Tags

Type your tag names separated by a space and hit enter

Gene delivery of antioxidant enzymes inhibits human immunodeficiency virus type 1 gp120-induced expression of caspases.
Neuroscience. 2012 Jul 12; 214:68-77.N

Abstract

Caspases are implicated in neuronal death in neurodegenerative and other central nervous system (CNS) diseases. In a rat model of human immunodeficiency virus type 1 (HIV-1) associated neurocognitive disorders (HAND), we previously characterized HIV-1 envelope gp120-induced neuronal apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. In this model, neuronal apoptosis occurred probably via gp120-induced reactive oxygen species (ROS). Antioxidant gene delivery blunted gp120-related apoptosis. Here, we studied the effect of gp120 on different caspases (3, 6, 8, 9) expression. Caspases production increased in the rat caudate-putamen (CP) 6h after gp120 injection into the same structure. The expression of caspases peaked by 24h. Caspases colocalized mainly with neurons. Prior gene delivery of the antioxidant enzymes Cu/Zn superoxide dismutase (SOD1) or glutathione peroxidase (GPx1) into the CP before injecting gp120 there reduced levels of gp120-induced caspases, recapitulating the effect of antioxidant enzymes on gp120-induced apoptosis observed by TUNEL. Thus, HIV-1 gp120 increased caspases expression in the CP. Prior antioxidant enzyme treatment mitigated production of these caspases, probably by reducing ROS levels.

Authors+Show Affiliations

Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, PA 19107, United States. jplouboutin@hotmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

22531373

Citation

Louboutin, J-P, et al. "Gene Delivery of Antioxidant Enzymes Inhibits Human Immunodeficiency Virus Type 1 Gp120-induced Expression of Caspases." Neuroscience, vol. 214, 2012, pp. 68-77.
Louboutin JP, Agrawal L, Reyes BA, et al. Gene delivery of antioxidant enzymes inhibits human immunodeficiency virus type 1 gp120-induced expression of caspases. Neuroscience. 2012;214:68-77.
Louboutin, J. P., Agrawal, L., Reyes, B. A., van Bockstaele, E. J., & Strayer, D. S. (2012). Gene delivery of antioxidant enzymes inhibits human immunodeficiency virus type 1 gp120-induced expression of caspases. Neuroscience, 214, 68-77. https://doi.org/10.1016/j.neuroscience.2012.03.061
Louboutin JP, et al. Gene Delivery of Antioxidant Enzymes Inhibits Human Immunodeficiency Virus Type 1 Gp120-induced Expression of Caspases. Neuroscience. 2012 Jul 12;214:68-77. PubMed PMID: 22531373.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Gene delivery of antioxidant enzymes inhibits human immunodeficiency virus type 1 gp120-induced expression of caspases. AU - Louboutin,J-P, AU - Agrawal,L, AU - Reyes,B A S, AU - van Bockstaele,E J, AU - Strayer,D S, Y1 - 2012/04/21/ PY - 2011/12/21/received PY - 2012/03/14/revised PY - 2012/03/16/accepted PY - 2012/4/26/entrez PY - 2012/4/26/pubmed PY - 2013/4/30/medline SP - 68 EP - 77 JF - Neuroscience JO - Neuroscience VL - 214 N2 - Caspases are implicated in neuronal death in neurodegenerative and other central nervous system (CNS) diseases. In a rat model of human immunodeficiency virus type 1 (HIV-1) associated neurocognitive disorders (HAND), we previously characterized HIV-1 envelope gp120-induced neuronal apoptosis by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. In this model, neuronal apoptosis occurred probably via gp120-induced reactive oxygen species (ROS). Antioxidant gene delivery blunted gp120-related apoptosis. Here, we studied the effect of gp120 on different caspases (3, 6, 8, 9) expression. Caspases production increased in the rat caudate-putamen (CP) 6h after gp120 injection into the same structure. The expression of caspases peaked by 24h. Caspases colocalized mainly with neurons. Prior gene delivery of the antioxidant enzymes Cu/Zn superoxide dismutase (SOD1) or glutathione peroxidase (GPx1) into the CP before injecting gp120 there reduced levels of gp120-induced caspases, recapitulating the effect of antioxidant enzymes on gp120-induced apoptosis observed by TUNEL. Thus, HIV-1 gp120 increased caspases expression in the CP. Prior antioxidant enzyme treatment mitigated production of these caspases, probably by reducing ROS levels. SN - 1873-7544 UR - https://www.unboundmedicine.com/medline/citation/22531373/Gene_delivery_of_antioxidant_enzymes_inhibits_human_immunodeficiency_virus_type_1_gp120_induced_expression_of_caspases_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0306-4522(12)00385-5 DB - PRIME DP - Unbound Medicine ER -