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Prenatal and neonatal flutamide administration increases proliferation and reduces apoptosis in large antral follicles of adult pigs.
Anim Reprod Sci. 2012 May; 132(1-2):58-65.AR

Abstract

Ovarian follicular atresia is regulated by androgens directly via androgen receptors and indirectly after conversion to estrogens. The balance between proliferation and cell apoptosis is crucial for the physiological functioning of the follicles. The disorder between these processes leads to reproductive failure, such as cyst formation. Recent research suggests maternally or neonatally mediated effects of antiandrogen flutamide on reproductive functions during adulthood. Therefore, the current study was performed to determine whether late gestational or neonatal exposure to flutamide influences proliferation and apoptosis rates in large antral follicles of adult porcine ovary. These periods are critical in ovarian biology and may determine female fertility in adulthood. Flutamide was injected into pregnant gilts between days 80 and 88 of gestation, and into female piglets between days 2 and 10 postnatally. The ovaries were collected from treated and control adult pigs, and healthy large antral follicles were excised. In large antral follicles, granulosa and theca cells revealed elevated (p<0.001) proliferation index measured by immunolocalization of Ki-67 following maternal and neonatal flutamide exposure. The percentage of apoptotic granulosa cells detected using TUNEL assay significantly decreased (p<0.01) after flutamide administration, that paralleled with down-regulation (p<0.01) of caspase-3 protein expression. Moreover, plasma testosterone decreased (p<0.001) in flutamide-treated animals, whereas 17β-estradiol was elevated following flutamide exposure. The present research indicates increased proliferation and diminished apoptosis rates within large antral follicles of adult pigs following prenatal and neonatal flutamide administration, which might influence the normal development of the follicles and pigs fertility as a consequence.

Authors+Show Affiliations

Department of Endocrinology, Institute of Zoology, Jagiellonian University, Krakow, Poland. malgorzata.durlej@uj.edu.plNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22525094

Citation

Durlej, Malgorzata, et al. "Prenatal and Neonatal Flutamide Administration Increases Proliferation and Reduces Apoptosis in Large Antral Follicles of Adult Pigs." Animal Reproduction Science, vol. 132, no. 1-2, 2012, pp. 58-65.
Durlej M, Knapczyk-Stwora K, Slomczynska M. Prenatal and neonatal flutamide administration increases proliferation and reduces apoptosis in large antral follicles of adult pigs. Anim Reprod Sci. 2012;132(1-2):58-65.
Durlej, M., Knapczyk-Stwora, K., & Slomczynska, M. (2012). Prenatal and neonatal flutamide administration increases proliferation and reduces apoptosis in large antral follicles of adult pigs. Animal Reproduction Science, 132(1-2), 58-65. https://doi.org/10.1016/j.anireprosci.2012.04.001
Durlej M, Knapczyk-Stwora K, Slomczynska M. Prenatal and Neonatal Flutamide Administration Increases Proliferation and Reduces Apoptosis in Large Antral Follicles of Adult Pigs. Anim Reprod Sci. 2012;132(1-2):58-65. PubMed PMID: 22525094.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Prenatal and neonatal flutamide administration increases proliferation and reduces apoptosis in large antral follicles of adult pigs. AU - Durlej,Malgorzata, AU - Knapczyk-Stwora,Katarzyna, AU - Slomczynska,Maria, Y1 - 2012/04/07/ PY - 2012/01/20/received PY - 2012/03/26/revised PY - 2012/04/01/accepted PY - 2012/4/25/entrez PY - 2012/4/25/pubmed PY - 2012/10/27/medline SP - 58 EP - 65 JF - Animal reproduction science JO - Anim Reprod Sci VL - 132 IS - 1-2 N2 - Ovarian follicular atresia is regulated by androgens directly via androgen receptors and indirectly after conversion to estrogens. The balance between proliferation and cell apoptosis is crucial for the physiological functioning of the follicles. The disorder between these processes leads to reproductive failure, such as cyst formation. Recent research suggests maternally or neonatally mediated effects of antiandrogen flutamide on reproductive functions during adulthood. Therefore, the current study was performed to determine whether late gestational or neonatal exposure to flutamide influences proliferation and apoptosis rates in large antral follicles of adult porcine ovary. These periods are critical in ovarian biology and may determine female fertility in adulthood. Flutamide was injected into pregnant gilts between days 80 and 88 of gestation, and into female piglets between days 2 and 10 postnatally. The ovaries were collected from treated and control adult pigs, and healthy large antral follicles were excised. In large antral follicles, granulosa and theca cells revealed elevated (p<0.001) proliferation index measured by immunolocalization of Ki-67 following maternal and neonatal flutamide exposure. The percentage of apoptotic granulosa cells detected using TUNEL assay significantly decreased (p<0.01) after flutamide administration, that paralleled with down-regulation (p<0.01) of caspase-3 protein expression. Moreover, plasma testosterone decreased (p<0.001) in flutamide-treated animals, whereas 17β-estradiol was elevated following flutamide exposure. The present research indicates increased proliferation and diminished apoptosis rates within large antral follicles of adult pigs following prenatal and neonatal flutamide administration, which might influence the normal development of the follicles and pigs fertility as a consequence. SN - 1873-2232 UR - https://www.unboundmedicine.com/medline/citation/22525094/Prenatal_and_neonatal_flutamide_administration_increases_proliferation_and_reduces_apoptosis_in_large_antral_follicles_of_adult_pigs_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-4320(12)00108-X DB - PRIME DP - Unbound Medicine ER -