Tags

Type your tag names separated by a space and hit enter

Diagnosis of soil-transmitted helminthiasis in an Amazonic community of Peru using multiple diagnostic techniques.
Trans R Soc Trop Med Hyg. 2012 Jun; 106(6):333-9.TR

Abstract

An observational descriptive study was conducted in a Shipibo-Conibo/Ese'Eja community of the rainforest in Peru to compare the Kato-Katz method and the spontaneous sedimentation in tube technique (SSTT) for the diagnosis of intestinal parasites as well as to report the prevalence of soil-transmitted helminth (STH) infections in this area. A total of 73 stool samples were collected and analysed by several parasitological techniques, including Kato-Katz, SSTT, modified Baermann technique (MBT), agar plate culture, Harada-Mori culture and the direct smear examination. Kato-Katz and SSTT had the same rate of detection for Ascaris lumbricoides (5%), Trichuris trichiura (5%), hookworm (14%) and Hymenolepis nana (26%). The detection rate for Strongyloides stercoralis larvae was 16% by SSTT and 0% by Kato-Katz, but 18% by agar plate culture and 16% by MBT. The SSTT also had the advantage of detecting multiple intestinal protozoa such as Blastocystis hominis (40%), Giardia intestinalis (29%) and Entamoeba histolytica/E. dispar (16%). The most common intestinal parasites found in this community were B. hominis, G. intestinalis, H. nana, S. stercoralis and hookworm. In conclusion, the SSTT is not inferior to Kato-Katz for the diagnosis of common STH infections but is largely superior for detecting intestinal protozoa and S. stercoralis larvae.

Authors+Show Affiliations

Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru. jorge.d.machicadorivero@uth.tmc.eduNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22515992

Citation

Machicado, Jorge D., et al. "Diagnosis of Soil-transmitted Helminthiasis in an Amazonic Community of Peru Using Multiple Diagnostic Techniques." Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 106, no. 6, 2012, pp. 333-9.
Machicado JD, Marcos LA, Tello R, et al. Diagnosis of soil-transmitted helminthiasis in an Amazonic community of Peru using multiple diagnostic techniques. Trans R Soc Trop Med Hyg. 2012;106(6):333-9.
Machicado, J. D., Marcos, L. A., Tello, R., Canales, M., Terashima, A., & Gotuzzo, E. (2012). Diagnosis of soil-transmitted helminthiasis in an Amazonic community of Peru using multiple diagnostic techniques. Transactions of the Royal Society of Tropical Medicine and Hygiene, 106(6), 333-9. https://doi.org/10.1016/j.trstmh.2012.03.004
Machicado JD, et al. Diagnosis of Soil-transmitted Helminthiasis in an Amazonic Community of Peru Using Multiple Diagnostic Techniques. Trans R Soc Trop Med Hyg. 2012;106(6):333-9. PubMed PMID: 22515992.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Diagnosis of soil-transmitted helminthiasis in an Amazonic community of Peru using multiple diagnostic techniques. AU - Machicado,Jorge D, AU - Marcos,Luis A, AU - Tello,Raul, AU - Canales,Marco, AU - Terashima,Angelica, AU - Gotuzzo,Eduardo, Y1 - 2012/04/17/ PY - 2011/03/23/received PY - 2012/03/14/revised PY - 2012/03/14/accepted PY - 2012/4/21/entrez PY - 2012/4/21/pubmed PY - 2012/9/27/medline SP - 333 EP - 9 JF - Transactions of the Royal Society of Tropical Medicine and Hygiene JO - Trans R Soc Trop Med Hyg VL - 106 IS - 6 N2 - An observational descriptive study was conducted in a Shipibo-Conibo/Ese'Eja community of the rainforest in Peru to compare the Kato-Katz method and the spontaneous sedimentation in tube technique (SSTT) for the diagnosis of intestinal parasites as well as to report the prevalence of soil-transmitted helminth (STH) infections in this area. A total of 73 stool samples were collected and analysed by several parasitological techniques, including Kato-Katz, SSTT, modified Baermann technique (MBT), agar plate culture, Harada-Mori culture and the direct smear examination. Kato-Katz and SSTT had the same rate of detection for Ascaris lumbricoides (5%), Trichuris trichiura (5%), hookworm (14%) and Hymenolepis nana (26%). The detection rate for Strongyloides stercoralis larvae was 16% by SSTT and 0% by Kato-Katz, but 18% by agar plate culture and 16% by MBT. The SSTT also had the advantage of detecting multiple intestinal protozoa such as Blastocystis hominis (40%), Giardia intestinalis (29%) and Entamoeba histolytica/E. dispar (16%). The most common intestinal parasites found in this community were B. hominis, G. intestinalis, H. nana, S. stercoralis and hookworm. In conclusion, the SSTT is not inferior to Kato-Katz for the diagnosis of common STH infections but is largely superior for detecting intestinal protozoa and S. stercoralis larvae. SN - 1878-3503 UR - https://www.unboundmedicine.com/medline/citation/22515992/Diagnosis_of_soil_transmitted_helminthiasis_in_an_Amazonic_community_of_Peru_using_multiple_diagnostic_techniques_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0035-9203(12)00050-8 DB - PRIME DP - Unbound Medicine ER -