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Her-2/neu and topoisomerase IIα in advanced breast cancer: a comprehensive FISH analysis of 245 cases.
Diagn Mol Pathol. 2012 Jun; 21(2):77-83.DM

Abstract

Her-2/neu gene amplification is an established prognostic factor in breast cancer, and Her-2/neu protein is the target of the therapeutic monoclonal antibody Herceptin. More recently, topoisomerase IIα became a new focus of breast cancer research because of its role as a target for anthracycline therapy. Therefore, we compared Her-2/neu and topoisomerase IIα amplification/deletion in a large series of advanced breast cancer using fluorescence in situ hybridization. Paraffin-embedded archival tissue from 245 patients was retrieved and assessed for Her-2/neu and topoisomerase IIα amplification/deletion by fluorescence in situ hybridization according to standard protocols. Relation to clinical data and survival was sought. A total of 245 cases were analyzed. Amplification for Her-2/neu was seen in 57 cases (23.3%), and for topoisomerase IIα in 12 cases (4.9%). Coamplification was found in 9 samples (3.7%), 3 cases (1.2%) showed amplification of topoisomerase IIα but not of Her-2/neu, and 48 samples (19.8%) displayed amplification for Her-2/neu but not for topoisomerase IIα. Correlation to the histologic type, the stage, or the grade could not be found. Only the amplification of topoisomerase IIα was associated with very poor outcome; survival of cases with amplification of Her-2/neu only was slightly lower than the mean overall survival (27.4 vs. 31.9 mo). Amplification of Her-2/neu and/or topoisomerase IIα is associated with poor overall survival. Amplification of topoisomerase IIα does not seem to be necessarily linked to Her-2/neu-amplification. However, independent determination of these 2 markers seems to be valuable for an individualized therapy of breast cancer patients.

Authors+Show Affiliations

Department of Obstetrics and Gynecology, Campus Innenstadt, Ludwig-Maximilians-University Munich, Munich, Germany. verena.engelstaedter@med.uni-muenchenNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

22555090

Citation

Engelstaedter, Verena, et al. "Her-2/neu and Topoisomerase IIα in Advanced Breast Cancer: a Comprehensive FISH Analysis of 245 Cases." Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, vol. 21, no. 2, 2012, pp. 77-83.
Engelstaedter V, Schiffers J, Kahlert S, et al. Her-2/neu and topoisomerase IIα in advanced breast cancer: a comprehensive FISH analysis of 245 cases. Diagn Mol Pathol. 2012;21(2):77-83.
Engelstaedter, V., Schiffers, J., Kahlert, S., Mainka, P., Engel, J., Kirchner, T., Diebold, J., & Mayr, D. (2012). Her-2/neu and topoisomerase IIα in advanced breast cancer: a comprehensive FISH analysis of 245 cases. Diagnostic Molecular Pathology : the American Journal of Surgical Pathology, Part B, 21(2), 77-83. https://doi.org/10.1097/PDM.0b013e31823b6e43
Engelstaedter V, et al. Her-2/neu and Topoisomerase IIα in Advanced Breast Cancer: a Comprehensive FISH Analysis of 245 Cases. Diagn Mol Pathol. 2012;21(2):77-83. PubMed PMID: 22555090.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Her-2/neu and topoisomerase IIα in advanced breast cancer: a comprehensive FISH analysis of 245 cases. AU - Engelstaedter,Verena, AU - Schiffers,Jennifer, AU - Kahlert,Steffen, AU - Mainka,Patrick, AU - Engel,Jutta, AU - Kirchner,Thomas, AU - Diebold,Joachim, AU - Mayr,Doris, PY - 2012/5/5/entrez PY - 2012/5/5/pubmed PY - 2012/9/6/medline SP - 77 EP - 83 JF - Diagnostic molecular pathology : the American journal of surgical pathology, part B JO - Diagn Mol Pathol VL - 21 IS - 2 N2 - Her-2/neu gene amplification is an established prognostic factor in breast cancer, and Her-2/neu protein is the target of the therapeutic monoclonal antibody Herceptin. More recently, topoisomerase IIα became a new focus of breast cancer research because of its role as a target for anthracycline therapy. Therefore, we compared Her-2/neu and topoisomerase IIα amplification/deletion in a large series of advanced breast cancer using fluorescence in situ hybridization. Paraffin-embedded archival tissue from 245 patients was retrieved and assessed for Her-2/neu and topoisomerase IIα amplification/deletion by fluorescence in situ hybridization according to standard protocols. Relation to clinical data and survival was sought. A total of 245 cases were analyzed. Amplification for Her-2/neu was seen in 57 cases (23.3%), and for topoisomerase IIα in 12 cases (4.9%). Coamplification was found in 9 samples (3.7%), 3 cases (1.2%) showed amplification of topoisomerase IIα but not of Her-2/neu, and 48 samples (19.8%) displayed amplification for Her-2/neu but not for topoisomerase IIα. Correlation to the histologic type, the stage, or the grade could not be found. Only the amplification of topoisomerase IIα was associated with very poor outcome; survival of cases with amplification of Her-2/neu only was slightly lower than the mean overall survival (27.4 vs. 31.9 mo). Amplification of Her-2/neu and/or topoisomerase IIα is associated with poor overall survival. Amplification of topoisomerase IIα does not seem to be necessarily linked to Her-2/neu-amplification. However, independent determination of these 2 markers seems to be valuable for an individualized therapy of breast cancer patients. SN - 1533-4066 UR - https://www.unboundmedicine.com/medline/citation/22555090/Her_2/neu_and_topoisomerase_IIα_in_advanced_breast_cancer:_a_comprehensive_FISH_analysis_of_245_cases_ L2 - https://doi.org/10.1097/PDM.0b013e31823b6e43 DB - PRIME DP - Unbound Medicine ER -