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Eryngium foetidum suppresses inflammatory mediators produced by macrophages.
Asian Pac J Cancer Prev 2012; 13(2):653-64AP

Abstract

OBJECTIVE

This study assessed anti-inflammatory and antioxidant activities of E. foetidum leaf extract on LPS-activated murine macrophages.

METHODS

RAW264.7 cells were pretreated with or without E. foetidum extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-α and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and IκB by Western blotting.

RESULTS

Prior treatment with E. foetidum leaf extract inhibited elevation of IL-6, TNF-α, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as IκB. E. foetidum ethanol extract was shown to contain lutein, β-carotenes, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties.

CONCLUSIONS

E. foetidum leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, E. foetidum has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation.

Authors+Show Affiliations

Institute of Nutrition, Mahidol University, Nakhon Pathom, Thailand.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22524841

Citation

Mekhora, Chusana, et al. "Eryngium Foetidum Suppresses Inflammatory Mediators Produced By Macrophages." Asian Pacific Journal of Cancer Prevention : APJCP, vol. 13, no. 2, 2012, pp. 653-64.
Mekhora C, Muangnoi C, Chingsuwanrote P, et al. Eryngium foetidum suppresses inflammatory mediators produced by macrophages. Asian Pac J Cancer Prev. 2012;13(2):653-64.
Mekhora, C., Muangnoi, C., Chingsuwanrote, P., Dawilai, S., Svasti, S., Chasri, K., & Tuntipopipat, S. (2012). Eryngium foetidum suppresses inflammatory mediators produced by macrophages. Asian Pacific Journal of Cancer Prevention : APJCP, 13(2), pp. 653-64.
Mekhora C, et al. Eryngium Foetidum Suppresses Inflammatory Mediators Produced By Macrophages. Asian Pac J Cancer Prev. 2012;13(2):653-64. PubMed PMID: 22524841.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Eryngium foetidum suppresses inflammatory mediators produced by macrophages. AU - Mekhora,Chusana, AU - Muangnoi,Channarong, AU - Chingsuwanrote,Pimjai, AU - Dawilai,Suwitcha, AU - Svasti,Saovaros, AU - Chasri,Kaimuk, AU - Tuntipopipat,Siriporn, PY - 2012/4/25/entrez PY - 2012/4/25/pubmed PY - 2012/9/21/medline SP - 653 EP - 64 JF - Asian Pacific journal of cancer prevention : APJCP JO - Asian Pac. J. Cancer Prev. VL - 13 IS - 2 N2 - OBJECTIVE: This study assessed anti-inflammatory and antioxidant activities of E. foetidum leaf extract on LPS-activated murine macrophages. METHODS: RAW264.7 cells were pretreated with or without E. foetidum extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-α and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and IκB by Western blotting. RESULTS: Prior treatment with E. foetidum leaf extract inhibited elevation of IL-6, TNF-α, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as IκB. E. foetidum ethanol extract was shown to contain lutein, β-carotenes, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties. CONCLUSIONS: E. foetidum leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, E. foetidum has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation. SN - 2476-762X UR - https://www.unboundmedicine.com/medline/citation/22524841/Eryngium_foetidum_suppresses_inflammatory_mediators_produced_by_macrophages_ L2 - http://journal.waocp.org/?sid=Entrez:PubMed&id=pmid:22524841&key=2012.13.2.653 DB - PRIME DP - Unbound Medicine ER -