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Regulation of signaling molecules associated with insulin action, insulin secretion and pancreatic β-cell mass in the hypoglycemic effects of Korean red ginseng in Goto-Kakizaki rats.
J Ethnopharmacol. 2012 Jun 26; 142(1):53-8.JE

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE

Korean red ginseng (KRG) has long history as herbal remedy for antidiabetic effect.

AIM OF THE STUDY

To study molecular mechanisms by which KRG ameliorates diabetes mellitus, we investigated whether the supplementation with the aqueous extract of KRG as a dietary admixture (1%, w/w) regulates the expressions of signaling molecules that are associated with insulin action, insulin secretion and pancreatic β-cell mass in spontaneously diabetic Goto-Kakizaki (GK) rats.

METHODS

An aqueous extract of KRG was supplemented for the estimated dosage to be 0.2 g/kg rat/day beginning at 5 weeks of age for 12 weeks in male GK rats. Plasma glucose levels were measured every 4 weeks. The expressions of signaling molecules that are associated with insulin action, insulin secretion and β-cell mass in tissues were determined by Western blotting.

RESULTS

The 12-week supplementation with KRG significantly (P<0.05) decreased blood glucose compared to control. It up-regulated the expression of glucose transporter (GLUT) 4 in adipose tissue, and down-regulated the expression of protein tyrosine phosphatases (PTP)-1B in adipose tissue and skeletal muscle. It also up-regulated the expression of insulin and down-regulated the expression of uncoupling protein (UCP) 2, Bax and poly (ADP-ribose) polymerase (PARP) in pancreas.

CONCLUSIONS

These results suggest that GLUT4, PTP-1B, insulin, UCP2, Bax and PARP may be the primary targets of KRG that result in increase in insulin action and in insulin secretion, and decrease in β-cell mass, and that cause the normalization in glucose homeostasis.

Authors+Show Affiliations

Division for Metabolism and Functionality Research, Korea Food Research Institute, 516 Baekhyun-dong, Bundang-gu, Songnam-si, Kyonggi-do 463-746, Republic of Korea. khyey@kfri.re.krNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22543177

Citation

Kim, Hye Young, and Kyong Kim. "Regulation of Signaling Molecules Associated With Insulin Action, Insulin Secretion and Pancreatic Β-cell Mass in the Hypoglycemic Effects of Korean Red Ginseng in Goto-Kakizaki Rats." Journal of Ethnopharmacology, vol. 142, no. 1, 2012, pp. 53-8.
Kim HY, Kim K. Regulation of signaling molecules associated with insulin action, insulin secretion and pancreatic β-cell mass in the hypoglycemic effects of Korean red ginseng in Goto-Kakizaki rats. J Ethnopharmacol. 2012;142(1):53-8.
Kim, H. Y., & Kim, K. (2012). Regulation of signaling molecules associated with insulin action, insulin secretion and pancreatic β-cell mass in the hypoglycemic effects of Korean red ginseng in Goto-Kakizaki rats. Journal of Ethnopharmacology, 142(1), 53-8. https://doi.org/10.1016/j.jep.2012.04.012
Kim HY, Kim K. Regulation of Signaling Molecules Associated With Insulin Action, Insulin Secretion and Pancreatic Β-cell Mass in the Hypoglycemic Effects of Korean Red Ginseng in Goto-Kakizaki Rats. J Ethnopharmacol. 2012 Jun 26;142(1):53-8. PubMed PMID: 22543177.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Regulation of signaling molecules associated with insulin action, insulin secretion and pancreatic β-cell mass in the hypoglycemic effects of Korean red ginseng in Goto-Kakizaki rats. AU - Kim,Hye Young, AU - Kim,Kyong, Y1 - 2012/04/18/ PY - 2011/10/11/received PY - 2012/03/23/revised PY - 2012/04/08/accepted PY - 2012/5/1/entrez PY - 2012/5/1/pubmed PY - 2012/10/10/medline SP - 53 EP - 8 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 142 IS - 1 N2 - ETHNOPHARMACOLOGICAL RELEVANCE: Korean red ginseng (KRG) has long history as herbal remedy for antidiabetic effect. AIM OF THE STUDY: To study molecular mechanisms by which KRG ameliorates diabetes mellitus, we investigated whether the supplementation with the aqueous extract of KRG as a dietary admixture (1%, w/w) regulates the expressions of signaling molecules that are associated with insulin action, insulin secretion and pancreatic β-cell mass in spontaneously diabetic Goto-Kakizaki (GK) rats. METHODS: An aqueous extract of KRG was supplemented for the estimated dosage to be 0.2 g/kg rat/day beginning at 5 weeks of age for 12 weeks in male GK rats. Plasma glucose levels were measured every 4 weeks. The expressions of signaling molecules that are associated with insulin action, insulin secretion and β-cell mass in tissues were determined by Western blotting. RESULTS: The 12-week supplementation with KRG significantly (P<0.05) decreased blood glucose compared to control. It up-regulated the expression of glucose transporter (GLUT) 4 in adipose tissue, and down-regulated the expression of protein tyrosine phosphatases (PTP)-1B in adipose tissue and skeletal muscle. It also up-regulated the expression of insulin and down-regulated the expression of uncoupling protein (UCP) 2, Bax and poly (ADP-ribose) polymerase (PARP) in pancreas. CONCLUSIONS: These results suggest that GLUT4, PTP-1B, insulin, UCP2, Bax and PARP may be the primary targets of KRG that result in increase in insulin action and in insulin secretion, and decrease in β-cell mass, and that cause the normalization in glucose homeostasis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/22543177/Regulation_of_signaling_molecules_associated_with_insulin_action_insulin_secretion_and_pancreatic_β_cell_mass_in_the_hypoglycemic_effects_of_Korean_red_ginseng_in_Goto_Kakizaki_rats_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(12)00239-5 DB - PRIME DP - Unbound Medicine ER -