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Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol.
BMC Vet Res. 2012 Apr 26; 8:45.BV

Abstract

BACKGROUND

The pyrethroid class of insecticides, including deltamethrin, is being used as substitutes for organochlorines and organophosphates in pest-control programs because of their low environmental persistence and toxicity. This study was aimed to investigate the impact of commonly used pesticides (deltamethrin) on the blood and tissue oxidative stress level in catfish (Clarias gariepinus); in addition to the protective effect of α-tocopherol on deltamethrin induced oxidative stress. Catfish were divided into three groups, 1st control group include 20 fish divided into two tanks each one contain 10 fish, 2nd deltamethrin group, where Fish exposed to deltamethrin in a concentration (0.75 μg/l) and 3rd Vitamin E group, Fish exposed to deltamethrin and vitamin E at a dose of 12 μg/l for successive 4 days.Serum, liver, kidney and Gills were collected for biochemical assays. Tissue oxidative stress biomarkers malondialdhyde (MDA) and catalase activity in liver, kidney and gills tissues, serum liver enzymes (ALT and AST), serum albumin, total protein, urea and creatinine were analysed.

RESULTS

Our results showed that 48 h. exposure to 0.75 μg/l deltamethrin significantly (p < 0.05) increased lipid peroxidation (MDA) in the liver, kidney and gills while catalase activity was significantly decreased in the same tissues. This accompanied by significant increase in serum ALT, AST activity, urea and creatinine and a marked decrease in serum albumin and total proteins.

CONCLUSIONS

It could be concluded that deltamethrin is highly toxic to catfish even in very low concentration (0.75 μg/l). Moreover the effect of deltamethrin was pronounced in the liver of catfish in comparison with kidneys and gills. Moreover fish antioxidants and oxidative stress could be used as biomarkers for aquatic pollution, thus helping in the diagnosis of pollution. Administration of 12 μg/l α-tocopherol restored the quantified tissue and serum parameters, so supplementation of α-tocopherol consider an effective way to counter the toxicity of deltamethrin in the catfish.

Authors+Show Affiliations

Faculty of Veterinary Medicine, Department of Biochemistry, Beni Suef University, Egypt. kaamin10@yahoo.comNo affiliation info available

Pub Type(s)

Controlled Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22537979

Citation

Amin, Kamal A., and Khalid S. Hashem. "Deltamethrin-induced Oxidative Stress and Biochemical Changes in Tissues and Blood of Catfish (Clarias Gariepinus): Antioxidant Defense and Role of Alpha-tocopherol." BMC Veterinary Research, vol. 8, 2012, p. 45.
Amin KA, Hashem KS. Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. BMC Vet Res. 2012;8:45.
Amin, K. A., & Hashem, K. S. (2012). Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. BMC Veterinary Research, 8, 45. https://doi.org/10.1186/1746-6148-8-45
Amin KA, Hashem KS. Deltamethrin-induced Oxidative Stress and Biochemical Changes in Tissues and Blood of Catfish (Clarias Gariepinus): Antioxidant Defense and Role of Alpha-tocopherol. BMC Vet Res. 2012 Apr 26;8:45. PubMed PMID: 22537979.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Deltamethrin-induced oxidative stress and biochemical changes in tissues and blood of catfish (Clarias gariepinus): antioxidant defense and role of alpha-tocopherol. AU - Amin,Kamal A, AU - Hashem,Khalid S, Y1 - 2012/04/26/ PY - 2011/09/28/received PY - 2012/04/26/accepted PY - 2012/4/28/entrez PY - 2012/4/28/pubmed PY - 2013/3/26/medline SP - 45 EP - 45 JF - BMC veterinary research JO - BMC Vet Res VL - 8 N2 - BACKGROUND: The pyrethroid class of insecticides, including deltamethrin, is being used as substitutes for organochlorines and organophosphates in pest-control programs because of their low environmental persistence and toxicity. This study was aimed to investigate the impact of commonly used pesticides (deltamethrin) on the blood and tissue oxidative stress level in catfish (Clarias gariepinus); in addition to the protective effect of α-tocopherol on deltamethrin induced oxidative stress. Catfish were divided into three groups, 1st control group include 20 fish divided into two tanks each one contain 10 fish, 2nd deltamethrin group, where Fish exposed to deltamethrin in a concentration (0.75 μg/l) and 3rd Vitamin E group, Fish exposed to deltamethrin and vitamin E at a dose of 12 μg/l for successive 4 days.Serum, liver, kidney and Gills were collected for biochemical assays. Tissue oxidative stress biomarkers malondialdhyde (MDA) and catalase activity in liver, kidney and gills tissues, serum liver enzymes (ALT and AST), serum albumin, total protein, urea and creatinine were analysed. RESULTS: Our results showed that 48 h. exposure to 0.75 μg/l deltamethrin significantly (p < 0.05) increased lipid peroxidation (MDA) in the liver, kidney and gills while catalase activity was significantly decreased in the same tissues. This accompanied by significant increase in serum ALT, AST activity, urea and creatinine and a marked decrease in serum albumin and total proteins. CONCLUSIONS: It could be concluded that deltamethrin is highly toxic to catfish even in very low concentration (0.75 μg/l). Moreover the effect of deltamethrin was pronounced in the liver of catfish in comparison with kidneys and gills. Moreover fish antioxidants and oxidative stress could be used as biomarkers for aquatic pollution, thus helping in the diagnosis of pollution. Administration of 12 μg/l α-tocopherol restored the quantified tissue and serum parameters, so supplementation of α-tocopherol consider an effective way to counter the toxicity of deltamethrin in the catfish. SN - 1746-6148 UR - https://www.unboundmedicine.com/medline/citation/22537979/Deltamethrin_induced_oxidative_stress_and_biochemical_changes_in_tissues_and_blood_of_catfish__Clarias_gariepinus_:_antioxidant_defense_and_role_of_alpha_tocopherol_ L2 - https://bmcvetres.biomedcentral.com/articles/10.1186/1746-6148-8-45 DB - PRIME DP - Unbound Medicine ER -