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β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy.
Acta Pharmacol Sin. 2012 Jun; 33(6):737-42.AP

Abstract

AIM

To explore the effects of β-asarone from Acorus Tatarinowii Schott on autophagy in an ischemic stroke model of PC12 cells.

METHODS

The ischemic stroke model of PC12 cells was made by OGD/R (2 h oxygen-glucose deprivation followed by 24 h reperfusion). Drug administration was started 1 h before OGD and last for 3 h. Then the cells were incubated in the drug-free and full culture medium under normoxic conditions for 24 h. After the treatments, Beclin-1, intracellular free calcium concentration ([Ca(2+)](i)) and mitochondrial membrane potential (MMP) were analyzed using flow cytometry. Cell viability was measured using MTT assay. Cell morphology was studied under inverted phase contrast microscope, and autophagosomes were observed under transmission electron microscope.

RESULTS

Pretreatment with β-asarone (20, 30, or 45 μg/mL) or the calcium channel antagonist nimodipine (10 μmol/L) significantly increased the cell viability and MMP, and decreased Beclin-1 expression and [Ca(2+)](i) in OGD/R-treated PC12 cells. Under inverted phase contrast microscope, pretreatment with β-asarone or nimodipine dramatically increase the number of cells and improved the cellular morphology. Autophagosomes were found in OGD/R-treated PC12 cells as well as in drug plus OGD/R-treated PC12 cells.

CONCLUSION

β-Asarone protects PC12 cells against OGD/R-induced injury partly due to attenuating Beclin-1-dependent autophagy caused by decreasing [Ca(2+)](i) and increasing MMP.

Authors+Show Affiliations

The First Affiliated Hospital of Guangzhou University of Chinese Medicine, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22543703

Citation

Mo, Zhen-tao, et al. "Β-Asarone Protects PC12 Cells Against OGD/R-induced Injury Via Attenuating Beclin-1-dependent Autophagy." Acta Pharmacologica Sinica, vol. 33, no. 6, 2012, pp. 737-42.
Mo ZT, Fang YQ, He YP, et al. Β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy. Acta Pharmacol Sin. 2012;33(6):737-42.
Mo, Z. T., Fang, Y. Q., He, Y. P., & Zhang, S. (2012). Β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy. Acta Pharmacologica Sinica, 33(6), 737-42. https://doi.org/10.1038/aps.2012.35
Mo ZT, et al. Β-Asarone Protects PC12 Cells Against OGD/R-induced Injury Via Attenuating Beclin-1-dependent Autophagy. Acta Pharmacol Sin. 2012;33(6):737-42. PubMed PMID: 22543703.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - β-Asarone protects PC12 cells against OGD/R-induced injury via attenuating Beclin-1-dependent autophagy. AU - Mo,Zhen-tao, AU - Fang,Yong-qi, AU - He,Yu-ping, AU - Zhang,Sheng, Y1 - 2012/04/30/ PY - 2012/5/1/entrez PY - 2012/5/1/pubmed PY - 2012/10/16/medline SP - 737 EP - 42 JF - Acta pharmacologica Sinica JO - Acta Pharmacol. Sin. VL - 33 IS - 6 N2 - AIM: To explore the effects of β-asarone from Acorus Tatarinowii Schott on autophagy in an ischemic stroke model of PC12 cells. METHODS: The ischemic stroke model of PC12 cells was made by OGD/R (2 h oxygen-glucose deprivation followed by 24 h reperfusion). Drug administration was started 1 h before OGD and last for 3 h. Then the cells were incubated in the drug-free and full culture medium under normoxic conditions for 24 h. After the treatments, Beclin-1, intracellular free calcium concentration ([Ca(2+)](i)) and mitochondrial membrane potential (MMP) were analyzed using flow cytometry. Cell viability was measured using MTT assay. Cell morphology was studied under inverted phase contrast microscope, and autophagosomes were observed under transmission electron microscope. RESULTS: Pretreatment with β-asarone (20, 30, or 45 μg/mL) or the calcium channel antagonist nimodipine (10 μmol/L) significantly increased the cell viability and MMP, and decreased Beclin-1 expression and [Ca(2+)](i) in OGD/R-treated PC12 cells. Under inverted phase contrast microscope, pretreatment with β-asarone or nimodipine dramatically increase the number of cells and improved the cellular morphology. Autophagosomes were found in OGD/R-treated PC12 cells as well as in drug plus OGD/R-treated PC12 cells. CONCLUSION: β-Asarone protects PC12 cells against OGD/R-induced injury partly due to attenuating Beclin-1-dependent autophagy caused by decreasing [Ca(2+)](i) and increasing MMP. SN - 1745-7254 UR - https://www.unboundmedicine.com/medline/citation/22543703/β_Asarone_protects_PC12_cells_against_OGD/R_induced_injury_via_attenuating_Beclin_1_dependent_autophagy_ L2 - http://dx.doi.org/10.1038/aps.2012.35 DB - PRIME DP - Unbound Medicine ER -