP-8 in gingival crevicular fluid were measured by ELISA. Periodontal parameters as well as gingival crevicular fluid humoral factor amounts were evaluated in the control group and in chronic periodontitis patients at baseline and at 1 and 4 wk after scaling and root planing treatment.

RESULTS

At baseline, there were significant differences between control subjects and chronic periodontitis patients in terms of clinical attachment loss, pocket depth, gingival index (p < 0.001) and approximal plaque index (p < 0.01). The amounts of IL-1β, MMP-8 (p < 0.001) and IL-8 (p < 0.01) in gingival crevicular fluid were significantly lower in healthy subjects than in chronic periodontitis patients. Scaling and root planing led to improvement in all examined clinical parameters, apart from clinical attachment loss. Periodontal treatment also resulted in a significant decrease in the amounts of IL-1β, IL-8 and MMP-8 in comparison to baseline, especially 4 wk after scaling and root planing (p < 0.001); however, the amounts of these humoral factors were still higher than those in control group.

CONCLUSION

Our observations indicated that short-term nonsurgical therapy resulted in a significant improvement in periodontal indices and in a marked decrease of IL-1β, IL-8 and MMP-8 gingival crevicular fluid levels. Nevertheless, no significant correlations were found between clinical parameters and amounts of humoral factors after therapy.

Authors+Show Affiliations

Department of Experimental Immunology, Medical University of Łódź, Łódź, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22510045

Citation

Konopka, L, et al. "Effect of Scaling and Root Planing On Interleukin-1β, Interleukin-8 and MMP-8 Levels in Gingival Crevicular Fluid From Chronic Periodontitis Patients." Journal of Periodontal Research, vol. 47, no. 6, 2012, pp. 681-8.
Konopka L, Pietrzak A, Brzezińska-Błaszczyk E. Effect of scaling and root planing on interleukin-1β, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients. J Periodont Res. 2012;47(6):681-8.
Konopka, L., Pietrzak, A., & Brzezińska-Błaszczyk, E. (2012). Effect of scaling and root planing on interleukin-1β, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients. Journal of Periodontal Research, 47(6), pp. 681-8. doi:10.1111/j.1600-0765.2012.01480.x.
Konopka L, Pietrzak A, Brzezińska-Błaszczyk E. Effect of Scaling and Root Planing On Interleukin-1β, Interleukin-8 and MMP-8 Levels in Gingival Crevicular Fluid From Chronic Periodontitis Patients. J Periodont Res. 2012;47(6):681-8. PubMed PMID: 22510045.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of scaling and root planing on interleukin-1β, interleukin-8 and MMP-8 levels in gingival crevicular fluid from chronic periodontitis patients. AU - Konopka,L, AU - Pietrzak,A, AU - Brzezińska-Błaszczyk,E, Y1 - 2012/04/18/ PY - 2012/4/19/entrez PY - 2012/4/19/pubmed PY - 2013/5/4/medline SP - 681 EP - 8 JF - Journal of periodontal research JO - J. Periodont. Res. VL - 47 IS - 6 N2 - BACKGROUND AND OBJECTIVE: There are few data concerning the effect of scaling and root planing on the levels of immune and inflammatory mediators in gingival crevicular fluid from patients with chronic periodontitis. Therefore, in this study the influence of scaling and root planing was determined on amounts of interleukin (IL)-1β, IL-8 and MMP-8 in gingival crevicular fluid from patients with chronic periodontitis, in relation to clinical parameters. MATERIAL AND METHODS: A total of 51 patients were enrolled in this study. The study population consisted of 30 patients with generalized advanced chronic periodontitis, while 21 periodontally healthy subjects were recruited for the control group. The clinical parameters included approximal plaque index, gingival index, pocket depth and clinical attachment loss. The amounts of IL-1β, IL-8 and MMP-8 in gingival crevicular fluid were measured by ELISA. Periodontal parameters as well as gingival crevicular fluid humoral factor amounts were evaluated in the control group and in chronic periodontitis patients at baseline and at 1 and 4 wk after scaling and root planing treatment. RESULTS: At baseline, there were significant differences between control subjects and chronic periodontitis patients in terms of clinical attachment loss, pocket depth, gingival index (p < 0.001) and approximal plaque index (p < 0.01). The amounts of IL-1β, MMP-8 (p < 0.001) and IL-8 (p < 0.01) in gingival crevicular fluid were significantly lower in healthy subjects than in chronic periodontitis patients. Scaling and root planing led to improvement in all examined clinical parameters, apart from clinical attachment loss. Periodontal treatment also resulted in a significant decrease in the amounts of IL-1β, IL-8 and MMP-8 in comparison to baseline, especially 4 wk after scaling and root planing (p < 0.001); however, the amounts of these humoral factors were still higher than those in control group. CONCLUSION: Our observations indicated that short-term nonsurgical therapy resulted in a significant improvement in periodontal indices and in a marked decrease of IL-1β, IL-8 and MMP-8 gingival crevicular fluid levels. Nevertheless, no significant correlations were found between clinical parameters and amounts of humoral factors after therapy. SN - 1600-0765 UR - https://www.unboundmedicine.com/medline/citation/22510045/Effect_of_scaling_and_root_planing_on_interleukin_1β_interleukin_8_and_MMP_8_levels_in_gingival_crevicular_fluid_from_chronic_periodontitis_patients_ L2 - https://doi.org/10.1111/j.1600-0765.2012.01480.x DB - PRIME DP - Unbound Medicine ER -