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Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals.
Int J Antimicrob Agents. 2012 Jun; 39(6):529-33.IJ

Abstract

The global spread of New Delhi metallo-β-lactamase (NDM) is of significant public health concern. This study sought to determine whether bla(NDM) was present in Enterobacteriaceae isolates displaying resistance to carbapenems that were submitted to the National Antibiotic Reference Laboratory, Institute of Environmental Science and Research (Porirua, New Zealand) during 2009 and 2010. Isolates were tested for the presence of β-lactamase genes and 16S rRNA methylase genes by polymerase chain reaction (PCR) and sequencing. Plasmid transfer studies were undertaken on isolates found to be harbouring bla(NDM). Molecular typing was performed by multilocus sequence typing (MLST). The bla(NDM-1) gene was identified in four Enterobacteriaceae isolates (two Escherichia coli, one Klebsiella pneumoniae and one Proteus mirabilis) from four patients in New Zealand hospitals in 2009 and 2010. In addition, the bla(NDM-6) gene, which differed from bla(NDM-1) by a point mutation at position 698 (C→T), was also identified in an E. coli isolate from the same patient who harboured the bla(NDM-1)-positive P. mirabilis. All four patients had recently been hospitalised or received health care in India. Four of the isolates also produced a CTX-M-15 extended-spectrum β-lactamase and/or plasmid-mediated AmpC β-lactamase, and all five isolates harboured the plasmid-mediated 16S rRNA methylase rmtC gene. The E. coli types were diverse by MLST, and the K. pneumoniae isolate belonged to the internationally disseminated sequence type 11 (ST11) clone. These findings further illustrate the diversity of phenotypic and genotypic features found in association with bla(NDM), in addition to documenting the international spread of this resistance mechanism, notably into a country with historically low rates of antimicrobial resistance.

Authors+Show Affiliations

Department of Clinical Microbiology, Auckland District Health Board, Park Road, Grafton, Auckland, New Zealand. dwilliamson@adhb.govt.nzNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22526013

Citation

Williamson, Deborah A., et al. "Identification and Molecular Characterisation of New Delhi Metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae From New Zealand Hospitals." International Journal of Antimicrobial Agents, vol. 39, no. 6, 2012, pp. 529-33.
Williamson DA, Sidjabat HE, Freeman JT, et al. Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. Int J Antimicrob Agents. 2012;39(6):529-33.
Williamson, D. A., Sidjabat, H. E., Freeman, J. T., Roberts, S. A., Silvey, A., Woodhouse, R., Mowat, E., Dyet, K., Paterson, D. L., Blackmore, T., Burns, A., & Heffernan, H. (2012). Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. International Journal of Antimicrobial Agents, 39(6), 529-33. https://doi.org/10.1016/j.ijantimicag.2012.02.017
Williamson DA, et al. Identification and Molecular Characterisation of New Delhi Metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae From New Zealand Hospitals. Int J Antimicrob Agents. 2012;39(6):529-33. PubMed PMID: 22526013.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and molecular characterisation of New Delhi metallo-β-lactamase-1 (NDM-1)- and NDM-6-producing Enterobacteriaceae from New Zealand hospitals. AU - Williamson,Deborah A, AU - Sidjabat,Hanna E, AU - Freeman,Joshua T, AU - Roberts,Sally A, AU - Silvey,Anna, AU - Woodhouse,Rosemary, AU - Mowat,Eilidh, AU - Dyet,Kristin, AU - Paterson,David L, AU - Blackmore,Timothy, AU - Burns,Andrew, AU - Heffernan,Helen, Y1 - 2012/04/21/ PY - 2011/12/20/received PY - 2012/02/29/revised PY - 2012/02/29/accepted PY - 2012/4/25/entrez PY - 2012/4/25/pubmed PY - 2012/9/6/medline SP - 529 EP - 33 JF - International journal of antimicrobial agents JO - Int. J. Antimicrob. Agents VL - 39 IS - 6 N2 - The global spread of New Delhi metallo-β-lactamase (NDM) is of significant public health concern. This study sought to determine whether bla(NDM) was present in Enterobacteriaceae isolates displaying resistance to carbapenems that were submitted to the National Antibiotic Reference Laboratory, Institute of Environmental Science and Research (Porirua, New Zealand) during 2009 and 2010. Isolates were tested for the presence of β-lactamase genes and 16S rRNA methylase genes by polymerase chain reaction (PCR) and sequencing. Plasmid transfer studies were undertaken on isolates found to be harbouring bla(NDM). Molecular typing was performed by multilocus sequence typing (MLST). The bla(NDM-1) gene was identified in four Enterobacteriaceae isolates (two Escherichia coli, one Klebsiella pneumoniae and one Proteus mirabilis) from four patients in New Zealand hospitals in 2009 and 2010. In addition, the bla(NDM-6) gene, which differed from bla(NDM-1) by a point mutation at position 698 (C→T), was also identified in an E. coli isolate from the same patient who harboured the bla(NDM-1)-positive P. mirabilis. All four patients had recently been hospitalised or received health care in India. Four of the isolates also produced a CTX-M-15 extended-spectrum β-lactamase and/or plasmid-mediated AmpC β-lactamase, and all five isolates harboured the plasmid-mediated 16S rRNA methylase rmtC gene. The E. coli types were diverse by MLST, and the K. pneumoniae isolate belonged to the internationally disseminated sequence type 11 (ST11) clone. These findings further illustrate the diversity of phenotypic and genotypic features found in association with bla(NDM), in addition to documenting the international spread of this resistance mechanism, notably into a country with historically low rates of antimicrobial resistance. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/22526013/Identification_and_molecular_characterisation_of_New_Delhi_metallo_β_lactamase_1__NDM_1___and_NDM_6_producing_Enterobacteriaceae_from_New_Zealand_hospitals_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(12)00106-9 DB - PRIME DP - Unbound Medicine ER -