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A complementation analysis of mobilization deficient mutants of the plasmid ColE1.
Mol Gen Genet. 1979 May 04; 172(2):211-9.MG

Abstract

Hydroxylamine was used to induce mutants of the ColE1 derived plasmid pML2 that are inefficiently mobilized (Mob-) during conjugation by an Hfr donor. The ability of those mutants to be complemented by deletion mutants and Tn3 insertion mutants of ColE1 was examined. Three complementation groups were identified and localized on the ColE1 genetic map (Mob1, Mob2, and Mob3). One hydroxylamine mutant was not complemented by any mobilization deficient mutant but was complemented by mobilizable ColE1 mutants. Two hydroxylamine mutants were not complemented by any ColE1 derivatives. A mutant that had its relaxation nick site deleted had a markedly reduced mobilizability. The relationship between DNA relaxation nick site deleted had a markedly reduced mobilizability. THe relationship between DNA relaxation, replication and mobilization is considered.

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Publisher Full Text

Authors

Inselburg J
No affiliation info available
Ware P
No affiliation info available

MeSH

ColicinsDNA Topoisomerases, Type IDNA, BacterialEscherichia coliGenetic Complementation TestMutationPhenotypePlasmids

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, P.H.S.

Language

eng

PubMed ID

225640

Citation

Inselburg, J, and P Ware. "A Complementation Analysis of Mobilization Deficient Mutants of the Plasmid ColE1." Molecular & General Genetics : MGG, vol. 172, no. 2, 1979, pp. 211-9.
Inselburg J, Ware P. A complementation analysis of mobilization deficient mutants of the plasmid ColE1. Mol Gen Genet. 1979;172(2):211-9.
Inselburg, J., & Ware, P. (1979). A complementation analysis of mobilization deficient mutants of the plasmid ColE1. Molecular & General Genetics : MGG, 172(2), 211-9.
Inselburg J, Ware P. A Complementation Analysis of Mobilization Deficient Mutants of the Plasmid ColE1. Mol Gen Genet. 1979 May 4;172(2):211-9. PubMed PMID: 225640.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A complementation analysis of mobilization deficient mutants of the plasmid ColE1. AU - Inselburg,J, AU - Ware,P, PY - 1979/5/4/pubmed PY - 1979/5/4/medline PY - 1979/5/4/entrez SP - 211 EP - 9 JF - Molecular & general genetics : MGG JO - Mol Gen Genet VL - 172 IS - 2 N2 - Hydroxylamine was used to induce mutants of the ColE1 derived plasmid pML2 that are inefficiently mobilized (Mob-) during conjugation by an Hfr donor. The ability of those mutants to be complemented by deletion mutants and Tn3 insertion mutants of ColE1 was examined. Three complementation groups were identified and localized on the ColE1 genetic map (Mob1, Mob2, and Mob3). One hydroxylamine mutant was not complemented by any mobilization deficient mutant but was complemented by mobilizable ColE1 mutants. Two hydroxylamine mutants were not complemented by any ColE1 derivatives. A mutant that had its relaxation nick site deleted had a markedly reduced mobilizability. The relationship between DNA relaxation nick site deleted had a markedly reduced mobilizability. THe relationship between DNA relaxation, replication and mobilization is considered. SN - 0026-8925 UR - https://www.unboundmedicine.com/medline/citation/225640/A_complementation_analysis_of_mobilization_deficient_mutants_of_the_plasmid_ColE1_ L2 - https://doi.org/10.1007/BF00268285 DB - PRIME DP - Unbound Medicine ER -