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NUR77 inhibits the expression of TIMP2 and increases the migration and invasion of HTR-8/SVneo cells induced by CYR61.
Placenta 2012; 33(7):561-7P

Abstract

OBJECTIVE

To investigate the function and mechanism of CYR61 on the migration and invasion of the trophoblast cell line, HTR-8/SVneo cells.

STUDY DESIGN

The mRNA and protein levels of NUR77 in the placentas of normal and preeclampsia (PE) women were evaluated using real-time PCR and Western blot, respectively. Paraffin-embedded tissues were processed for localization of NUR77 protein in placental villus by immunohistochemistry. HTR-8/SVneo cells were cultured in the presence of CYR61, Ad-NUR77 or a small interfering RNA for NUR77 (Ad-sinur77). The expression of NUR77 in the HTR-8/SVneo cells was detected and the effects of CYR61 on the migration and invasion of HTR-8/SVneo cells were assessed in wound-healing and transwell experiments, respectively. Gelatin zymography was used to measure the MMP2 release in HTR-8/SVneo cells.

RESULTS

NUR77 is significantly decreased in the placenta of women with PE compared with the levels during a normal pregnancy. CYR61 can significantly increase the expression of NUR77 in HTR-8/SVneo cells. CYR61, as well as NUR77, can promote HTR-8/SVneo cells migration and invasion, which can be blocked by Ad-sinur77. Both CYR61 and Ad-nur77 reduced the mRNA expression of TIMP2 in HTR-8/SVneo cells.

CONCLUSIONS

CYR61 may promote HTR-8/SVneo cells migration and invasion through the upregulation of NUR77, leading to the increase of MMP2 release and the downregulation of TIMP2 expression.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Nanjing Drum Tower Hospital, Nanjing University Medical School, Nanjing, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22551568

Citation

Zhang, X, et al. "NUR77 Inhibits the Expression of TIMP2 and Increases the Migration and Invasion of HTR-8/SVneo Cells Induced By CYR61." Placenta, vol. 33, no. 7, 2012, pp. 561-7.
Zhang X, Yan G, Diao Z, et al. NUR77 inhibits the expression of TIMP2 and increases the migration and invasion of HTR-8/SVneo cells induced by CYR61. Placenta. 2012;33(7):561-7.
Zhang, X., Yan, G., Diao, Z., Sun, H., & Hu, Y. (2012). NUR77 inhibits the expression of TIMP2 and increases the migration and invasion of HTR-8/SVneo cells induced by CYR61. Placenta, 33(7), pp. 561-7. doi:10.1016/j.placenta.2012.04.005.
Zhang X, et al. NUR77 Inhibits the Expression of TIMP2 and Increases the Migration and Invasion of HTR-8/SVneo Cells Induced By CYR61. Placenta. 2012;33(7):561-7. PubMed PMID: 22551568.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - NUR77 inhibits the expression of TIMP2 and increases the migration and invasion of HTR-8/SVneo cells induced by CYR61. AU - Zhang,X, AU - Yan,G, AU - Diao,Z, AU - Sun,H, AU - Hu,Y, Y1 - 2012/04/30/ PY - 2011/11/07/received PY - 2012/03/15/revised PY - 2012/04/05/accepted PY - 2012/5/4/entrez PY - 2012/5/4/pubmed PY - 2012/9/25/medline SP - 561 EP - 7 JF - Placenta JO - Placenta VL - 33 IS - 7 N2 - OBJECTIVE: To investigate the function and mechanism of CYR61 on the migration and invasion of the trophoblast cell line, HTR-8/SVneo cells. STUDY DESIGN: The mRNA and protein levels of NUR77 in the placentas of normal and preeclampsia (PE) women were evaluated using real-time PCR and Western blot, respectively. Paraffin-embedded tissues were processed for localization of NUR77 protein in placental villus by immunohistochemistry. HTR-8/SVneo cells were cultured in the presence of CYR61, Ad-NUR77 or a small interfering RNA for NUR77 (Ad-sinur77). The expression of NUR77 in the HTR-8/SVneo cells was detected and the effects of CYR61 on the migration and invasion of HTR-8/SVneo cells were assessed in wound-healing and transwell experiments, respectively. Gelatin zymography was used to measure the MMP2 release in HTR-8/SVneo cells. RESULTS: NUR77 is significantly decreased in the placenta of women with PE compared with the levels during a normal pregnancy. CYR61 can significantly increase the expression of NUR77 in HTR-8/SVneo cells. CYR61, as well as NUR77, can promote HTR-8/SVneo cells migration and invasion, which can be blocked by Ad-sinur77. Both CYR61 and Ad-nur77 reduced the mRNA expression of TIMP2 in HTR-8/SVneo cells. CONCLUSIONS: CYR61 may promote HTR-8/SVneo cells migration and invasion through the upregulation of NUR77, leading to the increase of MMP2 release and the downregulation of TIMP2 expression. SN - 1532-3102 UR - https://www.unboundmedicine.com/medline/citation/22551568/NUR77_inhibits_the_expression_of_TIMP2_and_increases_the_migration_and_invasion_of_HTR_8/SVneo_cells_induced_by_CYR61_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(12)00160-9 DB - PRIME DP - Unbound Medicine ER -