Tags

Type your tag names separated by a space and hit enter

High-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes.
J Chromatogr A. 2012 Jun 01; 1240:96-103.JC

Abstract

Evaporative light scattering detector (ELSD) is considered as a universal detector able to detect almost any compound. This study successfully established a HPLC-ELSD method for the simultaneous determination of phenolic acids, flavonoids and anthocyanins in muscadine grape. Twenty-four phenolics, including 5 anthocyanins, were identified by HPLC-UV-MS, ten of them were selected to calibrate the ELSD. Results from the ELSD quantification suggested that gallic acid, proanthocyanidins, and ellagic acid were the main phenolics in muscadines. In addition, antioxidant tests suggested that the extracts of muscadine grapes possessed strong antioxidant activities. All extracts had a high total phenolic content (TPC). High total anthocyanins contents (TAC) were found in Noble muscadine, and high proanthocyanidins content (TPA) in the seed portion.

Authors+Show Affiliations

Department of Food, Nutrition and Packaging Sciences, Clemson University, Clemson, SC 29634, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22520637

Citation

You, Qi, et al. "High-performance Liquid Chromatography-mass Spectrometry and Evaporative Light-scattering Detector to Compare Phenolic Profiles of Muscadine Grapes." Journal of Chromatography. A, vol. 1240, 2012, pp. 96-103.
You Q, Chen F, Sharp JL, et al. High-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes. J Chromatogr A. 2012;1240:96-103.
You, Q., Chen, F., Sharp, J. L., Wang, X., You, Y., & Zhang, C. (2012). High-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes. Journal of Chromatography. A, 1240, 96-103. https://doi.org/10.1016/j.chroma.2012.03.086
You Q, et al. High-performance Liquid Chromatography-mass Spectrometry and Evaporative Light-scattering Detector to Compare Phenolic Profiles of Muscadine Grapes. J Chromatogr A. 2012 Jun 1;1240:96-103. PubMed PMID: 22520637.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - High-performance liquid chromatography-mass spectrometry and evaporative light-scattering detector to compare phenolic profiles of muscadine grapes. AU - You,Qi, AU - Chen,Feng, AU - Sharp,Julia L, AU - Wang,Xi, AU - You,Yuru, AU - Zhang,Chuanji, Y1 - 2012/04/04/ PY - 2012/01/27/received PY - 2012/03/24/revised PY - 2012/03/26/accepted PY - 2012/4/24/entrez PY - 2012/4/24/pubmed PY - 2012/6/27/medline SP - 96 EP - 103 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1240 N2 - Evaporative light scattering detector (ELSD) is considered as a universal detector able to detect almost any compound. This study successfully established a HPLC-ELSD method for the simultaneous determination of phenolic acids, flavonoids and anthocyanins in muscadine grape. Twenty-four phenolics, including 5 anthocyanins, were identified by HPLC-UV-MS, ten of them were selected to calibrate the ELSD. Results from the ELSD quantification suggested that gallic acid, proanthocyanidins, and ellagic acid were the main phenolics in muscadines. In addition, antioxidant tests suggested that the extracts of muscadine grapes possessed strong antioxidant activities. All extracts had a high total phenolic content (TPC). High total anthocyanins contents (TAC) were found in Noble muscadine, and high proanthocyanidins content (TPA) in the seed portion. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/22520637/High_performance_liquid_chromatography_mass_spectrometry_and_evaporative_light_scattering_detector_to_compare_phenolic_profiles_of_muscadine_grapes_ DB - PRIME DP - Unbound Medicine ER -