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Cranberry proanthocyanidins act in synergy with licochalcone A to reduce Porphyromonas gingivalis growth and virulence properties, and to suppress cytokine secretion by macrophages.
J Appl Microbiol 2012; 113(2):438-47JA

Abstract

AIMS

Periodontitis is an inflammatory disease of polymicrobial origin that affects the tooth-supporting tissues. With the spread of antibiotic resistance among pathogenic bacteria, alternative strategies are required to better control infectious diseases such as periodontitis. The aim of our study was to investigate whether two natural compounds, A-type cranberry proanthocyanidins (AC-PACs) and licochalcone A, act in synergy against Porphyromonas gingivalis and the host inflammatory response of a macrophage model.

METHODS AND RESULTS

Using a checkerboard microtitre test, AC-PACs and licochalcone A were found to act in synergy to inhibit P. gingivalis growth and biofilm formation. Fluorescein isothiocyanate-labelled P. gingivalis adhesion to oral epithelial cells was also inhibited by a combination of the two natural compounds in a synergistic manner. Fluorometric assays showed that although AC-PACs and licochalcone A reduced both MMP-9 and P. gingivalis collagenase activities, no synergy was obtained with a combination of the compounds. Lastly, AC-PACs and licochalcone A also acted in synergy to reduce the lipopolysaccharide (LPS)-induced secretion of the pro-inflammatory mediators IL-1β, TNF-α, IL-6 and IL-8 in a macrophage model.

CONCLUSIONS

A-type cranberry proanthocyanidins and licochalcone A, natural compounds from cranberry and licorice, respectively, act in synergy on both P. gingivalis and the host immune response, the two principal etiological factors of periodontitis.

SIGNIFICANCE AND IMPACT OF THE STUDY

The combined use of AC-PACs and licochalcone A may be a potential novel therapeutic strategy for the treatment and prevention of periodontal disease.

Authors+Show Affiliations

Groupe de Recherche en Écologie Buccale, Faculté de Médecine Dentaire, Université Laval, Quebec City, QC, Canada.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

22563853

Citation

Feldman, M, and D Grenier. "Cranberry Proanthocyanidins Act in Synergy With Licochalcone a to Reduce Porphyromonas Gingivalis Growth and Virulence Properties, and to Suppress Cytokine Secretion By Macrophages." Journal of Applied Microbiology, vol. 113, no. 2, 2012, pp. 438-47.
Feldman M, Grenier D. Cranberry proanthocyanidins act in synergy with licochalcone A to reduce Porphyromonas gingivalis growth and virulence properties, and to suppress cytokine secretion by macrophages. J Appl Microbiol. 2012;113(2):438-47.
Feldman, M., & Grenier, D. (2012). Cranberry proanthocyanidins act in synergy with licochalcone A to reduce Porphyromonas gingivalis growth and virulence properties, and to suppress cytokine secretion by macrophages. Journal of Applied Microbiology, 113(2), pp. 438-47. doi:10.1111/j.1365-2672.2012.05329.x.
Feldman M, Grenier D. Cranberry Proanthocyanidins Act in Synergy With Licochalcone a to Reduce Porphyromonas Gingivalis Growth and Virulence Properties, and to Suppress Cytokine Secretion By Macrophages. J Appl Microbiol. 2012;113(2):438-47. PubMed PMID: 22563853.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cranberry proanthocyanidins act in synergy with licochalcone A to reduce Porphyromonas gingivalis growth and virulence properties, and to suppress cytokine secretion by macrophages. AU - Feldman,M, AU - Grenier,D, Y1 - 2012/05/31/ PY - 2012/5/9/entrez PY - 2012/5/9/pubmed PY - 2013/5/23/medline SP - 438 EP - 47 JF - Journal of applied microbiology JO - J. Appl. Microbiol. VL - 113 IS - 2 N2 - AIMS: Periodontitis is an inflammatory disease of polymicrobial origin that affects the tooth-supporting tissues. With the spread of antibiotic resistance among pathogenic bacteria, alternative strategies are required to better control infectious diseases such as periodontitis. The aim of our study was to investigate whether two natural compounds, A-type cranberry proanthocyanidins (AC-PACs) and licochalcone A, act in synergy against Porphyromonas gingivalis and the host inflammatory response of a macrophage model. METHODS AND RESULTS: Using a checkerboard microtitre test, AC-PACs and licochalcone A were found to act in synergy to inhibit P. gingivalis growth and biofilm formation. Fluorescein isothiocyanate-labelled P. gingivalis adhesion to oral epithelial cells was also inhibited by a combination of the two natural compounds in a synergistic manner. Fluorometric assays showed that although AC-PACs and licochalcone A reduced both MMP-9 and P. gingivalis collagenase activities, no synergy was obtained with a combination of the compounds. Lastly, AC-PACs and licochalcone A also acted in synergy to reduce the lipopolysaccharide (LPS)-induced secretion of the pro-inflammatory mediators IL-1β, TNF-α, IL-6 and IL-8 in a macrophage model. CONCLUSIONS: A-type cranberry proanthocyanidins and licochalcone A, natural compounds from cranberry and licorice, respectively, act in synergy on both P. gingivalis and the host immune response, the two principal etiological factors of periodontitis. SIGNIFICANCE AND IMPACT OF THE STUDY: The combined use of AC-PACs and licochalcone A may be a potential novel therapeutic strategy for the treatment and prevention of periodontal disease. SN - 1365-2672 UR - https://www.unboundmedicine.com/medline/citation/22563853/Cranberry_proanthocyanidins_act_in_synergy_with_licochalcone_A_to_reduce_Porphyromonas_gingivalis_growth_and_virulence_properties_and_to_suppress_cytokine_secretion_by_macrophages_ L2 - https://doi.org/10.1111/j.1365-2672.2012.05329.x DB - PRIME DP - Unbound Medicine ER -