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Counteraction between angiotensin II and angiotensin-(1-7) via activating angiotensin type I and Mas receptor on rat renal mesangial cells.
Regul Pept. 2012 Aug 20; 177(1-3):12-20.RP

Abstract

In the updated concept of renin-angiotensin system (RAS), it contains the angiotensin converting enzyme (ACE)-angiotensin (Ang) II-angtiogensin type 1 receptor (AT1) axis and the angiotensin-converting enzyme-related carboxypeptidase (ACE2)-Ang-(1-7)-Mas axis. The former axis has been well demonstrated performing the vasoconstrictive, proliferative and pro-inflammatory functions by activation of AT1 receptors, while the later new identified axis is considered counterbalancing the effects of the former. The present study is aimed at observing the interaction between Ang-(1-7) and Ang II on cultured rat renal mesangial cells (MCs). RT-PCR, Western blot and immunofluorescent staining and confocal microscopy results showed that both AT1 and Mas receptor were co-distributed in rat renal MCs. Ang-(1-7) showed similar effects on Ang II in cultured MCs that stimulated phosphorylated extracellular signal-regulated kinase (ERK)1/2 phosphorylation and transforms growth factor-β1 synthesis, and cell proliferation and extracellular matrix synthesis. Co-treatment of the cell with Ang-(1-7) and Ang II, Ang-(1-7) counteracted AngII-induced effects in a concentration dependent manner, but failed to alter the changes induced by endothelin-1. The stimulating effect of Ang II was mediated by AT1 receptor while all the effects of Ang-(1-7) were blocked by Mas receptor antagonist A-779, but not by AT1 receptor antagonist losartan or AT2 receptor antagonist PD123319. These results suggest that Ang-(1-7) and Ang II specifically interact with each other on rat renal MCs via activation of their specific receptors, Mas and AT1 receptor respectively.

Authors+Show Affiliations

Department of Physiology and Pathophysiology, Shanghai Medical College, Fudan University, Shanghai 200032, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22561449

Citation

Xue, Hong, et al. "Counteraction Between Angiotensin II and Angiotensin-(1-7) Via Activating Angiotensin Type I and Mas Receptor On Rat Renal Mesangial Cells." Regulatory Peptides, vol. 177, no. 1-3, 2012, pp. 12-20.
Xue H, Zhou L, Yuan P, et al. Counteraction between angiotensin II and angiotensin-(1-7) via activating angiotensin type I and Mas receptor on rat renal mesangial cells. Regul Pept. 2012;177(1-3):12-20.
Xue, H., Zhou, L., Yuan, P., Wang, Z., Ni, J., Yao, T., Wang, J., Huang, Y., Yu, C., & Lu, L. (2012). Counteraction between angiotensin II and angiotensin-(1-7) via activating angiotensin type I and Mas receptor on rat renal mesangial cells. Regulatory Peptides, 177(1-3), 12-20. https://doi.org/10.1016/j.regpep.2012.04.002
Xue H, et al. Counteraction Between Angiotensin II and Angiotensin-(1-7) Via Activating Angiotensin Type I and Mas Receptor On Rat Renal Mesangial Cells. Regul Pept. 2012 Aug 20;177(1-3):12-20. PubMed PMID: 22561449.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Counteraction between angiotensin II and angiotensin-(1-7) via activating angiotensin type I and Mas receptor on rat renal mesangial cells. AU - Xue,Hong, AU - Zhou,Li, AU - Yuan,Ping, AU - Wang,Zhen, AU - Ni,Jun, AU - Yao,Tai, AU - Wang,Jun, AU - Huang,Yu, AU - Yu,Chen, AU - Lu,Limin, Y1 - 2012/05/01/ PY - 2011/08/27/received PY - 2012/03/21/revised PY - 2012/04/23/accepted PY - 2012/5/8/entrez PY - 2012/5/9/pubmed PY - 2012/10/25/medline SP - 12 EP - 20 JF - Regulatory peptides JO - Regul. Pept. VL - 177 IS - 1-3 N2 - In the updated concept of renin-angiotensin system (RAS), it contains the angiotensin converting enzyme (ACE)-angiotensin (Ang) II-angtiogensin type 1 receptor (AT1) axis and the angiotensin-converting enzyme-related carboxypeptidase (ACE2)-Ang-(1-7)-Mas axis. The former axis has been well demonstrated performing the vasoconstrictive, proliferative and pro-inflammatory functions by activation of AT1 receptors, while the later new identified axis is considered counterbalancing the effects of the former. The present study is aimed at observing the interaction between Ang-(1-7) and Ang II on cultured rat renal mesangial cells (MCs). RT-PCR, Western blot and immunofluorescent staining and confocal microscopy results showed that both AT1 and Mas receptor were co-distributed in rat renal MCs. Ang-(1-7) showed similar effects on Ang II in cultured MCs that stimulated phosphorylated extracellular signal-regulated kinase (ERK)1/2 phosphorylation and transforms growth factor-β1 synthesis, and cell proliferation and extracellular matrix synthesis. Co-treatment of the cell with Ang-(1-7) and Ang II, Ang-(1-7) counteracted AngII-induced effects in a concentration dependent manner, but failed to alter the changes induced by endothelin-1. The stimulating effect of Ang II was mediated by AT1 receptor while all the effects of Ang-(1-7) were blocked by Mas receptor antagonist A-779, but not by AT1 receptor antagonist losartan or AT2 receptor antagonist PD123319. These results suggest that Ang-(1-7) and Ang II specifically interact with each other on rat renal MCs via activation of their specific receptors, Mas and AT1 receptor respectively. SN - 1873-1686 UR - https://www.unboundmedicine.com/medline/citation/22561449/Counteraction_between_angiotensin_II_and_angiotensin__1_7__via_activating_angiotensin_type_I_and_Mas_receptor_on_rat_renal_mesangial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0167-0115(12)00059-6 DB - PRIME DP - Unbound Medicine ER -