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Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay.
J Agric Food Chem 2012; 60(18):4578-85JA

Abstract

The 4-(dimethylamino)cinnamaldehyde (DMAC) assay is currently used to quantify proanthocyanidin (PAC) content in cranberry products. However, this method suffers from issues of accuracy and precision in the analysis and comparison of PAC levels across a broad range of cranberry products. Current use of procyanidin A2 as a standard leads to an underestimation of PACs content in certain cranberry products, especially those containing higher molecular weight PACs. To begin to address the issue of accuracy, a method for the production of a cranberry PAC standard, derived from an extraction of cranberry (c-PAC) press cake, was developed and evaluated. Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were 2.2 times higher than those determined by procyanidin A2. Increased accuracy is critical for estimating PAC content in relationship to research on authenticity, efficacy, and bioactivity, especially in designing clinical trials for determination of putative health benefits.

Authors+Show Affiliations

Department of Food Science, University of Wisconsin-Madison, 1605 Linden Drive, Madison, Wisconsin 53706, United States.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

22533362

Citation

Feliciano, Rodrigo P., et al. "Comparison of Isolated Cranberry (Vaccinium Macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(dimethylamino)cinnamaldehyde Assay." Journal of Agricultural and Food Chemistry, vol. 60, no. 18, 2012, pp. 4578-85.
Feliciano RP, Shea MP, Shanmuganayagam D, et al. Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay. J Agric Food Chem. 2012;60(18):4578-85.
Feliciano, R. P., Shea, M. P., Shanmuganayagam, D., Krueger, C. G., Howell, A. B., & Reed, J. D. (2012). Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay. Journal of Agricultural and Food Chemistry, 60(18), pp. 4578-85. doi:10.1021/jf3007213.
Feliciano RP, et al. Comparison of Isolated Cranberry (Vaccinium Macrocarpon Ait.) Proanthocyanidins to Catechin and Procyanidins A2 and B2 for Use as Standards in the 4-(dimethylamino)cinnamaldehyde Assay. J Agric Food Chem. 2012 May 9;60(18):4578-85. PubMed PMID: 22533362.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Comparison of isolated cranberry (Vaccinium macrocarpon Ait.) proanthocyanidins to catechin and procyanidins A2 and B2 for use as standards in the 4-(dimethylamino)cinnamaldehyde assay. AU - Feliciano,Rodrigo P, AU - Shea,Michael P, AU - Shanmuganayagam,Dhanansayan, AU - Krueger,Christian G, AU - Howell,Amy B, AU - Reed,Jess D, Y1 - 2012/04/30/ PY - 2012/4/27/entrez PY - 2012/4/27/pubmed PY - 2012/9/11/medline SP - 4578 EP - 85 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 60 IS - 18 N2 - The 4-(dimethylamino)cinnamaldehyde (DMAC) assay is currently used to quantify proanthocyanidin (PAC) content in cranberry products. However, this method suffers from issues of accuracy and precision in the analysis and comparison of PAC levels across a broad range of cranberry products. Current use of procyanidin A2 as a standard leads to an underestimation of PACs content in certain cranberry products, especially those containing higher molecular weight PACs. To begin to address the issue of accuracy, a method for the production of a cranberry PAC standard, derived from an extraction of cranberry (c-PAC) press cake, was developed and evaluated. Use of the c-PAC standard to quantify PAC content in cranberry samples resulted in values that were 2.2 times higher than those determined by procyanidin A2. Increased accuracy is critical for estimating PAC content in relationship to research on authenticity, efficacy, and bioactivity, especially in designing clinical trials for determination of putative health benefits. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/22533362/Comparison_of_isolated_cranberry__Vaccinium_macrocarpon_Ait___proanthocyanidins_to_catechin_and_procyanidins_A2_and_B2_for_use_as_standards_in_the_4__dimethylamino_cinnamaldehyde_assay_ L2 - https://dx.doi.org/10.1021/jf3007213 DB - PRIME DP - Unbound Medicine ER -