Tags

Type your tag names separated by a space and hit enter

Moxibustion inhibits apoptosis and tumor necrosis factor-alpha/tumor necrosis factor receptor 1 in the colonic epithelium of Crohn's disease model rats.
Dig Dis Sci. 2012 Sep; 57(9):2286-95.DD

Abstract

BACKGROUND

Previous studies have shown that moxibustion on Tianshu (ST25) and Qihai (CV6) is effective for treating Crohn's disease. However, the mechanism of moxibustion has not been clearly elucidated.

AIM

The purpose of this study was to investigate the effect of moxibustion on the inhibition of colonic epithelial cell apoptosis and on tumor necrosis factor alpha (TNF-alpha) and tumor necrosis factor receptor TNF receptor-1 (TNFR1) and TNFR2 and to determine the mechanism of its protective effect using Crohn's disease (CD) model rats.

METHODS AND RESULTS

The experimental CD rat models were established by the administration of trinitrobenzene sulfonic acid. In the herbs-partitioned moxibustion (HPM) and mild-warm moxibustion (MWM) groups, moxibustion was administered to Tianshu (ST25) and Qihai (CV6) acupoints once daily for 14 days. In the salicylazosulfapyridine (SASP) group, SASP was administered twice daily for 14 days. A normal control (NC) group and a model control (MC) group were also studied. The levels of TNF-alpha and its mRNA, TNFR1 as well as the rate of colonic epithelial cell apoptosis were significantly decreased in the HPM, MWM and SASP groups compared with the MC group. The HPM and MWM groups had lower mRNA expression and lower protein levels of TNF-alpha compared to the SASP group. The HPM and MWM groups exhibited less apoptosis than the SASP group.

CONCLUSIONS

Moxibustion may inhibit colonic epithelial cell apoptosis by reducing the high expression of TNF-alpha and TNFR1 to protect the defective colonic epithelial barrier in CD model rats.

Authors+Show Affiliations

Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200437, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22531889

Citation

Bao, Chun-Hui, et al. "Moxibustion Inhibits Apoptosis and Tumor Necrosis Factor-alpha/tumor Necrosis Factor Receptor 1 in the Colonic Epithelium of Crohn's Disease Model Rats." Digestive Diseases and Sciences, vol. 57, no. 9, 2012, pp. 2286-95.
Bao CH, Wu LY, Wu HG, et al. Moxibustion inhibits apoptosis and tumor necrosis factor-alpha/tumor necrosis factor receptor 1 in the colonic epithelium of Crohn's disease model rats. Dig Dis Sci. 2012;57(9):2286-95.
Bao, C. H., Wu, L. Y., Wu, H. G., Shi, Y., Liu, H. R., Zhang, R., Yu, L. Q., & Wang, J. H. (2012). Moxibustion inhibits apoptosis and tumor necrosis factor-alpha/tumor necrosis factor receptor 1 in the colonic epithelium of Crohn's disease model rats. Digestive Diseases and Sciences, 57(9), 2286-95. https://doi.org/10.1007/s10620-012-2161-0
Bao CH, et al. Moxibustion Inhibits Apoptosis and Tumor Necrosis Factor-alpha/tumor Necrosis Factor Receptor 1 in the Colonic Epithelium of Crohn's Disease Model Rats. Dig Dis Sci. 2012;57(9):2286-95. PubMed PMID: 22531889.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Moxibustion inhibits apoptosis and tumor necrosis factor-alpha/tumor necrosis factor receptor 1 in the colonic epithelium of Crohn's disease model rats. AU - Bao,Chun-Hui, AU - Wu,Lu-Yi, AU - Wu,Huan-Gan, AU - Shi,Yin, AU - Liu,Hui-Rong, AU - Zhang,Rong, AU - Yu,Li-Qing, AU - Wang,Jin-Hai, Y1 - 2012/04/25/ PY - 2011/04/25/received PY - 2012/03/29/accepted PY - 2012/4/26/entrez PY - 2012/4/26/pubmed PY - 2012/11/7/medline SP - 2286 EP - 95 JF - Digestive diseases and sciences JO - Dig. Dis. Sci. VL - 57 IS - 9 N2 - BACKGROUND: Previous studies have shown that moxibustion on Tianshu (ST25) and Qihai (CV6) is effective for treating Crohn's disease. However, the mechanism of moxibustion has not been clearly elucidated. AIM: The purpose of this study was to investigate the effect of moxibustion on the inhibition of colonic epithelial cell apoptosis and on tumor necrosis factor alpha (TNF-alpha) and tumor necrosis factor receptor TNF receptor-1 (TNFR1) and TNFR2 and to determine the mechanism of its protective effect using Crohn's disease (CD) model rats. METHODS AND RESULTS: The experimental CD rat models were established by the administration of trinitrobenzene sulfonic acid. In the herbs-partitioned moxibustion (HPM) and mild-warm moxibustion (MWM) groups, moxibustion was administered to Tianshu (ST25) and Qihai (CV6) acupoints once daily for 14 days. In the salicylazosulfapyridine (SASP) group, SASP was administered twice daily for 14 days. A normal control (NC) group and a model control (MC) group were also studied. The levels of TNF-alpha and its mRNA, TNFR1 as well as the rate of colonic epithelial cell apoptosis were significantly decreased in the HPM, MWM and SASP groups compared with the MC group. The HPM and MWM groups had lower mRNA expression and lower protein levels of TNF-alpha compared to the SASP group. The HPM and MWM groups exhibited less apoptosis than the SASP group. CONCLUSIONS: Moxibustion may inhibit colonic epithelial cell apoptosis by reducing the high expression of TNF-alpha and TNFR1 to protect the defective colonic epithelial barrier in CD model rats. SN - 1573-2568 UR - https://www.unboundmedicine.com/medline/citation/22531889/Moxibustion_inhibits_apoptosis_and_tumor_necrosis_factor_alpha/tumor_necrosis_factor_receptor_1_in_the_colonic_epithelium_of_Crohn's_disease_model_rats_ L2 - https://doi.org/10.1007/s10620-012-2161-0 DB - PRIME DP - Unbound Medicine ER -