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[Analysis of larval excretory-secretory antigen and its immunodiagnosis of Angiostrongyliasis cantonensis infection].
Nan Fang Yi Ke Da Xue Xue Bao. 2012 Apr; 32(4):477-81.NF

Abstract

OBJECTIVE

To analyze the diagnostic value of larval excretory-secretory antigen in Angiostrongylus cantonensis (LESA) infection.

METHODS

A.cantonensis larvae harvested from mice brain were cultured in vitro. The LESA and the adult worm antigens of A.cantonensis (AWA) were collected and analyzed using SDS-PAGE and Western blotting. Two ELISA systems were established using the two antigens (LESA-ELISA and AWA-ELISA) to detect the serum spectra from different sources.

RESULTS

SDS-PAGE and Western blotting displayed fewer protein and antigen bands for LESA than for the adult antigen. Two distinct bands of LESA (with relative molecular masses of 40 000 and 26 000) showed reactivity with the sera from patients with A. cantonensis infection. The serum levels of IgG and IgM antibodies to LESA increased at the beginning of infection in mice, reaching the peak on day 5 after infection and decreased on day 10. Compared with AWA-ELISA, LESA-ELISA showed a lower seropositive ratio in suspected patients with A.cantonensis, with also a lower cross-positive ratio in patients with schistosomiasis and clonorchis sinensis.

CONCLUSION

LESA possesses fewer antigen reaction bands than AWA. Although with a slightly lower positive ratio than AWA, LESA has a higher specificity for detecting serum antibodies in suspected cases of A.cantonensis infection, and therefore shows a potential for the diagnosis of angiostrongyliasis especially in the early stage and in current infection.

Authors+Show Affiliations

Department of Parasitology, School of Public Health and Tropical Medicine, Southern Medical Universtiy, Guangzhou 510515, China. zxcongufo@163.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Comparative Study
English Abstract
Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

22543125

Citation

Zhang, Xin, et al. "[Analysis of Larval Excretory-secretory Antigen and Its Immunodiagnosis of Angiostrongyliasis Cantonensis Infection]." Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, vol. 32, no. 4, 2012, pp. 477-81.
Zhang X, Liu M, Wu Y, et al. [Analysis of larval excretory-secretory antigen and its immunodiagnosis of Angiostrongyliasis cantonensis infection]. Nan Fang Yi Ke Da Xue Xue Bao. 2012;32(4):477-81.
Zhang, X., Liu, M., Wu, Y., Mo, Z., Shen, H., Chen, D., & Li, H. (2012). [Analysis of larval excretory-secretory antigen and its immunodiagnosis of Angiostrongyliasis cantonensis infection]. Nan Fang Yi Ke Da Xue Xue Bao = Journal of Southern Medical University, 32(4), 477-81.
Zhang X, et al. [Analysis of Larval Excretory-secretory Antigen and Its Immunodiagnosis of Angiostrongyliasis Cantonensis Infection]. Nan Fang Yi Ke Da Xue Xue Bao. 2012;32(4):477-81. PubMed PMID: 22543125.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Analysis of larval excretory-secretory antigen and its immunodiagnosis of Angiostrongyliasis cantonensis infection]. AU - Zhang,Xin, AU - Liu,Min, AU - Wu,Yaxin, AU - Mo,Zexun, AU - Shen,Haoxian, AU - Chen,Daixiong, AU - Li,Hua, PY - 2012/5/1/entrez PY - 2012/5/1/pubmed PY - 2013/6/1/medline SP - 477 EP - 81 JF - Nan fang yi ke da xue xue bao = Journal of Southern Medical University JO - Nan Fang Yi Ke Da Xue Xue Bao VL - 32 IS - 4 N2 - OBJECTIVE: To analyze the diagnostic value of larval excretory-secretory antigen in Angiostrongylus cantonensis (LESA) infection. METHODS: A.cantonensis larvae harvested from mice brain were cultured in vitro. The LESA and the adult worm antigens of A.cantonensis (AWA) were collected and analyzed using SDS-PAGE and Western blotting. Two ELISA systems were established using the two antigens (LESA-ELISA and AWA-ELISA) to detect the serum spectra from different sources. RESULTS: SDS-PAGE and Western blotting displayed fewer protein and antigen bands for LESA than for the adult antigen. Two distinct bands of LESA (with relative molecular masses of 40 000 and 26 000) showed reactivity with the sera from patients with A. cantonensis infection. The serum levels of IgG and IgM antibodies to LESA increased at the beginning of infection in mice, reaching the peak on day 5 after infection and decreased on day 10. Compared with AWA-ELISA, LESA-ELISA showed a lower seropositive ratio in suspected patients with A.cantonensis, with also a lower cross-positive ratio in patients with schistosomiasis and clonorchis sinensis. CONCLUSION: LESA possesses fewer antigen reaction bands than AWA. Although with a slightly lower positive ratio than AWA, LESA has a higher specificity for detecting serum antibodies in suspected cases of A.cantonensis infection, and therefore shows a potential for the diagnosis of angiostrongyliasis especially in the early stage and in current infection. SN - 1673-4254 UR - https://www.unboundmedicine.com/medline/citation/22543125/[Analysis_of_larval_excretory_secretory_antigen_and_its_immunodiagnosis_of_Angiostrongyliasis_cantonensis_infection]_ L2 - http://www.diseaseinfosearch.org/result/468 DB - PRIME DP - Unbound Medicine ER -