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Oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients.
Support Care Cancer. 2012 Dec; 20(12):3231-40.SC

Abstract

BACKGROUND

Oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (HSCT). Through HSCT, the homeostasis in the oral cavity is disrupted. The contribution of the oral microflora to mucositis remains to be clarified. The aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in HSCT recipients.

METHODS

This prospective observational study included 49 adult HSCT recipients. Twice weekly, oral ulcerations were scored, and oral rinsing samples were obtained. Samples were evaluated for the total bacterial load; the Gram-negative bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola; and the yeasts: Candida albicans, Candida glabrata, Candida kefyr, Candida krusei, Candida parapsilosis, and Candida tropicalis using real-time polymerase chain reaction with specific primers and probes. Explanatory variables for oral ulcerations were calculated using the multilevel generalized estimated equations (GEE) technique.

RESULTS

None of the samples was positive for A. actinomycetemcomitans, while F. nucleatum was found most often (66 % of samples). C. albicans was the most isolated yeast (88 % of samples), whereas C. parapsilosis was found in only 8 % of the samples. Multivariate GEE analyses identified P. gingivalis, P. micra, T. denticola, F. nucleatum, C. glabrata, and C. kefyr as significant explanatory variables of oral ulcerations.

CONCLUSIONS

Our data indicate that P. gingivalis in particular, but also P. micra, T. denticola, F. nucleatum, C. glabrata, and C. kefyr may play a role in ulcerative oral mucositis in patients undergoing HSCT.

Authors+Show Affiliations

Department of Preventive Dentistry, Academic Center for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Gustav Mahlerlaan 3004, 1081 LA Amsterdam, The Netherlands. a.lahey@acta.nl.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22531876

Citation

Laheij, Alexa M G A., et al. "Oral Bacteria and Yeasts in Relationship to Oral Ulcerations in Hematopoietic Stem Cell Transplant Recipients." Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer, vol. 20, no. 12, 2012, pp. 3231-40.
Laheij AM, de Soet JJ, von dem Borne PA, et al. Oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. Support Care Cancer. 2012;20(12):3231-40.
Laheij, A. M., de Soet, J. J., von dem Borne, P. A., Kuijper, E. J., Kraneveld, E. A., van Loveren, C., & Raber-Durlacher, J. E. (2012). Oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. Supportive Care in Cancer : Official Journal of the Multinational Association of Supportive Care in Cancer, 20(12), 3231-40. https://doi.org/10.1007/s00520-012-1463-2
Laheij AM, et al. Oral Bacteria and Yeasts in Relationship to Oral Ulcerations in Hematopoietic Stem Cell Transplant Recipients. Support Care Cancer. 2012;20(12):3231-40. PubMed PMID: 22531876.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Oral bacteria and yeasts in relationship to oral ulcerations in hematopoietic stem cell transplant recipients. AU - Laheij,Alexa M G A, AU - de Soet,Johannes J, AU - von dem Borne,Peter A, AU - Kuijper,Ed J, AU - Kraneveld,Eefje A, AU - van Loveren,Cor, AU - Raber-Durlacher,Judith E, Y1 - 2012/04/26/ PY - 2011/08/08/received PY - 2012/04/02/accepted PY - 2012/4/26/entrez PY - 2012/4/26/pubmed PY - 2013/5/15/medline SP - 3231 EP - 40 JF - Supportive care in cancer : official journal of the Multinational Association of Supportive Care in Cancer JO - Support Care Cancer VL - 20 IS - 12 N2 - BACKGROUND: Oral mucositis is a serious and debilitating side effect of conditioning regimens for hematopoietic stem cell transplant (HSCT). Through HSCT, the homeostasis in the oral cavity is disrupted. The contribution of the oral microflora to mucositis remains to be clarified. The aim of our study was to investigate the relationship between yeasts, bacteria associated with periodontitis, and oral ulcerations in HSCT recipients. METHODS: This prospective observational study included 49 adult HSCT recipients. Twice weekly, oral ulcerations were scored, and oral rinsing samples were obtained. Samples were evaluated for the total bacterial load; the Gram-negative bacteria: Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Prevotella intermedia, Parvimonas micra, Fusobacterium nucleatum, Tannerella forsythia, and Treponema denticola; and the yeasts: Candida albicans, Candida glabrata, Candida kefyr, Candida krusei, Candida parapsilosis, and Candida tropicalis using real-time polymerase chain reaction with specific primers and probes. Explanatory variables for oral ulcerations were calculated using the multilevel generalized estimated equations (GEE) technique. RESULTS: None of the samples was positive for A. actinomycetemcomitans, while F. nucleatum was found most often (66 % of samples). C. albicans was the most isolated yeast (88 % of samples), whereas C. parapsilosis was found in only 8 % of the samples. Multivariate GEE analyses identified P. gingivalis, P. micra, T. denticola, F. nucleatum, C. glabrata, and C. kefyr as significant explanatory variables of oral ulcerations. CONCLUSIONS: Our data indicate that P. gingivalis in particular, but also P. micra, T. denticola, F. nucleatum, C. glabrata, and C. kefyr may play a role in ulcerative oral mucositis in patients undergoing HSCT. SN - 1433-7339 UR - https://www.unboundmedicine.com/medline/citation/22531876/Oral_bacteria_and_yeasts_in_relationship_to_oral_ulcerations_in_hematopoietic_stem_cell_transplant_recipients_ L2 - https://dx.doi.org/10.1007/s00520-012-1463-2 DB - PRIME DP - Unbound Medicine ER -