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TRPA1 contributes to the acute inflammatory response and mediates carrageenan-induced paw edema in the mouse.
Sci Rep. 2012; 2:380.SR

Abstract

Transient receptor potential ankyrin 1 (TRPA1) is an ion channel involved in thermosensation and nociception. TRPA1 is activated by exogenous irritants and also by oxidants formed in inflammatory reactions. However, our understanding of its role in inflammation is limited. Here, we tested the hypothesis that TRPA1 is involved in acute inflammatory edema. The TRPA1 agonist allyl isothiocyanate (AITC) induced inflammatory edema when injected intraplantarly to mice, mimicking the classical response to carrageenan. Interestingly, the TRPA1 antagonist HC-030031 and the cyclo-oxygenase (COX) inhibitor ibuprofen inhibited not only AITC but also carrageenan-induced edema. TRPA1-deficient mice displayed attenuated responses to carrageenan and AITC. Furthermore, AITC enhanced COX-2 expression in HEK293 cells transfected with human TRPA1, a response that was reversed by HC-030031. This study demonstrates a hitherto unknown role of TRPA1 in carrageenan-induced inflammatory edema. The results also strongly suggest that TRPA1 contributes, in a COX-dependent manner, to the development of acute inflammation.

Authors+Show Affiliations

The Immunopharmacology Research Group, University of Tampere School of Medicine and Tampere University Hospital, Tampere, Finland.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22532928

Citation

Moilanen, Lauri J., et al. "TRPA1 Contributes to the Acute Inflammatory Response and Mediates Carrageenan-induced Paw Edema in the Mouse." Scientific Reports, vol. 2, 2012, p. 380.
Moilanen LJ, Laavola M, Kukkonen M, et al. TRPA1 contributes to the acute inflammatory response and mediates carrageenan-induced paw edema in the mouse. Sci Rep. 2012;2:380.
Moilanen, L. J., Laavola, M., Kukkonen, M., Korhonen, R., Leppänen, T., Högestätt, E. D., Zygmunt, P. M., Nieminen, R. M., & Moilanen, E. (2012). TRPA1 contributes to the acute inflammatory response and mediates carrageenan-induced paw edema in the mouse. Scientific Reports, 2, 380. https://doi.org/10.1038/srep00380
Moilanen LJ, et al. TRPA1 Contributes to the Acute Inflammatory Response and Mediates Carrageenan-induced Paw Edema in the Mouse. Sci Rep. 2012;2:380. PubMed PMID: 22532928.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - TRPA1 contributes to the acute inflammatory response and mediates carrageenan-induced paw edema in the mouse. AU - Moilanen,Lauri J, AU - Laavola,Mirka, AU - Kukkonen,Meiju, AU - Korhonen,Riku, AU - Leppänen,Tiina, AU - Högestätt,Edward D, AU - Zygmunt,Peter M, AU - Nieminen,Riina M, AU - Moilanen,Eeva, Y1 - 2012/04/24/ PY - 2012/02/20/received PY - 2012/04/05/accepted PY - 2012/4/26/entrez PY - 2012/4/26/pubmed PY - 2012/4/26/medline SP - 380 EP - 380 JF - Scientific reports JO - Sci Rep VL - 2 N2 - Transient receptor potential ankyrin 1 (TRPA1) is an ion channel involved in thermosensation and nociception. TRPA1 is activated by exogenous irritants and also by oxidants formed in inflammatory reactions. However, our understanding of its role in inflammation is limited. Here, we tested the hypothesis that TRPA1 is involved in acute inflammatory edema. The TRPA1 agonist allyl isothiocyanate (AITC) induced inflammatory edema when injected intraplantarly to mice, mimicking the classical response to carrageenan. Interestingly, the TRPA1 antagonist HC-030031 and the cyclo-oxygenase (COX) inhibitor ibuprofen inhibited not only AITC but also carrageenan-induced edema. TRPA1-deficient mice displayed attenuated responses to carrageenan and AITC. Furthermore, AITC enhanced COX-2 expression in HEK293 cells transfected with human TRPA1, a response that was reversed by HC-030031. This study demonstrates a hitherto unknown role of TRPA1 in carrageenan-induced inflammatory edema. The results also strongly suggest that TRPA1 contributes, in a COX-dependent manner, to the development of acute inflammation. SN - 2045-2322 UR - https://www.unboundmedicine.com/medline/citation/22532928/TRPA1_contributes_to_the_acute_inflammatory_response_and_mediates_carrageenan_induced_paw_edema_in_the_mouse_ L2 - https://doi.org/10.1038/srep00380 DB - PRIME DP - Unbound Medicine ER -