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The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage.
Cells Tissues Organs. 2012; 196(3):241-50.CT

Abstract

OBJECTIVE

To investigate the effects of 3 mycotoxins, deoxynivalenol (DON), nivalenol (NIV) and T-2 toxin, in the presence and absence of selenium (Se) on the metabolism of tissue-engineered cartilage to mimic conditions found in Kashin-Beck disease (KBD) environments.

MATERIALS AND METHODS

Chondrocytes were seeded onto bone matrix gelatin (BMG) to construct engineered cartilage. The 3 toxins were added to the culture media for 3 weeks followed by immunhistochemical analyses of collagens type II and X, aggrecan, matrix metalloproteinases 1 and 3 (MMP-1 and MMP-3), MMP inhibitors 1 and 3 (TIMP-1 and TIMP-3) and α(2) macroglobulin (α2M).

RESULTS

Type II collagen was decreased while type X collagen was increased in response to DON, NIV and T-2 toxin. Aggrecan was reduced by all 3 mycotoxins. Compared with the control, the 3 toxins decreased the expression of α2M, TIMP-1 and TIMP-3, and increased the expression of MMP-1 and MMP-3. Se could partially inhibit the effects of DON, NIV and T-2 toxins.

CONCLUSION

Under the low Se condition, the 3 mycotoxins produced procatabolic changes in cartilage resulting in the loss of aggrecan and type II collagen and promoted a hypertrophic phenotype of chondrocytes characterized by increasing type-X-collagen expression, enhancing the expression of MMPs, while weakening the TIMPs. Se could partially block the effects mentioned above. These results support the hypothesis that the combination of mycotoxin stress and Se deficiency would be the causative factors for KBD.

Authors+Show Affiliations

Institute of Endemic Diseases, Medical School, Xi'an Jiaotong University, Xi'an, PR China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22538829

Citation

Lu, Minling, et al. "The Effects of Mycotoxins and Selenium Deficiency On Tissue-engineered Cartilage." Cells, Tissues, Organs, vol. 196, no. 3, 2012, pp. 241-50.
Lu M, Cao J, Liu F, et al. The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage. Cells Tissues Organs (Print). 2012;196(3):241-50.
Lu, M., Cao, J., Liu, F., Li, S., Chen, J., Fu, Q., Zhang, Z., Liu, J., Luo, M., Wang, J., Li, J., & Caterson, B. (2012). The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage. Cells, Tissues, Organs, 196(3), 241-50. https://doi.org/10.1159/000335046
Lu M, et al. The Effects of Mycotoxins and Selenium Deficiency On Tissue-engineered Cartilage. Cells Tissues Organs (Print). 2012;196(3):241-50. PubMed PMID: 22538829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The effects of mycotoxins and selenium deficiency on tissue-engineered cartilage. AU - Lu,Minling, AU - Cao,Junling, AU - Liu,Fuqiang, AU - Li,Siyuan, AU - Chen,Jinghong, AU - Fu,Qiang, AU - Zhang,Zengtie, AU - Liu,Jiayuan, AU - Luo,Mingxiu, AU - Wang,Jiali, AU - Li,Jin, AU - Caterson,Bruce, Y1 - 2012/04/24/ PY - 2011/11/09/accepted PY - 2012/4/28/entrez PY - 2012/4/28/pubmed PY - 2013/1/18/medline SP - 241 EP - 50 JF - Cells, tissues, organs JO - Cells Tissues Organs (Print) VL - 196 IS - 3 N2 - OBJECTIVE: To investigate the effects of 3 mycotoxins, deoxynivalenol (DON), nivalenol (NIV) and T-2 toxin, in the presence and absence of selenium (Se) on the metabolism of tissue-engineered cartilage to mimic conditions found in Kashin-Beck disease (KBD) environments. MATERIALS AND METHODS: Chondrocytes were seeded onto bone matrix gelatin (BMG) to construct engineered cartilage. The 3 toxins were added to the culture media for 3 weeks followed by immunhistochemical analyses of collagens type II and X, aggrecan, matrix metalloproteinases 1 and 3 (MMP-1 and MMP-3), MMP inhibitors 1 and 3 (TIMP-1 and TIMP-3) and α(2) macroglobulin (α2M). RESULTS: Type II collagen was decreased while type X collagen was increased in response to DON, NIV and T-2 toxin. Aggrecan was reduced by all 3 mycotoxins. Compared with the control, the 3 toxins decreased the expression of α2M, TIMP-1 and TIMP-3, and increased the expression of MMP-1 and MMP-3. Se could partially inhibit the effects of DON, NIV and T-2 toxins. CONCLUSION: Under the low Se condition, the 3 mycotoxins produced procatabolic changes in cartilage resulting in the loss of aggrecan and type II collagen and promoted a hypertrophic phenotype of chondrocytes characterized by increasing type-X-collagen expression, enhancing the expression of MMPs, while weakening the TIMPs. Se could partially block the effects mentioned above. These results support the hypothesis that the combination of mycotoxin stress and Se deficiency would be the causative factors for KBD. SN - 1422-6421 UR - https://www.unboundmedicine.com/medline/citation/22538829/The_effects_of_mycotoxins_and_selenium_deficiency_on_tissue_engineered_cartilage_ L2 - https://www.karger.com?DOI=10.1159/000335046 DB - PRIME DP - Unbound Medicine ER -