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Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation.
New Phytol. 2012 Jul; 195(1):113-23.NP

Abstract

• In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem.

Authors+Show Affiliations

Robert W. Holley Center for Agriculture and Health, USDA-ARS, Cornell University, Ithaca, NY 14853, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22524643

Citation

Milner, Matthew J., et al. "Characterization of the High Affinity Zn Transporter From Noccaea Caerulescens, NcZNT1, and Dissection of Its Promoter for Its Role in Zn Uptake and Hyperaccumulation." The New Phytologist, vol. 195, no. 1, 2012, pp. 113-23.
Milner MJ, Craft E, Yamaji N, et al. Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation. New Phytol. 2012;195(1):113-23.
Milner, M. J., Craft, E., Yamaji, N., Koyama, E., Ma, J. F., & Kochian, L. V. (2012). Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation. The New Phytologist, 195(1), 113-23. https://doi.org/10.1111/j.1469-8137.2012.04144.x
Milner MJ, et al. Characterization of the High Affinity Zn Transporter From Noccaea Caerulescens, NcZNT1, and Dissection of Its Promoter for Its Role in Zn Uptake and Hyperaccumulation. New Phytol. 2012;195(1):113-23. PubMed PMID: 22524643.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of the high affinity Zn transporter from Noccaea caerulescens, NcZNT1, and dissection of its promoter for its role in Zn uptake and hyperaccumulation. AU - Milner,Matthew J, AU - Craft,Eric, AU - Yamaji,Naoki, AU - Koyama,Emi, AU - Ma,Jian Feng, AU - Kochian,Leon V, Y1 - 2012/04/23/ PY - 2012/4/25/entrez PY - 2012/4/25/pubmed PY - 2012/10/10/medline SP - 113 EP - 23 JF - The New phytologist JO - New Phytol VL - 195 IS - 1 N2 - • In this paper, we conducted a detailed analysis of the ZIP family transporter, NcZNT1, in the zinc (Zn)/cadmium (Cd) hyperaccumulating plant species, Noccaea caerulescens, formerly known as Thlaspi caerulescens. NcZNT1 was previously suggested to be the primary root Zn/Cd uptake transporter. Both a characterization of NcZNT1 transport function in planta and in heterologous systems, and an analysis of NcZNT1 gene expression and NcZNT1 protein localization were carried out. • We show that NcZNT1 is not only expressed in the root epidermis, but also is highly expressed in the root and shoot vasculature, suggesting a role in long-distance metal transport. Also, NcZNT1 was found to be a plasma membrane transporter that mediates Zn but not Cd, iron (Fe), manganese (Mn) or copper (Cu) uptake into plant cells. • Two novel regions of the NcZNT1 promoter were identified which may be involved in both the hyperexpression of NcZNT1 and its ability to be regulated by plant Zn status. • In conclusion, we demonstrate here that NcZNT1 plays a role in Zn and not Cd uptake from the soil, and based on its strong expression in the root and shoot vasculature, could be involved in long-distance transport of Zn from the root to the shoot via the xylem. SN - 1469-8137 UR - https://www.unboundmedicine.com/medline/citation/22524643/Characterization_of_the_high_affinity_Zn_transporter_from_Noccaea_caerulescens_NcZNT1_and_dissection_of_its_promoter_for_its_role_in_Zn_uptake_and_hyperaccumulation_ L2 - https://doi.org/10.1111/j.1469-8137.2012.04144.x DB - PRIME DP - Unbound Medicine ER -