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Development of an enzyme-linked immunosorbent assay for the measurement of antibodies against infectious coryza vaccine.
Avian Dis 2012; 56(1):65-72AD

Abstract

Infectious coryza is an acute respiratory disease caused by infection with Avibacterium (Haemophilus) paragallinarum. It is characterized by nasal discharge and facial swelling and is associated with growth retardation and a reduction in egg production. Hemagglutination inhibition (HI) tests are used to estimate vaccine-induced immunity against infectious coryza in vitro; however, these procedures are complicated and their sensitivity is insufficient. To address these problems, an enzyme-linked immunosorbent assay (ELISA) technique using serovar-specific regions of HMTp210 (210 kDa), an outer-membrane protein of A. paragallinarum, was developed to measure the antibodies against infectious coryza. Chickens with an ELISA titer of 0.3 or more did not exhibit clinical signs of infectious coryza against challenge with A. paragallinarum, although their HI antibody titers were negative. On the other hand, chickens with an ELISA titer below 0.3 exhibited clinical signs of the disease with one exception. Antibody prevalence rates on ELISA were 80% and 60% against infection with serovars A and C, respectively, and ELISA also detected antibodies in chickens infected with A. paragallinarum with a sensitivity higher than that of HI tests. Taken together, the ELISA technique developed in this study is a valuable tool for the measurement of antibodies produced against the infectious coryza vaccine or in response to an infection with A. paragallinarum.

Authors+Show Affiliations

The Chemo-Sero-Therapeutic Research Institute, Kikuchi Research Center, 1314-1 Kawabe Kyokushi, Kikuchi-shi, Kumamoto, 869-1298, Japan. sakamoto-ryu@kaketsuken.or.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Studies
Journal Article

Language

eng

PubMed ID

22545530

Citation

Sakamoto, Ryuichi, et al. "Development of an Enzyme-linked Immunosorbent Assay for the Measurement of Antibodies Against Infectious Coryza Vaccine." Avian Diseases, vol. 56, no. 1, 2012, pp. 65-72.
Sakamoto R, Sakai AT, Ushijima T, et al. Development of an enzyme-linked immunosorbent assay for the measurement of antibodies against infectious coryza vaccine. Avian Dis. 2012;56(1):65-72.
Sakamoto, R., Sakai, A. T., Ushijima, T., Imamura, T., Kino, Y., Honda, T., & Sakaguchi, M. (2012). Development of an enzyme-linked immunosorbent assay for the measurement of antibodies against infectious coryza vaccine. Avian Diseases, 56(1), pp. 65-72.
Sakamoto R, et al. Development of an Enzyme-linked Immunosorbent Assay for the Measurement of Antibodies Against Infectious Coryza Vaccine. Avian Dis. 2012;56(1):65-72. PubMed PMID: 22545530.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Development of an enzyme-linked immunosorbent assay for the measurement of antibodies against infectious coryza vaccine. AU - Sakamoto,Ryuichi, AU - Sakai,A Tomoyo, AU - Ushijima,Toshihiro, AU - Imamura,Takashi, AU - Kino,Yoichiro, AU - Honda,Takashi, AU - Sakaguchi,Masashi, PY - 2012/5/2/entrez PY - 2012/5/2/pubmed PY - 2012/5/23/medline SP - 65 EP - 72 JF - Avian diseases JO - Avian Dis. VL - 56 IS - 1 N2 - Infectious coryza is an acute respiratory disease caused by infection with Avibacterium (Haemophilus) paragallinarum. It is characterized by nasal discharge and facial swelling and is associated with growth retardation and a reduction in egg production. Hemagglutination inhibition (HI) tests are used to estimate vaccine-induced immunity against infectious coryza in vitro; however, these procedures are complicated and their sensitivity is insufficient. To address these problems, an enzyme-linked immunosorbent assay (ELISA) technique using serovar-specific regions of HMTp210 (210 kDa), an outer-membrane protein of A. paragallinarum, was developed to measure the antibodies against infectious coryza. Chickens with an ELISA titer of 0.3 or more did not exhibit clinical signs of infectious coryza against challenge with A. paragallinarum, although their HI antibody titers were negative. On the other hand, chickens with an ELISA titer below 0.3 exhibited clinical signs of the disease with one exception. Antibody prevalence rates on ELISA were 80% and 60% against infection with serovars A and C, respectively, and ELISA also detected antibodies in chickens infected with A. paragallinarum with a sensitivity higher than that of HI tests. Taken together, the ELISA technique developed in this study is a valuable tool for the measurement of antibodies produced against the infectious coryza vaccine or in response to an infection with A. paragallinarum. SN - 0005-2086 UR - https://www.unboundmedicine.com/medline/citation/22545530/Development_of_an_enzyme_linked_immunosorbent_assay_for_the_measurement_of_antibodies_against_infectious_coryza_vaccine_ L2 - http://www.bioone.org/doi/10.1637/9805-052311-Reg.1?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -