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Simultaneous determination of four plant hormones in bananas by molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography.
Analyst. 2012 Jun 21; 137(12):2884-90.A

Abstract

A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection was developed for the simultaneous isolation and determination of four plant hormones including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) in banana samples. The new molecularly imprinted microspheres (MIMs) prepared by aqueous suspension polymerization using 3-hydroxy-2-naphthoic acid and 1-methylpiperazine as mimic templates performed with high selectivity and affinity for the four plant hormones, and applied as selective sorbents of solid-phase extraction could effectively eliminate the interferences of the banana matrix. Good linearity was obtained in a range of 0.04-4.00 μg g(-1) and the recoveries of the four plant hormones at three spiked levels ranged from 78.5 to 107.7% with the relative standard deviations (RSD) of less than 4.6%. The developed MISPE-HPLC protocol obviously improved the selectivity and eliminated the effect of template leakage on quantitative analysis, and could be applied for the determination of plant hormones in complicated biological samples.

Authors+Show Affiliations

Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmacy, Hebei University, Baoding, 071002, China. yanhy@hbu.edu.cnNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22553770

Citation

Yan, Hongyuan, et al. "Simultaneous Determination of Four Plant Hormones in Bananas By Molecularly Imprinted Solid-phase Extraction Coupled With High Performance Liquid Chromatography." The Analyst, vol. 137, no. 12, 2012, pp. 2884-90.
Yan H, Wang F, Han D, et al. Simultaneous determination of four plant hormones in bananas by molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography. Analyst. 2012;137(12):2884-90.
Yan, H., Wang, F., Han, D., & Yang, G. (2012). Simultaneous determination of four plant hormones in bananas by molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography. The Analyst, 137(12), 2884-90. https://doi.org/10.1039/c2an35362h
Yan H, et al. Simultaneous Determination of Four Plant Hormones in Bananas By Molecularly Imprinted Solid-phase Extraction Coupled With High Performance Liquid Chromatography. Analyst. 2012 Jun 21;137(12):2884-90. PubMed PMID: 22553770.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of four plant hormones in bananas by molecularly imprinted solid-phase extraction coupled with high performance liquid chromatography. AU - Yan,Hongyuan, AU - Wang,Fang, AU - Han,Dandan, AU - Yang,Gengliang, Y1 - 2012/05/03/ PY - 2012/5/4/entrez PY - 2012/5/4/pubmed PY - 2012/10/6/medline SP - 2884 EP - 90 JF - The Analyst JO - Analyst VL - 137 IS - 12 N2 - A highly selective molecularly imprinted solid-phase extraction (MISPE) combined with liquid chromatography-ultraviolet detection was developed for the simultaneous isolation and determination of four plant hormones including indole-3-acetic acid (IAA), indole-3-propionic acid (IPA), indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA) in banana samples. The new molecularly imprinted microspheres (MIMs) prepared by aqueous suspension polymerization using 3-hydroxy-2-naphthoic acid and 1-methylpiperazine as mimic templates performed with high selectivity and affinity for the four plant hormones, and applied as selective sorbents of solid-phase extraction could effectively eliminate the interferences of the banana matrix. Good linearity was obtained in a range of 0.04-4.00 μg g(-1) and the recoveries of the four plant hormones at three spiked levels ranged from 78.5 to 107.7% with the relative standard deviations (RSD) of less than 4.6%. The developed MISPE-HPLC protocol obviously improved the selectivity and eliminated the effect of template leakage on quantitative analysis, and could be applied for the determination of plant hormones in complicated biological samples. SN - 1364-5528 UR - https://www.unboundmedicine.com/medline/citation/22553770/Simultaneous_determination_of_four_plant_hormones_in_bananas_by_molecularly_imprinted_solid_phase_extraction_coupled_with_high_performance_liquid_chromatography_ L2 - https://doi.org/10.1039/c2an35362h DB - PRIME DP - Unbound Medicine ER -