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Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens.
Lett Appl Microbiol. 2012 Jul; 55(1):62-7.LA

Abstract

AIM

This study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (Ananas comosus), on growth of several agronomically important fungal pathogens.

METHODS AND RESULTS

Purification of bromelain from pineapple stems was carried out by chromatography techniques, and its antimicrobial activity was tested against the fungal pathogens Fusarium verticillioides, Fusarium oxysporum and Fusarium proliferatum by broth microdilution assay. A concentration of 0.3 μmol l(-1) of bromelain was sufficient for 90% growth inhibition of F. verticillioides. The capability of bromelain to inhibit fungal growth is related to its proteolytic activity.

CONCLUSIONS

The study demonstrates that stem bromelain exhibits a potent antifungal activity against phytopathogens and suggests its potential use as an effective agent for crop protection.

SIGNIFICANCE AND IMPACT OF THE STUDY

The results support the use of a natural protease that accumulates at high levels in pineapple stems as alternative to the use of chemical fungicides for crop protection.

Authors+Show Affiliations

Department of Molecular Genetics, Centre for Research in Agricultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Bellaterra, Barcelona, Spain. belen.lopez@cragenomica.esNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22537505

Citation

López-García, B, et al. "Bromelain, a Cysteine Protease From Pineapple (Ananas Comosus) Stem, Is an Inhibitor of Fungal Plant Pathogens." Letters in Applied Microbiology, vol. 55, no. 1, 2012, pp. 62-7.
López-García B, Hernández M, Segundo BS. Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens. Lett Appl Microbiol. 2012;55(1):62-7.
López-García, B., Hernández, M., & Segundo, B. S. (2012). Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens. Letters in Applied Microbiology, 55(1), 62-7. https://doi.org/10.1111/j.1472-765X.2012.03258.x
López-García B, Hernández M, Segundo BS. Bromelain, a Cysteine Protease From Pineapple (Ananas Comosus) Stem, Is an Inhibitor of Fungal Plant Pathogens. Lett Appl Microbiol. 2012;55(1):62-7. PubMed PMID: 22537505.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Bromelain, a cysteine protease from pineapple (Ananas comosus) stem, is an inhibitor of fungal plant pathogens. AU - López-García,B, AU - Hernández,M, AU - Segundo,B S, Y1 - 2012/05/17/ PY - 2012/4/28/entrez PY - 2012/4/28/pubmed PY - 2013/1/12/medline SP - 62 EP - 7 JF - Letters in applied microbiology JO - Lett. Appl. Microbiol. VL - 55 IS - 1 N2 - AIM: This study aimed to evaluate the effect of bromelain, a cysteine protease isolated from pineapple (Ananas comosus), on growth of several agronomically important fungal pathogens. METHODS AND RESULTS: Purification of bromelain from pineapple stems was carried out by chromatography techniques, and its antimicrobial activity was tested against the fungal pathogens Fusarium verticillioides, Fusarium oxysporum and Fusarium proliferatum by broth microdilution assay. A concentration of 0.3 μmol l(-1) of bromelain was sufficient for 90% growth inhibition of F. verticillioides. The capability of bromelain to inhibit fungal growth is related to its proteolytic activity. CONCLUSIONS: The study demonstrates that stem bromelain exhibits a potent antifungal activity against phytopathogens and suggests its potential use as an effective agent for crop protection. SIGNIFICANCE AND IMPACT OF THE STUDY: The results support the use of a natural protease that accumulates at high levels in pineapple stems as alternative to the use of chemical fungicides for crop protection. SN - 1472-765X UR - https://www.unboundmedicine.com/medline/citation/22537505/Bromelain_a_cysteine_protease_from_pineapple__Ananas_comosus__stem_is_an_inhibitor_of_fungal_plant_pathogens_ L2 - https://doi.org/10.1111/j.1472-765X.2012.03258.x DB - PRIME DP - Unbound Medicine ER -